水合氯醛诱发RAW264.7巨噬细胞凋亡及其机制

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (2): 121-126.

• 基础研究 • 下一篇

水合氯醛诱发RAW264.7巨噬细胞凋亡及其机制

潘庆军¹, 朱学芝², 刘渊³

¹广东医学院附属医院,湛江 524001,广东;
²广东粤海饲料集团有限公司,湛江 524017,广东;
³香港大学李嘉诚医学院,香港 999077

收稿日期:2013-04-08 修回日期:2013-08-29 出版日期:2014-02-26 发布日期:2014-03-31
作者简介:潘庆军,男,博士,讲师,主要研究抗感染免疫和自身免疫疾病。Tel: 0759-2387164 E-mail: stilwapan@gmail.com
基金资助:
国家自然科学基金资助项目(81202346);广东省自然科学基金(S2012040006216);广东省卫生厅基金(B2012284);湛江市科技攻关项目(2012C3101028/2013B01086);广东医学院和附属医院博士启动项目资助

Apoptosis of RAW264.7 macrophages induced by chloral hydrate and its mechanisms

PAN Qing-jun¹, ZHU Xue-zhi², LIU Yuan³

¹Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong, China;
²Guangdong Yuehai Feed Group Co., Ltd., Zhanjiang 524017, Guangdong, China;
³the University of Hong Kong, Li Ka Shing Faculty of Medicine, Hongkong 999077, China

Received:2013-04-08 Revised:2013-08-29 Online:2014-02-26 Published:2014-03-31

摘要/Abstract

摘要： 目的: 探讨水合氯醛处理对RAW264.7巨噬细胞凋亡的影响及其机制。方法: 以不同浓度的水合氯醛对RAW264.7巨噬细胞处理不同时间,采用形态学观察、Annexin V-FITC/PI双染细胞凋亡检测试剂、Hochest33258染色和DNA ladder试剂检测RAW264.7巨噬细胞的凋亡情况,并检测Fas/FasL表达情况。结果: 水合氯醛处理后RAW264.7巨噬细胞形态由梭形变圆直至脱落悬浮;Annexin V-FITC/PI双染检测显示水合氯醛处理可诱导RAW264.7巨噬细胞从早期向晚期凋亡,Hochest33258染色和DNA ladder检测均显示诱导凋亡;同时高表达Fas,但不表达FasL。结论: 水合氯醛可通过Fas/FasL途径诱导RAW264.7巨噬细胞细胞凋亡。

关键词: 水合氯醛, RAW264.7巨噬细胞, 凋亡, Fas/FasL

Abstract: AIM: To investigate the effect of chloral hydrate treatment on the apoptosis of RAW264.7 macrophages and the mechanisms of apoptosis.METHODS: To treat RAW264.7 macrophages by chloral hydrate with different concentrations and different times, then to observe the morphological changes. Also, Annexin V-FITC/PI double staining assay, Hochest 33258 and and DNA ladder assay were used to detect the apoptosis, and the expression of Fas/FasL.RESULTS: After treatment with chloral hydrate, the morphology of RAW264.7 macrophages changed from fusiform to round, and suspension at last. Annexin V-FITC/PI double staining assay showed RAW264.7 macrophages were from early to late apoptosis after chloral hydrate treatment. Hochest 33258 and and DNA ladder assay showed chloral hydrate treatment can induced the apoptosis of RAW264.7 macrophages. Also, the apoptotic RAW264.7 macrophages expressed higher levels of Fas, but did not express FasL.CONCLUSION: Chloral hydrate treatment could induce RAW264.7 macrophages apoptosis via the Fas/FasL pathway.

Key words: chloral hydrate, RAW264.7 macrophages, apoptosis, Fas/FasL

中图分类号:

R965.2

潘庆军, 朱学芝, 刘渊. 水合氯醛诱发RAW264.7巨噬细胞凋亡及其机制[J]. 中国临床药理学与治疗学, 2014, 19(2): 121-126.

PAN Qing-jun, ZHU Xue-zhi, LIU Yuan. Apoptosis of RAW264.7 macrophages induced by chloral hydrate and its mechanisms[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(2): 121-126.

