国产齐考诺肽对L5脊神经结扎后大鼠神经病理性疼痛的疗效

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (7): 760-763.

国产齐考诺肽对L5脊神经结扎后大鼠神经病理性疼痛的疗效

邓丽珍¹, 屠伟峰¹, 马亚平², 尹睛¹, 杨学敏², 康旭², 聂涛²

¹南方医科大学附属广州军区广州总医院麻醉科、全军临床麻醉中心,广州 510010,广东;
²深圳翰宇药业股份有限公司、多肽药物国家地方联合工程实验室,深圳 518057,广东

收稿日期:2013-01-17 修回日期:2013-06-05 出版日期:2013-07-26 发布日期:2013-06-20
通讯作者: 屠伟峰,男,主任医师,博士生导师,研究方向:慢性疼痛的防治及机制。Tel: 13922116606 E-mail: wftuyx02@163.com
作者简介:邓丽珍,在读研究生,主治医师,研究方向:慢性疼痛的防治及机制。Tel: 18142890808 E-mail: denglz2010@163.com
基金资助:
广东省科技计划项目(2012A080202012)

Efficacy of domestic Ziconotide in treating neuropathic pain induced by L5 spinal nerve ligation in rats

DENG Li-zhen¹, TU Wei-feng¹, MA Ya-ping² , YING Qin¹, YANG Xue-min², KANG Xu², NIE Tao²

¹Department of Anesthesiology, Guangzhou General Hospital of Guangzhou Military Command Affiliated to Nanfang Medical University, Guangzhou 510515,Guangdong, China;
²State & Local Joint Engineering Laboratory of Peptide Drug, Shenzhen 518057,Guangdong, China

Received:2013-01-17 Revised:2013-06-05 Online:2013-07-26 Published:2013-06-20

摘要/Abstract

摘要： 目的: 观察国产齐考诺肽注射液对大鼠L5脊神经结扎后神经病理性疼痛(L5SNL)的镇痛作用。方法: 取雄性SD大鼠共40只,随机分为5组(n=8),包括假手术组(SC组):0.9%NS 20 μL、L5SNL模型组(L5SNL组):0.9%NS 20 μL;DZ1组:第一批次国产齐考诺肽 100 ng;DZ2组:第二批次国产齐考诺肽 100 ng;IZ组:进口齐考诺肽 100 ng。采用测试50%机械刺激撤足阈值(50%PWT)和热刺激撤足潜伏值(TWD)的方法,观察大鼠治疗前后机械痛阈和热痛阈的变化,并计算药物的最大效应百分比(MPE),评价抗神经病理性疼痛的效果。结果: 与L5SNL模型组相比,鞘内单次给予国产及进口齐考诺肽 100 ng 均可显著提高大鼠50%PWT和TWD(P＜0.01);与IZ组相比,国产齐考诺肽组的MPE和TWD均无统计学差异(P＞0.05)。结论: 国产齐考诺肽对L5脊神经结扎后大鼠神经病理性疼痛具有显著的疗效。

关键词: 齐考诺肽, 神经病理性疼痛, 50%机械刺激撤足阈值, 热刺激撤足潜伏值, 最大效应百分比

Abstract: AIM: To investigate the efficacy of domestic Ziconotide in treating neuropathic pain induced by L5 spinal nerve ligation in rats.METHODS: Forty male SD rats (weighted from180 to 220 g) were randomly divided into five groups, including sham control group(Group SC): 0.9%NS 20 μL; L5SNL model control group (Group L5SNL): 0.9%NS 20 μL; DZ1 group: the first batch of domestic ziconotide 100 ng; DZ2 group: the second batch of domestic ziconotide 100 ng; IZ group: imported ziconotide 100 ng. The changes of the mechanical pain threshold and thermal pain threshold in these rats by testing their 50% paw withdrawal threshold(50%PWT) and thermal withdrawal duration(TWD) pain threshold in these rats were observed, and the maximum possible effect (MPE) was calculated to evaluate the analgetic efficacy of domestic and imported ziconotide in relieving the neuropathic pain.RESULTS: Compared with the model group, both 50%PWT and TWD were significantly increased by intrathecal injection 100 ng of domestic Ziconotide and imported one respectively(P＜0.01); There were no significant differences in the maximum possible effect(MPE) of inhibiting the mechanical pain and TWD between the domestic Ziconotide group and imported one.CONCLUSION: Domestic Ziconotide has a significant effect on the neuropathic pain induced by L5 spinal nerve ligation in rats.

Key words: Ziconotide, Neuropathic pain, 50% paw withdrawal threshold, Thermal withdrawal duration, The maximum possible effect

中图分类号:

R965.2

邓丽珍, 屠伟峰, 马亚平, 尹睛, 杨学敏, 康旭, 聂涛. 国产齐考诺肽对L5脊神经结扎后大鼠神经病理性疼痛的疗效[J]. 中国临床药理学与治疗学, 2013, 18(7): 760-763.

DENG Li-zhen, TU Wei-feng, MA Ya-ping , YING Qin, YANG Xue-min, KANG Xu, NIE Tao. Efficacy of domestic Ziconotide in treating neuropathic pain induced by L5 spinal nerve ligation in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(7): 760-763.

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