鬼针草总黄酮对急性炎症的保护作用及可能机制研究

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (6): 614-620.

鬼针草总黄酮对急性炎症的保护作用及可能机制研究

林梅英, 陈飞虎, 葛金芳, 唐杰, 倪文琳

安徽医科大学药学院,合肥 230032,安徽

收稿日期:2012-11-19 修回日期:2013-05-12 发布日期:2013-06-19
通讯作者: 陈飞虎,男,博士,教授,博士生导师,研究方向:临床药理学,抗炎免疫药理学。Tel: 0551-5161115 E-mail: cfhchina@sohu.com
作者简介:林梅英,女,硕士,研究方向:分子药理学。Tel: 13865960890 E-mail: linmeiyingfujian@163.com
基金资助:
国家科技重大专项重大新药创制项目(2009ZX09102-386)

Protective effects of total flavonoids of Bidens bipinnata L on acute inflammation and possible mechanism

LIN Mei-ying, CHEN Fei-hu, GE Jin-fang, TANG Jie, NI Wen-lin

School of Pharmacy, Anhui Medical University, Hefei 230032, Anhui, China

Received:2012-11-19 Revised:2013-05-12 Published:2013-06-19

摘要/Abstract

摘要： 目的: 研究鬼针草总黄酮(TFB)对急性炎症的保护作用与可能机制。方法: 二甲苯诱导急性小鼠耳肿胀,检测耳肿胀度和血清TNF-α、IL-1、IL-6、IL-8含量;氟氏完全佐剂诱导佐剂性关节炎(AA)大鼠原发性炎症,检测足肿胀度和血清TNF-α、IL-1、IL-2、IL-6、IL-8含量,HE染色观察炎症关节病理学变化,免疫组织化学法检测关节软骨组织ASIC1a蛋白。结果: 小鼠急性耳肿胀和AA大鼠原发性炎症中,模型组动物血清TNF-α、IL-1、IL-6和IL-8含量均明显升高,TFB(100、200 mg/kg) 灌胃给药能降低小鼠耳肿胀度和血清TNF-α、IL-1、IL-6、IL-8含量,TFB(67、133 mg/kg)可以升高AA大鼠血清IL-2的含量。TFB灌胃给药可以使AA大鼠关节炎性细胞减少,改善病理变化。在AA大鼠原发性炎症中模型组大鼠关节软骨组织ASIC1a表达明显升高,TFB(67、133 mg/kg)组ASIC1a的表达明显降低。相关性分析结果显示,AA大鼠原发性炎症中ASIC1a与血清TNF-α、IL-1、IL-8含量呈正相关。结论: TFB对急性炎症具有保护作用,其抗炎机制可能与调节炎症介质释放有关。

关键词: 鬼针草总黄酮, 急性炎症, 酸敏感离子通道(ASICs)

Abstract: AIM: To investigate the protective effects of total flavones of Bidens bipinnata L (TFB) on acute inflammation and its possible mechanism.METHODS: Mice ear edema model was induced by dimethylbenzene, and ear edema degree was measured 30 min later. Adjuvant arthritis (AA) rats model was established through intradermal injected with freund's complete adjuvant, and paw swelling degree was observed. Serum TNF-α, IL-1, IL-6, IL-8, and IL-2 contents were detected through enzyme linked immunosorbent assay (ELISA) in both models. Histopathological changes of knee joint were measured through hematoxylin-eosin staining, and the protein expression of ASIC1a in cartilage tissue was tested through immunohistochemistry.RESULTS: Compared with that of the model group, the ear edema degree and serum TNF-α, IL-1, IL-6, and IL-8 contents in the TFB (100, 200 mg/kg) groups were decreased. Compared with that of the AA model rats, the paw swelling degree and the protein expression of ASIC1a in cartilage tissue were lower in TFB (67, 133 mg/kg) groups, while serum IL-2 contents were elevated. The histopathological changes in the knee joint of AA rats including the number of inflammatory cells could be ameliorated by TFB(33, 67, 133 mg/kg). Results of Person correlation test demonstrated that ASIC1a protein was positively correlated with serum TNF-α, IL-1 and IL-8 concentrations.CONCLUSION: TFB has protective effects on acute inflammation, the mechanism might be associated with inhibitting the release of inflammatory mediators.

Key words: Total flavones of Bidens bipinnata L, Acute inflammation, ASICs

中图分类号:

R965.2

林梅英, 陈飞虎, 葛金芳, 唐杰, 倪文琳. 鬼针草总黄酮对急性炎症的保护作用及可能机制研究[J]. 中国临床药理学与治疗学, 2013, 18(6): 614-620.

LIN Mei-ying, CHEN Fei-hu, GE Jin-fang, TANG Jie, NI Wen-lin. Protective effects of total flavonoids of Bidens bipinnata L on acute inflammation and possible mechanism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(6): 614-620.

