复方山菊对自发性高血压大鼠主动脉损伤的保护作用及机制

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (7): 732-737.

复方山菊对自发性高血压大鼠主动脉损伤的保护作用及机制

刘艳, 杨解人, 郝伟, 张俊秀, 唐丽娟, 陈国祥

皖南医学院药理学教研室,芜湖 241002,安徽

收稿日期:2012-09-06 修回日期:2012-12-22 出版日期:2013-07-26 发布日期:2013-06-20
通讯作者: 杨解人, 女, 教授,研究方向: 临床药理学。Tel: 13955382763 E-mail: jryang1955@sina.com
作者简介:刘艳,女,硕士研究生,研究方向:临床药理学。Tel: 13855366432 E-mail: liuyanemail@yahoo.com

Mechanisms and protective effect of compound Shanju on aortal injury in spontaneously hypertensive rats

LIU Yan, YANG Jie-ren, HAO Wei, ZHANG Jun-xiu, TANG Li-juan, CHEN Guo-xiang

Department of Pharmacology, Wannan Medical College, Wuhu 241002, Anhui, China

Received:2012-09-06 Revised:2012-12-22 Online:2013-07-26 Published:2013-06-20

摘要/Abstract

摘要： 目的: 探讨复方山菊对自发性高血压大鼠(SHR)主动脉的保护作用及可能机制。方法: SHR 35只,随机分为SHR(蒸馏水 5 mL·kg^-1·d^-1,n=7)模型组、复方山菊高、中、低(0.45、0.15、0.05 g·kg^-1·d^-1,n=7)剂量组、卡托普利(0.03 g·kg^-1·d^-1,n=7)阳性对照组,另设WKY(蒸馏水 5 mL·kg^-1·d^-1,n=7)阴性对照组,连续灌胃给药16周,每天1次。尾袖法每两周测定大鼠动脉血压。16周后,HE染色观察主动脉病理组织学变化;Masson染色观察主动脉胶原纤维变化;免疫组化法观察主动脉内皮型一氧化氮合成酶(eNOS)、转化生长因子-β₁(TGF-β₁)蛋白的表达。结果: 随着复方山菊剂量的增加, 主动脉的病理损伤得到不同程度的改善,主动脉中膜胶原面积逐渐减小,胶原纤维沉积逐渐减轻;主动脉TGF-β₁蛋白表达逐渐减少,eNOS蛋白表达逐渐增加。结论: 复方山菊可明显减轻主动脉的病理损伤,减少主动脉胶原纤维沉积,下调TGF-β₁及上调eNOS蛋白的表达,对SHR主动脉具有一定的保护作用。

关键词: 复方山菊, 自发性高血压大鼠, 胶原沉积, 转化生长因子-β₁, 内皮型一氧化氮合成酶

Abstract: AIM: To observe the protective effect of compound Shanju on the aortal injury in spontaneously hypertensive rats (SHRs) and explore its conceivable mechanisms.METHODS: SHRs (35) were randomly divided into SHR model (distilled water 5 mL·kg^-1·d^-1,n=7) group, compound Shanju with high, medium and low dosages (0.45, 0.15, and 0.05 g·kg^-1·d^-1,n=7, respectively) groups, and captopril (0.03 g·kg^-1·d^-1,n=7) positive control group. In addition, the WKY (distilled water 5 mL·kg^-1·d^-1,n=7) negative control group was established. All animals were administrated for 16 weeks. Blood pressure was measured by tail cuff for 16 weeks fortnightly interval with all animals. After 16 weeks, the primary pathologic changes of aorta were observed by HE staining, and collagen deposition was stained by Masson visually. The expression of eNOS and TGF-β₁ protein were measured by immunohistochemistry.RESULTS: With the increase dose of compound Shanju, the aortal pathological damage had been improved, the area of collage deposition had been ameliorated gradually in aortal tunica media. The expression of TGF-β₁ protein downregulated and that of eNOS protein upregulated inch by inch.CONCLUSION: Compound Shanju can significantly ameliorate the aortal injury and collagen deposition, suggesting this medicine may protect the aorta from injury through downregulating TGF-β₁ and upregulating eNOS protein expression.

Key words: Compound Shanju, Spontaneously hypertensive rats, Collagen deposition, TGF-β₁, eNOS

中图分类号:

R965.2

刘艳, 杨解人, 郝伟, 张俊秀, 唐丽娟, 陈国祥. 复方山菊对自发性高血压大鼠主动脉损伤的保护作用及机制[J]. 中国临床药理学与治疗学, 2013, 18(7): 732-737.

LIU Yan, YANG Jie-ren, HAO Wei, ZHANG Jun-xiu, TANG Li-juan, CHEN Guo-xiang. Mechanisms and protective effect of compound Shanju on aortal injury in spontaneously hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(7): 732-737.

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