参考文献

[1] Mellon RD, Simone AF, Rappaport BA. Use of anesthetic agents in neonates and young children [J]. Anesth Analg, 2007, 104(3): 509-520.
[2] Sury MR, Fairweather K. The effect of melatonin on sedation of children undergoing magnetic resonance imaging [J]. Br J Anaesth, 2006, 97(2): 220-225.
[3] Harrison D, Loughnan P, Manias E, et al. Utilization of analgesics, sedatives, and pain scores in infants with a prolonged hospitalization: a prospective descriptive cohort study [J]. Int J Nurs Stud, 2009, 46(5): 624-632.
[4] Layangool T, Sangtawesin C, Kirawittaya T, et al. A comparison of oral chloral hydrate and sublingual midazolam sedation for echocardiogram in children [J]. J Med Assoc Thai, 2008, 91 (Suppl 3): S45-52.
[5] Liu N, Fan GG, Yu B, et al. Effect of slight sedation with chloral hydrate on the BOLD response of children s visual cortex: a self-control fMRI study [J]. Chin J Med Imaging Technol, 2008, 24(12): 1901-1904.
[6] 潘庆军,刘渊. 麻醉药的抗炎抗感染功效及其作用靶点研究进展[J]. 中国临床药理学与治疗学, 2012, 17(8): 940-943.
[7] Zhu M, Ackerman JJ, Yablonskiy DA. Body and brain temperature coupling: the critical role of cerebral blood flow [J]. J Comp Physiol B, 2009, 179(6): 701-710.
[8] Uematsu M, Takasawa M, Hosoi R, et al. Uncoupling of flow and metabolism by chloral hydrate: a rat in-vivo autoradiographic study [J]. Neuroreport, 2009, 20(3): 219-222.
[9] Yuksel BC, Serdar SE, Tuncel A, et al. Effect of tempol, a membrane-permeable radical scavenger, on mesenteric blood flow and organ injury in a murine cecal ligation and puncture model of septic shock [J]. Eur Surg Res, 2009, 43(2): 219-227.
[10] Pan Q, Liu Y, Zheng J, et al. Protective effect of chloral hydrate against lipopolysaccharide/ D-galactosamine-induced acute lethal liver injury and zymosan-induced peritonitis in mice [J]. Int Immunopharmacol, 2010, 10(8): 967-977.
[11] Haslett C, Savill J. Why is apoptosis important to clinicians [J] ? BMJ, 2001, 322(7301): 1499-1500.
[12] Medina-Gomez G, Vidal-Puig A. Adipose tissue as a therapeutic target in obesity [J]. Endocrinol Nutr, 2009, 56(8): 404-411.
[13] Griffith TS, Brunner T, Fletcher SM, et al. Fas ligand-induced apoptosis as a mechanism of immune privilege [J]. Science, 1995, 270 (5239): 1189-1192.
[14] Tabas I. Macrophage death and defective inflammation resolution in atherosclerosis [J]. Nat Rev Immunol, 2010, 10(1): 36-46.
[15] 李仪奎. 中药血清药理学实验方法的若干问题[J]. 中药新药与临床药理, 1999, 10(2): 95-98.
[16] Fukuyama H, Adachi M, Suematsu S, et al. Requirement of Fas expression in B cells for tolerance induction [J]. Eur J Immunol, 2002, 32(1): 223-230.
[17] Rothstein TL. Inducible resistance to Fas-mediated apoptosis in B cells [J]. Cell Res, 2000, 10(4): 245-266.
[18] Tsutsui H, Kayagaki N, Kuida K, et al. Caspase-1-independent, Fas/Fas ligand-mediated IL-18 secretion from macrophages causes acute liver injury in mice [J]. Immunity, 1999, 11(3): 359-367.
[19] Hohlbaum AM, Gregory MS, Ju ST, et al. Fas ligand engagement of resident peritoneal macrophages in vivo induces apoptosis and the production of neutrophil chemotactic factors [J]. J Immunol, 2001, 167(11): 6217-6224.
[20] Li HL, Wang AB, Zhang R, et al. A20 inhibits oxidized low-density lipoprotein-induced apoptosis through negative Fas/Fas ligand-dependent activation of caspase-8 and mitochondrial pathways in murine RAW264.7 macrophages [J]. J Cell Physiol, 2006, 208(2): 307-318.
[21] Fukui M, Imamura R, Umemura M. et al. Pathogen-associated molecular patterns sensitize macrophages to Fas ligand-induced apoptosis and IL-1 beta release [J]. J Immunol, 2003, 171(4): 1868-1874.

