卡维地洛对柯萨奇病毒B3感染小鼠儿茶酚胺及IL-6表达水平的影响

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (12): 1332-1337.

卡维地洛对柯萨奇病毒B3感染小鼠儿茶酚胺及IL-6表达水平的影响

吕干新¹, 张腾², 周娜丹², 陈一和², 革丽莎³, 李岳春²

¹丽水市中心医院心内科,丽水 323000,浙江;
²温州医学院附属第二医院心内科;
³儿科,温州 325027,浙江

收稿日期:2013-03-04 修回日期:2013-11-05 出版日期:2013-12-26 发布日期:2014-01-04
通讯作者: 李岳春,男,硕士,讲师,主要从事病毒性心肌炎的基础与临床研究。Tel: 0577-88002214 E-mail: liyuechun1980@sina.com.cn
作者简介:吕干新,男,本科,副主任医师,主要从事心血管疾病的药物治疗研究。Tel: 13587184118 E-mail: lvganxin@126.com
基金资助:
国家自然科学基金(81200165);浙江省自然科学基金(Y2100551)

Effects of carvedilol on plasma norepinephrine and myocardial IL-6 production in murine model with the coxsackievirus B3-induced viral myocarditis

LV Gan-xin¹, ZHANG Teng², ZHOU Na-dan², CHEN Yi-he², GE Li-Sha³, LI Yue-chun²

¹Department of Cardiology, the Central Hospital of Lishui City, Lishui 323000, Zhejiang, China;
²Department of Cardiology;
³Department of Paediatrics, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325027, Zhejiang, China

Received:2013-03-04 Revised:2013-11-05 Online:2013-12-26 Published:2014-01-04

摘要/Abstract

摘要： 目的: 研究卡维地洛对急性柯萨奇B3(CVB3)病毒性心肌炎小鼠的儿茶酚胺及炎症因子IL-6表达水平的影响。方法: 随机将80只雄性BALB/C小鼠分为3组:正常对照组(n=20),心肌炎组(n=30),卡维地洛组(n=30)。后两组经腹腔接种CVB3诱发急性病毒性心肌炎,感染 24 h 后卡维地洛组每日灌胃给予卡维地洛 10 mg/kg,连续 14 d。于接种第7和14天随机从各组抽取8只小鼠取血后处死并留取心脏等标本。比较干预组与对照组心肌病理改变,采用高效液相色谱-电化学法检测去甲肾上腺素含量,采用RT-PCR法检测心肌IL-6 mRNA的表达,采用酶联免疫吸附实验方法检测心肌IL-6蛋白的表达。结果: 与心肌炎组比较,卡维地洛组心肌病理损伤明显减轻。与正常组比较,病毒性心肌炎小鼠血浆去甲肾上腺素明显升高,卡维地洛干预后小鼠血浆去甲肾上腺素明显下降(P<0.05)。与正常组比较,心肌炎组心肌IL-6表达明显上调(P<0.05)。与心肌炎组比较,卡维地洛组IL-6表达明显下调(P<0.05)。结论: 卡维地洛通过抑制儿茶酚胺对心肌的毒性作用以及下调心肌IL-6表达水平,减轻心肌炎小鼠的心肌损害。

关键词: 卡维地洛, 儿茶酚胺, 白介素-6, 病毒性心肌炎

Abstract: AIM: To examine the effects of carvedilol on plasma norepinephrine and myocardial IL-6 production in murine model with the coxsackievirus B3 (CVB3)-induced viral myocarditis.METHODS: Eighty male BALB/C mice were divided into three groups randomly, including normal group, carvedilol group, myocarditis group. The latter two groups were intraperitoneally inoculated with CVB3. Starting 24 h after infection carvedilol was administered orally in a dose of 10 mg·kg^-1·d^-1 for 14 days continuously. Myocarditis group and normal group were used with same dose PBS. Eight mice who were randomly taken from each groups were killed on day 7 or 14. Myocardial histopathology changes, the heart weight/body weight ratio, the plasma norepinephrine, the mRNA and protein expression of myocardial IL-6 were studied.RESULTS: Carvedilol significantly attenuated myocardial lesions and decreased the heart weight/body weight ratio(P<0.05). The plasma norepinephrine was decreased, the mRNA and protein expression of IL-6 was down regulated remarkably in the carvedilol group compared with the myocarditis group(P<0.05).CONCLUSION: Carvedilol protects against CVB3-induced viral myocarditis in mice. Carvedilol exerts some of its beneficial effects by decreasing the plasma catecholamine levels and down-regulating the expression of IL-6.

Key words: Carvedilol, Norepinephrine, IL-6, Viral myocarditis

中图分类号:

R965.2

吕干新, 张腾, 周娜丹, 陈一和, 革丽莎, 李岳春. 卡维地洛对柯萨奇病毒B3感染小鼠儿茶酚胺及IL-6表达水平的影响[J]. 中国临床药理学与治疗学, 2013, 18(12): 1332-1337.

LV Gan-xin, ZHANG Teng, ZHOU Na-dan, CHEN Yi-he, GE Li-Sha, LI Yue-chun. Effects of carvedilol on plasma norepinephrine and myocardial IL-6 production in murine model with the coxsackievirus B3-induced viral myocarditis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(12): 1332-1337.

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