孤束核微注射内皮素-1(1-31)对心血管活动的影响

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (7): 754-759.

孤束核微注射内皮素-1(1-31)对心血管活动的影响

李培武^1,2, 王先坤², 种晓琴³, 李培杰², 崔鸿斌², 鲁彦⁴, 傅仲学¹

¹重庆医科大学附属第一医院普外科,重庆 400030;
²兰州大学第二医院急诊科,兰州 730050,甘肃;
³解放军第一医院高压氧神经内科;
⁴检验科,兰州 730030,甘肃

收稿日期:2012-11-01 修回日期:2013-05-20 出版日期:2013-07-26 发布日期:2013-06-20
通讯作者: 傅仲学,男,教授,博士生导师,研究方向:胃肠道肿瘤及腹部微创外科技术。Tel: 023-67706399 E-mail: lu73free@yahoo.com.cn
作者简介:李培武,男,副主任医师,研究方向:普外科与临床药理。Tel: 0931-8942031 E-mail: lipw360@yahoo.com.cn
基金资助:
甘肃省技术研究与开发专项计划项目(0709TCYA062)

Cardiovascular responses evoked by endothelin-1(1-31) microinjected into the nucleus tractus solitarius

LI Pei-wu^1,2, WANG Xian-kun², CHONG Xiao-qin³, LI Pei-jie², CUI Hong-bin², LU Yan⁴, FU Zhong-xue¹

¹Department of General Surgeon, First Affiliated Hospital of Chongqing Medical University; Chongqing 40030,China;
²Emergency Department of Second Hospital Affiliated to University; Lanzhou 730050,Gansu,China;
³Department of Hyperbaric Oxygen and Neurology;
⁴Department of Clinical Laboratory, First Hospital of the People's Liberation Army, Lanzhou 730030,Gansu,China

Received:2012-11-01 Revised:2013-05-20 Online:2013-07-26 Published:2013-06-20

摘要/Abstract

摘要： 目的: 观察内皮素-1(1-31)[ET-1(1-31)] 在大鼠孤束核(Nucleus tracts solitarius, NTS)产生的心血管效应,并探讨其作用机制。方法: 雄性SD大鼠90只,其中50只随机分为双侧NTS注射组、单侧NTS注射组和人工脑脊液(aCSF)组,分别在双侧或单侧NTS微量注射ET-1(1-31) (0.5~2.0 pmoL)或aCSF (100 nL),观察ET-1(1-31) 在NTS内的心血管效应。11只大鼠观察ET-1(1-31) (1.0 pmoL)在NTS水平对动脉压力反射功能(BRS)产生的影响。其余29只大鼠分别预先给予ET_A受体拮抗剂BQ₁₂₃、ET_B受体拮抗剂BQ₇₈₈、非选择性谷氨酸受体拮抗剂犬尿烯酸(KYN)或对照(aCSF),探讨ET-1(1-31)在大鼠NTS内产生的心血管效应机制。结果: 在大鼠NTS双侧或单侧微量注射ET-1(1-31)剂量依赖性降低大鼠血压并减缓心率;NTS双侧微注射ET-1(1-31)(1 pmoL) 显著减弱大鼠BRS(P<0.05);ET_A受体拮抗剂BQ₁₂₃ 或KYN显著减弱单侧NTS微量注射ET-1(1-31)(1.0 pmoL)产生的降低血压和减缓心率作用(P<0.05);单侧NTS注射ET_B受体拮抗剂BQ₇₈₈对NTS微量注射ET-1(1-31)(1.0 pmoL)产生的降低血压和减缓心率作用没有显著的影响(P>0.05)。结论: NTS微量注射ET-1(1-31)能够降低麻醉大鼠的血压并减缓心率,其作用可能是由ET_A受体和谷氨酸受体所介导。

关键词: 内皮素-1(1-31), 孤束核, 心血管, 压力反射

Abstract: AIM: To observe the cardiovascular functions of endothelin-1(1-31)[ ET-1(1-31)] within the nucleus tract solitarius(nucleus tracts solitarius, NTS) and explore their mechanism.METHODS: 90 male SD rats were included into the present study. Among them, 50 were randomized into bilateral or unilateral microinjection of ET-1(1-31)(0.5-2.0 pmoL)or aCSF (100 nL) group, in which ET-1(1-31) (0.5-2.0 pmoL) or aCSF (100 nL) was bilaterally or unilaterally applied to observe the dose-dependant responses of ET-1(1-31) within the NTS. ET-1(1-31) (1.0 pmol) or aCSF (100 nL) was bilaterally applied into the NTS to observe the influences of ET-1(1-31) (1.0 pmoL) on the arterial baroreflex function (BRS) within the bilateral NTS in 11 rats. ET_A receptor antagonist BQ₁₂₃, ET_B receptor antagonist BQ₇₈₈or non-selective glutamate receptor antagonist kynurenine (KYN) was prior applied to explore the mechanism of ET-1(1-31) within the NTS in 29 rats.RESULTS: Bilateral or unilateral microinjection of ET-1(1-31) into the NTS produced dose-dependant hypotension and bradycardia of rats.Bilateral microinjection of ET-1(1-31) into the NTS significantly decreased the BRS function.Pretreatment with BQ₁₂₃ or KYN significantly decreased BP and HR responses induced by unilateral microinjection of ET-1(1-31) (1.0 pmol) into the NTS. There was no obvious influence to BP and HR responses treatment with BQ₇₈₈.CONCLUSION: ET-1(1-31) within the NTS produces hypotension and bradycardia, mediated by ET_A receptors and glutamate receptor at least partly.

Key words: Endothelin-1(1-31), Nucleus tract solitarius, Cardiovascular, Baroreflex

中图分类号:

R965.2

李培武, 王先坤, 种晓琴, 李培杰, 崔鸿斌, 鲁彦, 傅仲学. 孤束核微注射内皮素-1(1-31)对心血管活动的影响[J]. 中国临床药理学与治疗学, 2013, 18(7): 754-759.

LI Pei-wu, WANG Xian-kun, CHONG Xiao-qin, LI Pei-jie, CUI Hong-bin, LU Yan, FU Zhong-xue. Cardiovascular responses evoked by endothelin-1(1-31) microinjected into the nucleus tractus solitarius[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(7): 754-759.

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