参考文献

[1] 胡伟, 陈飞虎, 吴繁荣, 等. 鬼针草药材的生药学鉴定[J]. 时珍国医国药, 2009, 20(2): 329-330.
[2] 王琪, 张燕娜, 陈飞虎. 鬼针草总黄酮的化学成分及药理作用研究进展[J]. 安徽医药, 2009, 13(9): 1011-1013.
[3] Yuan LP, Chen FH, Zhong MM, et al. Protective effects of total flavonoids of Bidens bipinnata L. against carbon tetrachloride-induced liver fibrosis in rats [J]. J Pharm Pharmacol, 2008, 60(10): 1393-1402.
[4] Zhong MM, Chen FH, Yuan LP, et al. Protective effect of total flavonoids from Bidens bipinnata L. against carbon tetrachloride-induced liver injury in mice[J]. J Pharm Pharmacol,2007, 59(7): 1017-1025.
[5] Deval E, Gasull X, Noёl J, et al. Acid-sensing ion channels (ASICs): pharmacology and implication in pain[J]. Pharmacol Ther, 2010, 128(3): 549-558.
[6] Shimada S, Ueda T, Ishida Y, et al. Acid-sensing ion channels in taste buds[J]. Arch Histol Cytol, 2006, 69(4): 227-231.
[7] Kuduk SD, Chang RK, Wai JM, et al. Amidine derived inhibitors of acid-sensing ion channel-3 (ASIC3)[J]. Bioorg Med Chem Lett, 2009, 19(15): 4059-4063.
[8] 陈飞虎, 袁凤来, 李霞, 等. 大鼠关节软骨酸敏感离子通道的表达[J]. 安徽医科大学学报, 2007, 42(5): 513-516.
[9] 陈飞虎, 袁凤来, 李霞, 等. 重组人内抑素对佐剂性关节炎大鼠关节软骨酸敏感离子通道的影响及其保护作用[J]. 中国药理学通报, 2008, 24(7): 893-897.
[10]Ekambaram S, Peruma SS, Subramanian V. Evaluation of antiarthritic activity of Strychnos potatorum Linn seeds in Freund's adjuvant induced arthritic rat model[J]. BMC Complement Altern Med, 2010, 10: 56.
[11]Yang T, Wang Z, Wu F, et al. A variant of TNFR2-Fc fusion protein exhibits improved efficacy in treating experimental rheumatoid arthritis A variant of TNFR2-Fc fusion protein exhibits improved efficacy in treating experimental rheumatoid arthritis [J]. PLoS Comput Biol, 2010, 6(2): e1000669.
[12]Brandão FM, Coelho P, Pinto P, et al. Tocilizumab-a new step in rheumatoid arthritis treatment [J]. Acta Reumatol Port, 2010, 35(3): 302-312.
[13]万启南, 曹东, 叶勇, 等. 清热解毒扶正颗粒抗炎、退热及对C-反应蛋白影响的实验研究[J]. 云南中医中药杂志, 2011, 32(3): 47-49.
[14]朱京虎, 洪孝民, 杨海人, 等. 关节康口服液对大鼠佐剂性关节炎的治疗作用及胃黏膜保护作用研究[J]. 药物研究, 2007, 16(22): 26-27.
[15]Bitencourt MA, Dantas GR, Lira DP, et al. Aqueous and methanolic extracts of Caulerpa mexicana suppress cell migration and ear edema induced by inflammatory agents[J]. Mar Drugs, 2011, 9(8): 1332-1345.
[16]张月琴. 雷公藤多苷抗炎作用的研究[J]. 医药导报, 2012, 31(3): 295-297.
[17]何颖, 杨桂林, 胡祥宇, 等. 青果总黄酮的抗炎作用研究[J]. 安徽农业科学, 2012, 4(5): 2632-2635.
[18]Franks AL, Slansky JE. Multiple associations between a broad spectrum of autoimmune diseases, chronic inflammatory diseases and cancer[J]. Anticancer Res, 2012, 32(4): 1119-1136.
[19]汤迎爽, 张红宾, 康阿龙. 鬼针草的化学成分与药理作用研究进展[J]. 中医药导报, 2010, 11(16): 123-125.
[20]蒋海强, 王建平, 范辉. 鬼针草黄酮抗氧化研究[J]. 中药园地, 2010, 3(29): 196-197.
[21]葛金芳, 李俊, 姚宏伟, 等. 枇杷叶三萜酸的抗炎作用[J]. 安徽医科大学学报, 2007, 42(2): 174-178,214.
[22]Hu XJ, Jin HZ, Xu WZ, et al. Anti-inflammatory and analgesic activities of Edgeworthia chrysantha and its effective chemical constituents[J]. Biol Pharm Bull, 2008, 31(9): 1761-1765.
[23]Okoli CO, Akah PA, Onuoha NJ, et al. Acanthus montanus: an experimental evaluation of the antimicrobial, anti-inflammatory and immunological properties of a traditional remedy for furuncles[J]. BMC Complement Altern Med, 2008, 8: 27.