三叶青黄酮对荷Lewis肺癌鼠免疫抑制相关细胞因子的干预作用

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (3): 275-279.

三叶青黄酮对荷Lewis肺癌鼠免疫抑制相关细胞因子的干预作用

冯正权^1,2, 林晓阳³, 郝皖蓉³

¹浙江省立同德医院肿瘤科,杭州 310012,浙江;
²中国中医科学院广安门医院,北京 100053;
³浙江中医药大学,杭州 310053,浙江

收稿日期:2014-03-14 修回日期:2014-03-14 出版日期:2014-03-26 发布日期:2014-04-10
作者简介:冯正权,男,博士,硕导,副主任医师,研究方向为中医药防治肿瘤的物质基础和临床疗效评价。E-mail: fzhq213@aliyun.com
基金资助:
浙江省自然科学基金(LY12H27012)

Effect of tetrastigma hemsleyanum diels et gilg flavone on the immunosuppressive associated cytokines in Lewis lung cancer mice

FENG Zheng-quan^1,2, LIN Xiao-yang³, HAO Wan-rong³

¹Department of Oncology, Tongde Hospital of Zhejiang Province, Hangzhou 310012, Zhejiang, China;
²China Academy of Chinese Medical Sciences of Guang An Men Hospital, Beijing 100053, China;
³Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China

Received:2014-03-14 Revised:2014-03-14 Online:2014-03-26 Published:2014-04-10

摘要/Abstract

摘要： 目的: 研究三叶青黄酮对荷Lewis肺癌模型小鼠的瘤体及免疫抑制相关细胞因子表达的影响,初步探讨三叶青总黄酮逆转肿瘤免疫逃逸的可能机制。方法: 匀浆接种法建立C57BL/6小鼠Lewis细胞肺癌模型,连续给药 14 d 后观察正常组、模型对照组、三叶青黄酮高、中、低剂量组瘤体增长情况及血清中转化生长因子β₁(TGF-β₁)、前列腺素E₂(PGE₂)、环氧化酶-2(COX-2)表达含量。结果: 瘤体:高剂量组、中剂量组与模型对照组相比较瘤体大小有统计学差异(P<0.05),瘤体大小依次为:模型对照组>低剂量组>中剂量组>高剂量组。血清TGF-β₁表达、PGE₂和COX-2含量:各实验组均明显高于正常组(P<0.01),高剂量组<低剂量组(P<0.05)。结论: 三叶青黄酮高剂量组抑瘤效果较好,中剂量抑瘤效果次之,低剂量组抑瘤效果不明显。三叶青黄酮的抑瘤作用可能与降低外周血中TGF-β₁的表达水平有关,可能通过影响PGE₂、COX-2表达水平,从而达到逆转肿瘤逃逸,起到抗肿瘤作用。

关键词: 三叶青黄酮, 转化生长因子β₁, 免疫逃逸, 环氧化酶-2, 前列腺素E₂

Abstract: AIM: Research on the tetrastigma hemsleyanum diels et gilg flavone (sanyeqing),which influences the tumor volume and the expression level of TGFβ₁, PGE₂,COX-2 of LLC tumor bearing C57BL/6 mice, to explore the possible mechanism of reverse tumor immune escape function of radix tetrastigme to tumor-bearing mice.METHODS: LLC tumor bearing C57BL/6 mice models were established by tumor homogenate inoculation method and were randomly divided into normal group, model control group, high dose group of the total flavonoids from radix tetrastigmae, middle dose group of the total flavones from radix tetrastigmae, low dose group of the total flavonoids from radix tetrastigmae. Stripped the tumor tissue and weighed after 14 days continuous administration. The ELISA method was used to detected the TGFβ₁, PGE₂ and COX-2 content in serum.RESULTS: Tumor volume: compared with the model control group, high-dose group, middle-dose group had significant difference in tumor volume (P<0.05). Tumors size in the order: model control group>low-dose group>middle-dose group> high-dose group. TGF-β₁, PGE₂ and COX-2 content in serum: after the experiment, the experimental groups were significantly higher than the normal group (P<0.01), within groups, high-dose group< low-dose group (P<0.05).CONCLUSION: The tetrastigma hemsleyanum diels et gilg flavone high-dose group had a better effect in suppressing tumor growth, the middle dose group didn't play an effective role, And the low-dose group had the most weak effect in suppressing tumor growth. The tumor suppression effect of tetrastigma hemsleyanum diels et gilg flavone may be related to the lower levels of TGF-β₁ expression in peripheral blood, and may decrease the level of PGE₂, COX-2 expression, so as to achieve reverse tumor escape, to achieve the anti-tumor effect.

Key words: tetrastigma hemsleyanum diels et gilg flavone, TGF-β₁, immune escape, COX-2, PGE₂

中图分类号:

R965.2

冯正权, 林晓阳, 郝皖蓉. 三叶青黄酮对荷Lewis肺癌鼠免疫抑制相关细胞因子的干预作用[J]. 中国临床药理学与治疗学, 2014, 19(3): 275-279.

FENG Zheng-quan, LIN Xiao-yang, HAO Wan-rong. Effect of tetrastigma hemsleyanum diels et gilg flavone on the immunosuppressive associated cytokines in Lewis lung cancer mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(3): 275-279.

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