去甲肾上腺素对人肺癌细胞株H460增殖、迁移和侵袭能力的影响

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (11): 1217-1221.

去甲肾上腺素对人肺癌细胞株H460增殖、迁移和侵袭能力的影响

龙晓莉¹, 张遥衡¹, 卢卫琴¹, 凌泽毅²

¹ 重庆医科大学附属永川医院老年内科,
² 耳鼻喉科,重庆402160

收稿日期:2014-01-30 修回日期:2014-08-21 出版日期:2014-11-26 发布日期:2014-12-09
通讯作者: 凌泽毅,男,主治医生, 研究方向:耳鼻喉临床研究。E-mail: 2664290086@qq.com
作者简介:龙晓莉,女,本科,主治医生,研究方向:老年呼吸性疾病。 Tel: 18696604900 E-mail: jy02024700@163.com

Effects of norepinephrine on proliferation, migration and invasion of human lung carcinoma cell line H460

LONG Xiao-li¹, ZHANG Yao-heng¹, LU Wei-qin¹, LING Ze-yi²

¹ Department of Geriatrics;
² Department of Otorhinolaryngology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402160, China

Received:2014-01-30 Revised:2014-08-21 Online:2014-11-26 Published:2014-12-09

摘要/Abstract

摘要： 目的研究去甲肾上腺素(norepinephrine,NE)对人肺癌细胞株H460增殖、迁移和侵袭能力的影响。方法体外培养人肺癌细胞株H460,用NE干预后,CCK-8法检测细胞增殖,划痕愈合实验评价细胞迁移能力,侵袭小室实验观察细胞侵袭力,Western Blotting法检测细胞p38MAPK磷酸化蛋白(Phos-p38)的表达。结果 10 μmol/L NE可显著促进H460细胞增殖,

关键词: 去甲肾上腺素, H460细胞, 迁移, 增殖, 侵袭

Abstract: AIM: To investigate the effect of norepinephrine (NE) on proliferation, migration and invasion of human lung cancer cell line H460. METHODS: Human lung cancer cell line H460 were cultured in vitro and treated with NE, then CCK-8 mehod, scratch wound healing assay and transwell assay were applied to measure cell proliferation, migration and invasion. The expression of p38MAPK phosphorylation (Phos-p38) were examined by Western blotting. RESULTS: Compared with the control, 10 μmol/L NE significantly enhanced proliferation, migration and invasion of human lung cancer cell line H460, and elevated p38MAPK phosphorylation level (P<0.01). These effects were completely reversed by β-adrenergic receptor (β-AR) antagonist propranolol (PRO) or p38MAPK inhibitor U0126. CONCLUSION: NE could stimulate lung cancer cell proliferation, migration and invasion, its mechanism may be related with activation of P38MAPK pathway.

Key words: norepinephrine, lung cancer, proliferation, migration, invasion

中图分类号:

R965.2

龙晓莉, 张遥衡, 卢卫琴, 凌泽毅. 去甲肾上腺素对人肺癌细胞株H460增殖、迁移和侵袭能力的影响[J]. 中国临床药理学与治疗学, 2014, 19(11): 1217-1221.

LONG Xiao-li, ZHANG Yao-heng, LU Wei-qin, LING Ze-yi. Effects of norepinephrine on proliferation, migration and invasion of human lung carcinoma cell line H460[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(11): 1217-1221.

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