水合氯醛诱发RAW264.7巨噬细胞凋亡及其机制

Apoptosis of RAW264.7 macrophages induced by chloral hydrate and its mechanisms

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	范杰, 金永明, 江孝龙, 蒋国华. lncRNA HOTAIR调控miR-206影响类风湿关节炎滑膜细胞增殖和凋亡的分子机制研究[J]. 中国临床药理学与治疗学, 2023, 28(7): 736-742.
[2]	杨晓佳, 江萌, 吴敏, 张伊黎, 吕兰, 吴嫄芬, 王鑫昱, 刘立权. 藏红花素介导DKK3调控GSK-3β/β-catenin通路对阿尔兹海默症大鼠的认知改善作用[J]. 中国临床药理学与治疗学, 2023, 28(5): 489-497.
[3]	侯小煜, 冷玉芳, 曹雪芬, 吕兴娇, 韩晓霞, Janvier NIBARUTA, 刘永强. 五味子乙素减轻小鼠肠缺血再灌注损伤的网络药理学分析及实验验证[J]. 中国临床药理学与治疗学, 2023, 28(2): 147-154.
[4]	王剑, 孙永东, 周兴玮, 刘雷, 陈龙, 童兴科, 朱佳丽. 黄芩素经由miR-125b-5p/IRF4轴进而促进喉癌细胞死亡并抑制其侵袭的机制研究[J]. 中国临床药理学与治疗学, 2023, 28(11): 1209-1218.
[5]	朱瑞芳, 郭东凯, 智慧, 江翊国, 张悦翎, 钱晓萍, 季士亮. ROS-NF-κB-p38MAPK通路探索榄香烯联合硼替佐米抗多发性骨髓瘤的机制研究[J]. 中国临床药理学与治疗学, 2023, 28(11): 1219-1226.
[6]	周欢, 徐铭, 李波, 刘春英, 王淳. PI3K/Akt/FOXO3a信号通路介导的保护性自噬参与肺腺癌获得性顺铂耐药表型重塑[J]. 中国临床药理学与治疗学, 2022, 27(9): 961-970.
[7]	华海燕, 严杰, 秦宇芬. 五味子乙素通过抑制心肌细胞凋亡减轻大鼠心肌缺血再灌注损伤的实验研究[J]. 中国临床药理学与治疗学, 2022, 27(8): 848-856.
[8]	李雪恒, 李龙. 褪黑素与特发性肺纤维化关系的研究进展[J]. 中国临床药理学与治疗学, 2022, 27(6): 715-720.
[9]	杨柳, 罗爱林, 李世勇. 全麻药物损伤发育期大脑的研究进展[J]. 中国临床药理学与治疗学, 2022, 27(12): 1381-1390.
[10]	谭峰浪, 任宏丽, 王宏飞. 黄柏提取物对Aβ_1-42诱导的海马神经细胞的影响[J]. 中国临床药理学与治疗学, 2022, 27(11): 1213-1220.
[11]	潘蓉蓉, 支英豪, 金永喜, 周夏慧. 茶黄素调节CaMKK2/AMPK信号通路对脑出血大鼠神经元凋亡和血脑屏障的影响[J]. 中国临床药理学与治疗学, 2022, 27(11): 1240-1246.
[12]	李莹, 姚伟, 王萌, 余志宏, 龚园其, 蓝海兵, 齐协飞. 鸢尾素通过NF-κB和JNK信号通路减轻屋尘螨诱导的气道上皮细胞炎症及凋亡的实验研究[J]. 中国临床药理学与治疗学, 2022, 27(10): 1106-1112.
[13]	刘云帆, 杜月梅, 刘晓艺, 高丽萍. 枸杞多糖对顺铂诱导小鼠睾丸支持细胞凋亡的影响[J]. 中国临床药理学与治疗学, 2021, 26(6): 624-630.
[14]	魏松长, 杨红琪, 张宏娟, 施晓丽, 任凯文. RON蛋白与CXC趋化因子受体4蛋白的表达与去势抵抗型前列腺癌患者阿比特龙耐药的相关性[J]. 中国临床药理学与治疗学, 2021, 26(4): 414-422.
[15]	张伟萍, 江建军, 陈晓锋, 许莎莎. 栀子苷通过抑制VPO1/ERK1/2信号通路减轻糖尿病心肌病大鼠的心肌细胞凋亡[J]. 中国临床药理学与治疗学, 2021, 26(2): 129-136.