β3肾上腺素能受体激动剂对离体人肺脏肺泡液体清除的作用

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (3): 271-274.

β₃肾上腺素能受体激动剂对离体人肺脏肺泡液体清除的作用

李乃静¹, 李伟², 谷秀³, 李岩¹, 何平¹

¹中国医科大学附属盛京医院老年病科,沈阳 110004,辽宁;
²沈阳药科大学药物分析教研室,沈阳 110004,辽宁;
³中国医科大学附属盛京医院呼吸内科,沈阳 110004,辽宁

收稿日期:2013-03-11 修回日期:2014-03-07 出版日期:2014-03-26 发布日期:2014-04-10
作者简介:李乃静,女,博士,副教授,主要从事老年呼吸系统疾病的研究。Tel: 18940257247 E-mail: lnjlw2003@163.com
基金资助:
辽宁省科学技术计划项目(201202245);教育部留学回国人员科研启动基金(教外司留^[2011]1139)

Effect of CGP-12177 on alveolar fluid clearance in isolated human lung

LI Nai-jing¹, LI Wei², GU Xiu³, LI Yan¹, HE Ping¹

¹Department of Gerontology, the Shengjing Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning, China;
²College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110004, Liaoning, China;
³Department of Respiratory Medicine, the Shengjing Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning, China

Received:2013-03-11 Revised:2014-03-07 Online:2014-03-26 Published:2014-04-10

摘要/Abstract

摘要： 目的: 制备离体人肺脏模型,探讨β₃肾上腺素能受体激动剂CGP-12177对肺泡液体清除率(AFC)的作用及其机制。方法: 以肺泡内Evans蓝标记的白蛋白浓度的变化测定AFC。首先将 10^-4 mol/L 到 10^-7 mol/L 的CGP-12177分别灌注到离体人肺脏肺泡腔内,测定AFC的变化。然后将 10^-5 mol/L CGP-12177分别与α受体阻滞剂酚妥拉明、β₁受体阻滞剂阿替洛尔、β₂受体阻滞剂ICI-118551、β₃受体阻滞剂SR59230A、钠通道阻断剂氨氯吡咪、Na⁺-K⁺-ATP酶阻断剂哇巴因混合后灌注到离体人肺脏肺泡腔内,测定AFC的变化。结果: 10^-7 mol/L 和 10^-4 mol/L CGP-12177对离体人肺脏肺泡液体清除率没有影响,10^-6 mol/L 和10^-5 mol/L 的CGP-12177能够显著提高离体人肺脏肺泡液体清除率。CGP-12177增加离体人肺脏AFC的作用能够被SR59230A、氨氯吡咪和哇巴因抑制。结论: 高选择性CGP-12177主要通过调节Na⁺-K⁺-ATP酶的活性提高离体人肺脏肺泡液体清除能力。

关键词: 离体人肺脏, 肾上腺素能受体激动剂, 肺水肿

Abstract: AIM: To establish the model of isolated human lung, and to study the effect of CGP-12177 on alveolar fluid clearance (AFC) in isolated human lung and the mechanisms involved.METHODS: The AFC was measured by the concentration of Evansblue-labeled album in the istilled and aspirated solution. Isomolar albumin solution in the presence of 10^-4 mol/L to 10^-7 mol/L CGP-12177 (selective β₃-adrenergic agonists) was injected into the alveolar spaces in isolated human lung, and AFC was measured. 10^-5 mol/L CGP-12177 combined with phentolamine (α receptor antagonist), atenolol (β₁ receptor antagonist), ICI-118551 (β₂ receptor antagonist), SR59230A (β₃ receptor antagonist), sodium channel blockers or Na⁺/K⁺-ATPase blockers were perfused into the alveolar space of isolated human lung.RESULTS: 10^-5 mol/L and 10^-6 mol/L CGP-12177 increased AFC significantly in isolated human lung,but 10^-4 mol/L and 10^-7 mol/L CGP-12177 didn't increase AFC. The AFC-stimulating effect of CGP-12177 was decreased by SR59230A, amiloride and ouabain.CONCLUSION: CGP-12177, a β₃-adrenergic agonist, can increase AFC in isolated human lung by promoting the transport of Na⁺-K⁺-ATPase.

Key words: isolated human lung, adrenergic agonist, lung injury

中图分类号:

965.2

李乃静, 李伟, 谷秀, 李岩, 何平. β₃肾上腺素能受体激动剂对离体人肺脏肺泡液体清除的作用[J]. 中国临床药理学与治疗学, 2014, 19(3): 271-274.

LI Nai-jing, LI Wei, GU Xiu, LI Yan, HE Ping. Effect of CGP-12177 on alveolar fluid clearance in isolated human lung[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(3): 271-274.

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β₃肾上腺素能受体激动剂对离体人肺脏肺泡液体清除的作用

Effect of CGP-12177 on alveolar fluid clearance in isolated human lung

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