羧胺三唑对角叉菜胶诱导急性炎症的抗炎作用

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (6): 601-605.

• 基础研究 • 下一篇

羧胺三唑对角叉菜胶诱导急性炎症的抗炎作用

朱蕾, 李娟, 武丹威, 于晓丽, 郭磊, 叶菜英, 张德昌

中国医学科学院,基础医学研究所&北京协和医学院基础学院药理系,北京 100005

收稿日期:2013-02-11 修回日期:2013-05-15 发布日期:2013-06-19
通讯作者: 叶菜英,女,教授,博士生导师,研究方向:抗炎免疫药理学、肿瘤药理学。Tel: 010-65597159 E-mail: caiyingye@126.com;张德昌,男,教授,博士生导师,研究方向:抗炎免疫药理学、肿瘤药理学。Tel: 010-69156401 E-mail: zhangdechang45@vip.sina.com
作者简介:朱蕾,女,博士,助理研究员,研究方向:抗炎免疫药理学。Tel: 010-69156402 E-mail: leizhu2004@126.com
基金资助:
国家自然科学基金(81102454,30873075);“重大新药创制”科技重大专项(2009ZX09102-049);中国医学科学院院所长基金(2010PY01,2010PY08)

Anti-inflammatory effects of carboxyamidotriazole on carrageenan-induced acute inflammation

ZHU Lei, LI Juan, WU Dan-wei, YU Xiao-li, GUO Lei, YE Cai-ying, ZHANG De-chang

Department of Pharmacology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College, Beijing 100005, China

Received:2013-02-11 Revised:2013-05-15 Published:2013-06-19

摘要/Abstract

摘要： 目的: 探讨羧胺三唑(CAI)对角叉菜胶诱导的大鼠足爪急性炎症的预防作用及部分机制。方法: 采用角叉菜胶诱导大鼠足爪急性炎症模型,测量足爪肿胀度以及足爪组织中髓过氧化物酶(MPO)、NO、前列腺素2(PGE₂)、TNF-α、IL-1β、中性粒细胞趋化因子-1(CNCI-1)和诱导一氧化氮合酶(iNOS)。结果: CAI能明显减轻大鼠足爪炎性肿胀,显著降低炎症足爪中MPO、NO、PGE₂、TNF-α、IL-1β和CNCI-1含量及iNOS蛋白表达。结论: CAI可能通过减少炎症介质和中性粒细胞浸润来发挥对角叉菜胶诱导急性炎症的抗炎作用。

关键词: 羧胺三唑, 急性炎症, 角叉菜胶, 髓过氧化物酶, 炎症介质

Abstract: AIM: To investigate the preventive effects of carboxyamidotriazole (CAI) in rat model of carrageenan-induced paw acute inflammation and its mechanisms.METHODS: The paw acute inflammation in rats was induced by carrageenan. The paw swelling was measured, and myeloperoxidase (MPO), NO, PGE₂, TNF-α, IL-1β, cytokine-induced neutrophil chemoattractant-1 (CNCI-1) and iNOS in the paw tissues were determined.RESULTS: CAI attenuated the carrageenan-induced paw swelling. CAI decreased the contents of MPO, NO, PGE₂, TNF-α, IL-1β and CNCI-1 in the inflamed paw tissues. Also, the protein expression of iNOS was reduced by CAI.CONCLUSION: CAI has anti-inflammatory effects against carrageenan-induced acute inflammation by decreasing the inflammatory mediators and the neutrophils infiltration.

Key words: Carboxyamidotriazole, Acute inflammation, Carrageenan, Myeloperoxidase, Inflammatory mediators

中图分类号:

R965.2

朱蕾, 李娟, 武丹威, 于晓丽, 郭磊, 叶菜英, 张德昌. 羧胺三唑对角叉菜胶诱导急性炎症的抗炎作用[J]. 中国临床药理学与治疗学, 2013, 18(6): 601-605.

ZHU Lei, LI Juan, WU Dan-wei, YU Xiao-li, GUO Lei, YE Cai-ying, ZHANG De-chang. Anti-inflammatory effects of carboxyamidotriazole on carrageenan-induced acute inflammation[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(6): 601-605.

参考文献

[1] Moody TW, Chiles J, Moody E, et al. CAI inhibits the growth of small cell lung cancer cells[J]. Lung Cancer, 2003, 39(3): 279-288.
[2] Guo L, Ye CY, Chen WY, et al. Anti-inflammatory and analgesic potency of carboxyamidotriazole, a tumoristatic agent[J]. J Pharmacol Exp Ther, 2008, 325(1): 10-16.
[3] Guo L, Ye C, Hao X, et al. Carboxyamidotriazole ameliorates experimental colitis by inhibition of cytokine production, nuclear factor-κB activation, and colonic fibrosis[J]. J Pharmacol Exp Ther, 2012, 342(2): 356-365.
[4] Salvemini D, Wang ZQ, Wyatt PS, et al. Nitric oxide: a key mediator in the early and late phase of carrageenan-induced rat paw inflammation[J]. Br J Pharmacol, 1996, 118(4): 829-838.
[5] Sakaguchi Y, Shirahase H, Kunishiro K, et al. Synergistic effect of nitric oxide synthase and cyclooxygenase inhibitors on carrageenan-induced paw edema in rats[J]. Arzneimittelforschung, 2006, 56(10): 695-699.
[6] 郑茹,郝晓健,郭磊,等. 羧胺三唑抑制大鼠腹腔巨噬细胞iNOS表达和NF-κB活化[J]. 基础医学与临床, 2010, 30(5): 466-470.
[7] Higgins AJ,Lees P.The acute inflammatory process,arachidonic acid metabolism and the mode of action of anti-inflammatory drugs[J]. Equine Vet J, 1984, 16(3): 163-175.
[8] 蒋柳明,金建国,祝卿,等. 异丙酚对油酸型急性肺损伤兔TNF-α、IL-1β的影响[J]. 中国临床药理学与治疗学, 2011, 16(9): 1002-1005.
[9] 陈杰,李瑞明,许静,等. 蟛蜞菊内酯对脂多糖诱导RAW264.7细胞环氧化酶2、NO及TNF-α的影响[J]. 中国临床药理学与治疗学, 2012, 17(2): 171-174.
[10]Koto H, Salmon M, Haddad el-B, et al. Role of cytokine-induced neutrophil chemoattractant (CNCI) in ozone-induced airway inflammation and hyperresponsiveness[J]. Am J Respir Crit Care Med, 1997, 156(1): 234-239.

羧胺三唑对角叉菜胶诱导急性炎症的抗炎作用

Anti-inflammatory effects of carboxyamidotriazole on carrageenan-induced acute inflammation

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