新型超顺磁性氧化铁在雪旺细胞移植后活体示踪中的应用研究

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (12): 1338-1343.

新型超顺磁性氧化铁在雪旺细胞移植后活体示踪中的应用研究

周江文¹, 谢青松¹, 许信龙¹, 魏晓捷¹, 陈再丰¹, 李立新²

¹慈溪市人民医院神经外科,慈溪 315300,浙江;
²南京医科大学第一附属医院神经外科,南京 210029,江苏

收稿日期:2012-11-19 修回日期:2013-08-09 出版日期:2013-12-26 发布日期:2014-01-04
通讯作者: 谢青松,男,硕士,主治医师,细胞移植修复中枢神经损伤及细胞分子影像。Tel: 0574-63929103 E-mail: qsxie@163.com
作者简介:周江文,男,本科,副主任医师,中枢神经损伤与修复。Tel: 0574-63929103 E-mail: zjw864@hot.mail.com
基金资助:
宁波市自然科学基金资助项目(2010A610070)

Application research of novel Superparamagnetic iron oxide in vivo tracing of transplanted schwann cells

ZHOU Jiang-wen¹, XIE Qing-song¹, XU Xin-long¹, WEI Xiao-jie¹, CHEN Zai-feng¹, LI Li-xin²

¹Department of Neurosurgery, People's Hospital of Cixi City, Cixi 315300, Zhejiang, China;
²Department of Neurosurgery, the First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu, China

Received:2012-11-19 Revised:2013-08-09 Online:2013-12-26 Published:2014-01-04

摘要/Abstract

摘要： 目的: 研究一种新型超顺磁性氧化铁(Superparamagnetic iron oxide,SPIO)在雪旺细胞(SCs)标记及磁共振(MRI)活体示踪中的应用。方法: 以二巯基丁二酸修饰三氧化二铁(Fe₂O₃)配制成一种新型SPIO,用于标记SCs。实验组用SPIO标记SCs,对照组不用SPIO标记,每组各10个样本。对标记后的SCs行普鲁士蓝染色鉴定、MTT测试细胞相对数量。最后将标记后的SCs移植入大鼠脑内,MRI扫描活体示踪。结果: 普鲁士蓝染色发现该SPIO可高效率标记SCs,MTT测试表明实验组与对照组的雪旺细胞相对数量无统计学差异(P＞0.05),MRI检查发现脑内移植的SPIO标记SCs在T₂相上呈明显的低信号。结论: 通过二巯基丁二酸修饰Fe₂O₃获得了新型SPIO,该新型SPIO对细胞增殖及活力无明显影响,不需要转染剂仍可高效率标记SCs,MRI活体显影示踪效果良好。

关键词: 超顺磁性氧化铁, 雪旺细胞, 磁共振成像

Abstract: AIM: To investigate a novel Superparamagnetic iron oxide (SPIO) used for labeling Schwann cells (SCs) and tracing in vivo by magnetic resonance.METHODS: The novel SPIO was prepared with Fe₂O₃ coated by a thin layer of dimercaptosuccinic acid, and then it was used for labeling SCs. The SCs of trial group were labeled by SPIO. The SCs of control group weren't labeled by SPIO. Every group had ten samples. The labeled SCs were identified by Prussian blue stain and counted by MTT. Lastly, the labeled SCs was transplanted into rat brain and scanned by MRI.RESULTS: Prussian blue stain indicated that SCs could be high efficiently labeled by the novel SPIO. MTT detection suggested the difference of viable cells relative number was not significant between trial group and control group (P＞0.05). Hypo-intense changes of MRI T₂ examination could be observed in the transplanted area of rat brainlabeled by the new SPIO after transplantation in rats brain.CONCLUSION: The novel Superparamagnetic iron oxide had no significant effect on the proliferation and viability of SCs. SCs still could be labelled with the novel SPIO directly and high efficiently without the use of poly-L-lysine or other additional transfecting agents. The transplanted cells could be well imaged and traced in vivo.

Key words: Superparamagnetic iron oxide, Schwann cells, Magnetic resonance imaging

中图分类号:

R965.2

周江文, 谢青松, 许信龙, 魏晓捷, 陈再丰, 李立新. 新型超顺磁性氧化铁在雪旺细胞移植后活体示踪中的应用研究[J]. 中国临床药理学与治疗学, 2013, 18(12): 1338-1343.

ZHOU Jiang-wen, XIE Qing-song, XU Xin-long, WEI Xiao-jie, CHEN Zai-feng, LI Li-xin. Application research of novel Superparamagnetic iron oxide in vivo tracing of transplanted schwann cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(12): 1338-1343.

参考文献

[1] Bunge MB, Wood PM. Realizing the maximum potential of Schwann cells to promote recovery from spinal cord injury[J]. Handb Clin Neurol, 2012, 10(9): 523-540.
[2] Mantovani C, Terenghi G, Shawcross SG. Isolation of adult stem cells and their differentiation to schwann cells[J]. Methods Mol Biol, 2012, 9(16): 47-57.
[3] Zhang S, Chen XJ, Gu CR, et al. The effect of iron oxide magnetic nanoparticles on smooth muscle cells[J]. Nanoscale Res Lett, 2009, 4(10): 70-77.
[4] Qin JB, Li KA, Li XX, et al. Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury [J]. Int J Nanomedicine, 2012, 7(10): 5191-5203.
[5] 谢青松, 许信龙, 魏晓捷, 等. 新型超顺磁性氧化铁在干细胞活体示踪中的应用研究[J]. 中国临床药理学与治疗学, 2010, 15(3): 272-276.
[6] Al-Deen FN, Selomulya C, Williams T. On designing stable magnetic vectors as carriers for malaria DNA vaccine[J]. Colloids Surf B Biointerfaces, 2012, 10(2): 492-503.
[7] Petri-Fink A, Rothen-Rutishauser B. Nanoparticles and cells: an interdisciplinary approach[J]. Chimia, 2012, 66(3): 104-109.
[8] Wang C, Ravi S, Martinez GV, et al. Dual-purpose magnetic micelles for MRI and gene delivery[J]. J Control Release, 2012, 163(1): 82-92.
[9] Arbab AS, Liu W, Frank JA. Cellular magnetic resonance imaging: current status and future prospects[J]. Expert Rev Med Devices, 2006, 3(4): 427-439.
[10] Wang YX, Hussain SM, Krestin GP. Superparamagnetic iron oxide contrast agents: physicochemical characteristics and applications in MR imaging[J]. Eur Radiol, 2001, 11(11): 2319-2331.
[11] Ramaswamy S, Greco JB, Uluer MC, et al. Magnetic resonance imaging of chondrocytes labeled with superparamagnetic iron oxide nanoparticles in tissue-engineered cartilage[J]. Tissue Eng Part A, 2009, 15(12): 3899-3910.