TKTL1在星形胶质细胞瘤中的表达及其意义

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (6): 638-642.

TKTL1在星形胶质细胞瘤中的表达及其意义

李曙¹, 洪云², 张根葆¹, 杨士勇³, 周岩方⁴, 陶帮宝⁵

¹皖南医学院病理生理学教研室,
²弋矶山医院超声医学科,
³神经外科,芜湖 241002,安徽,
⁴温州医学院附属黄岩医院神经外科,台州318020,浙江,
⁵上海交通大学医学院附属新华医院神经外科,上海 200092

收稿日期:2013-04-03 修回日期:2013-06-03 发布日期:2013-06-19
通讯作者: 周岩方,男,副主任医师, 研究方向:肿瘤生物学特性。E-mail: 1304194299@qq.com
作者简介:李曙,男,博士在读,讲师,研究方向:肿瘤生物学特性。Tel: 0553-3932435 E-mail: yxx2003@126.com
基金资助:
安徽省高等学校省级自然科学研究项目(KJ2013Z339);安徽省高等学校省级教学研究项目(2012jyxm327);安徽省教育厅自然基金重点项目 (KJ2011266)

Expression and significance of TKTL1 in glioma

LI Shu¹, HONG Yun², ZHANG Gen-bao¹, YANG Shi-yong³, ZHOU Yan-fang⁴, TAO Bang-bao⁵

¹Department of Pathophysiology,
²Department of Ultrasonography,
³Department of Neurosurgery,Yijishan Hospital,Wannan Medical College,Wuhu 241002,Anhui,China,
⁴Department of Neurosurgery,Huangyan Hospital Affiliated to Wenzhou Medical College,Taizhou 318020,Zhejaing,China,
⁵Department of Neurosurgery,Xinhua Hospital,Shanghai Jiaotong University,Shanghai 200092,China

Received:2013-04-03 Revised:2013-06-03 Published:2013-06-19

摘要/Abstract

摘要： 目的: 探讨转酮醇酶样基因1(Transketolase-like gene1, TKTL1)在胶质瘤中的表达及意义。方法: 收集54例手术切除的胶质瘤标本,6例正常脑组织,通过Realtime-PCR、Western blot及免疫组化方法,从mRNA和蛋白水平检测TKTL1在54例胶质瘤、6例正常垂体组织的表达。结果: Realtime-PCR、Western blot及免疫组化发现TKTL1在胶质瘤组织其表达显著高于正常脑组织(P<0.01),在高级别胶质瘤中和低级别胶质瘤中的蛋白及mRNA表达量两者之间差异具有统计学意义 (P<0.01)。结论: TKTL1的表达与肿瘤恶性程度密切相关,表明TKTL1的表达与肿瘤的发生、发展密切相关,提示TKTL1有可能是预测胶质瘤预后的重要指标。

关键词: TKTL1, 胶质瘤, 预后

Abstract: AIM: To investigate the expression and significance of TKTL1 in glioma.METHODS: Fifty-four gliomas were collected immediately after surgery together with six normal brain tissues. The expression of TKTL1 in fifty-four gliomas and six normal pituitary tissues in terms of the mRNA and protein levels were detected by Realtime-PCR, Western blot and Immunohistochemistry analysis.RESULTS: The expression of TKTL1 in all glioma tissues was higher than that in normal brian tissues by wester blot and Realtime-PCR analysis (P<0.01), the expression amounts of protein and mRNA in high-grade gliomas and low-grade gliomas respectively were significantly different (P<0.01).CONCLUSION: The results reveal that the expression of TKTL1 is closely correlated with malignant degree of glioma, which indicates the expression of TKTL1 is intimately correlated with occurrence and development of glioma. Detecting TKTL1 may be a vital index to predict prognosis of glioma.

Key words: TKTL1, Glioma, Prognosis

中图分类号:

R965.2

李曙, 洪云, 张根葆, 杨士勇, 周岩方, 陶帮宝. TKTL1在星形胶质细胞瘤中的表达及其意义[J]. 中国临床药理学与治疗学, 2013, 18(6): 638-642.

LI Shu, HONG Yun, ZHANG Gen-bao, YANG Shi-yong, ZHOU Yan-fang, TAO Bang-bao. Expression and significance of TKTL1 in glioma[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(6): 638-642.

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