大鼠抑郁行为的性别差异与单胺转运体及单胺氧化酶B关系的实验研究

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (9): 993-998.

大鼠抑郁行为的性别差异与单胺转运体及单胺氧化酶B关系的实验研究

汪丽佳, 秦丽娟, 刘丹, 刘任, 周岐新

重庆医科大学药理教研室,重庆市生物化学与分子药理学实验室,重庆 400000

收稿日期:2013-03-21 修回日期:2013-06-28 发布日期:2013-09-07
通讯作者: 周岐新,男,博士生导师,研究方向:神经药理学。Tel: 13527534910 E-mail: cqzhouqx@yahoo.com.cn
作者简介:汪丽佳,女,硕士研究生,研究方向:神经药理学。Tel: 18725768027 E-mail: 492759231@qq.com

The correlation between sex difference of depressive behavior and expression of monoamine transporter and MAO-B in rats

WANG Li-jia, QIN Li-juan, LIU Dan, LIU Ren, ZHOU Qi-xin

Department of Pharmacology, Chongqing Medical University, Department of Biochemistry and Molecular Pharmacology, Chongqing 400000, China

Received:2013-03-21 Revised:2013-06-28 Published:2013-09-07

摘要/Abstract

摘要： 目的: 探讨慢性轻度不可预见刺激(CUMS)致大鼠抑郁行为的性别差异与神经细胞单胺氧化酶B(MAO-B)、5-羟色胺转运体(5-HTT)及去甲肾上腺素转运体(NET)表达的关系。方法: SD大鼠雌雄各30只,随机分为雄性对照组(MCG)、雄性模型组(MMG)、雌性对照组(FCG)和雌性模型组(FMG),采用孤养结合CUMS方式建立大鼠抑郁模型。以开场实验和强迫游泳实验评价大鼠焦虑抑郁行为;Western blot法和荧光定量PCR法分别测定脑桥NET和海马5-HTT及MAO-B蛋白和mRNA表达。结果: 经CUMS处理的MMG大鼠开场实验水平和垂直得分均显著低于MCG,FMG大鼠垂直得分明显低于FCG,且MMG大鼠水平和垂直得分也显著低于FMG;FMG和MMG大鼠强迫游泳不动时间显著长于各自对照组,且MMG大鼠不动时间显著长于FMG;与各自对照组相比,FMG和MMG大鼠5-HTT蛋白表达均明显降低,而MAO-B表达明显升高;NET蛋白表达仅MMG明显升高,且显著高于FMG大鼠。结论: CUMS更易诱导雄性大鼠产生抑郁样行为,其原因可能与CUMS导致两性大鼠MAO-B、NET、5-HTT表达量的差异有关。

关键词: 抑郁症, 性别差异, 去甲肾上腺素转运体, 5-羟色胺转运体, 单胺氧化酶B

Abstract: AIM: To investigate the relationship between sex difference of CUMS-induced depressive behaviors of rats and the expressions of monoamine oxidase B (MAO-B), 5-hydroxytryptamine transporter (5-HTT) and norepinephrine transporter (NET).METHODS: 60 SD rats (30 for each male and female) were randomly divided into following groups:male control group (MCG),male model group (MMG),female control group (FCG) and female model group (FMG). Chronically unpredicted mild stress (CUMS) with solitary condition was taken to establish rat depression model. The open-field test and forced swimming test were used to evaluate the behavioral changes of experimental rats. The expressions of MAO-B and 5-HTT in hippocampus and NET in pons were determined by Western blot (WB) and Real-time fluorescent quantitative PCR, respectively.RESULTS: Significantly decreasing locomotor activity in open-field test and increasing immobility time in forced swimming test in MMG and FMG with remarkable difference between MMG and FMG were observed, compared with corresponding control groups. Expressions of obviously decreased 5-HTT and elevated MAO-B and NET also were found in both model groups with significant sex differences.CONCLUSION: Depression model is much easier to be induced in male rats than female by CUMS, which may be at least partly related to the different expressions of MAO-B, 5-HTT and NET.

Key words: Depression, Sex difference, NET, 5-HTT, MAO-B

中图分类号:

R965.2

汪丽佳, 秦丽娟, 刘丹, 刘任, 周岐新. 大鼠抑郁行为的性别差异与单胺转运体及单胺氧化酶B关系的实验研究[J]. 中国临床药理学与治疗学, 2013, 18(9): 993-998.

WANG Li-jia, QIN Li-juan, LIU Dan, LIU Ren, ZHOU Qi-xin. The correlation between sex difference of depressive behavior and expression of monoamine transporter and MAO-B in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(9): 993-998.

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