关键词: 前列腺素E2, 环氧化酶, 心肌细胞, 细胞肥大

Abstract: AIM: To explore the effects of PGE2 on cardiomyocyte through experiments on the cultured H9c2 cardiac cells, assess the dose-effect relationship to provide the basic theory for looking for potential targets of inhibiting myocardial hypertrophy.METHODS: H9c2 cardiac cells were randomly divided into four groups: control group (group C), 0.1 μmol/L PGE2 group (group D1), 1 μmol/L PGE2 group (group D2) and 10 μmol/L PGE2 group (group D3). Forty-eight hours later after PGE2 was fed, the morphology of H9c2 cardiac cells were recorded by using a fluorescence microscope. The occurrence of myocardial hypertrophy was observed in terms of cell diameter and total protein of the cells. And the gene expression levels of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) mRNA in cells, which were measured by RT-PCR, helped to judge whether there was an occurrence of pathological myocardial hypertrophy.RESULTS: Compared with group C, the cell shape changed and the volume increased, the cell diameter and the total protein were increased in group D1, group D2 and group D3 (P<0.05). There were also significant differences between different doses of PGE2 (P<0.05); the expression levels of ANP and BNP mRNA were upregulated in group D2 and group D3 compared with group C (P<0.05).CONCLUSION: PGE2 can induce myocardial hypertrophy of the cultured H9c2 cardiac cells in a dose-dependent manner. Larger dose of PGE2 stimulate pathological myocardial hypertrophy. Inhibition of PGE2 is expected to be a new target to control myocardial hypertrophy.

Key words: Prostaglandin E2, Cycloxygenase, Myocardial hypertrophy