糖皮质激素联合环磷酰胺对人脐静脉内皮细胞增殖及其细胞间黏附分子-1和单核细胞趋化因子-1表达水平的影响

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (4): 366-370.

糖皮质激素联合环磷酰胺对人脐静脉内皮细胞增殖及其细胞间黏附分子-1和单核细胞趋化因子-1表达水平的影响

李志¹, 张梦莹², 徐亮¹, 陆进明¹, 陈兰芳¹, 毛桐俊¹, 宣丹¹

¹皖南医学院弋矶山医院风湿免疫科,
²皖南医学院弋矶山医院中心实验室,芜湖 241000,安徽

收稿日期:2014-01-15 修回日期:2014-04-22 出版日期:2014-04-26 发布日期:2020-07-24
作者简介:李志,男,硕士,讲师,主治医师,研究方向:结缔组织疾病的发病机制。Tel:0553-5739215 E-mail:zhili-8848@qq.com

Effects of glucocorticoid and cyclophosphamide on the human umbilical vein endothelial cell proliferation and ICAM-1 and MCP-1 expression level

LI Zhi¹, ZHANG Meng-ying², LU Jin-ming¹, XU Liang¹, CHEN Lan-fang¹, MAO Tong-jun¹, XUAN Dan¹

¹Department of Rheumatology,
²Central Laboratory, Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui, China

Received:2014-01-15 Revised:2014-04-22 Online:2014-04-26 Published:2020-07-24

摘要/Abstract

摘要： 目的: 探讨地塞米松、环磷酰胺对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)增殖及其细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)和单核细胞趋化因子-1(monocyte chemoattractant protein-1,MCP-1)基因表达的影响。方法: 对培养的人脐静脉内皮细胞,在不同时间以不同药物浓度分别加入地塞米松和环磷酰胺进行干预。以MTT法检测药物对脐静脉内皮细胞增殖的影响。计算两种药物的半数抑制浓度(IC₅₀)。以地塞米松和环磷酰胺半数抑制浓度分别及联合作用人脐静脉内皮细胞 48 h,以RT-PCR的方法比较3组间人脐静脉内皮细胞ICAM-1和MCP-1的mRNA表达差异。结果: 地塞米松、环磷酰胺均呈时间、剂量依赖性地抑制HUVECs的增殖,地塞米松和环磷酰胺IC₅₀分别为 0.74、1.87 mg/mL。地塞米松与环磷酰胺分别及联合孵育下,人脐静脉内皮细胞ICAM-1和MCP-1 mRNA表达水平较对照组显著下降(P<0.05),且联合作用组显著低于单个药物组(P<0.05)。结论: 糖皮质激素和环磷酰胺抑制血管内皮细胞增生,并抑制血管内皮细胞ICAM-1和MCP-1 mRNA表达,从而对血管起到保护作用并可能为临床治疗血管炎提供理论支持。

关键词: 人脐静脉内皮细胞, 糖皮质激素, 环磷酰胺, 细胞间黏附分子, 单核细胞趋化因子

Abstract: AIM: To investigate the effects of combined use of dexamethasone and cyclophosphamide on the proliferation of human umbilical vein endothelialcells (HUVECs) and the expression of mRNA of intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1). METHODS: The HUVECs were respectively incubated for 24,48 or 72 h with dexamethasone or cyclophosphamide with different concentration. The proliferation of HUVECs was determined with methyl thiazolyl tetrazolium(MTT) in different time. The half maximal inhibitory concentration(IC₅₀) was calculated. The levels of mRNA of ICAM-1 and MCP-1 were measured after HUVECs being incubated with dexamethasone and cyclophosphamide respectively and collectively in the concentration of IC50 for 48 hours. RESULTS: Dexamethasone or cyclophosphamide inhibited the proliferation of HUVECs in a dose-and time-dependent manner. The IC₅₀ of dexamethasone and cyclophosphamide was 0.74 mg/mL and 1.87 mg, respectively. The levels of ICAM-1 and MCP-1 mRNA in HUVECs had no difference when HUVECs were incubated with either dexamethasone or cyclophosphamide, yet the levels were significantly decreased as compared with controls (P<0.05). ICAM-1 and MCP-1 mRNA levels in HUVECs were lower as HUVECs being collectively treated with dexamethasone and cyclophosphamide(P<0.05). CONCLUSION: Dexamethasone and cyclophosphamide can significantly inhibit the proliferation of vascular endothelial cells and expression of ICAM-1 and MCP-1, thus producing notable protective effect on the blood vessels. This findings may supply basis for clinical therapy strategy for vasculitis.

Key words: human umbilical vein endothelial cells, glucocorticoid, cyclophosphamide, intercellular adhesion molecules, monocyte chemotactic factor

中图分类号:

R965.2

李志, 张梦莹, 徐亮, 陆进明, 陈兰芳, 毛桐俊, 宣丹. 糖皮质激素联合环磷酰胺对人脐静脉内皮细胞增殖及其细胞间黏附分子-1和单核细胞趋化因子-1表达水平的影响[J]. 中国临床药理学与治疗学, 2014, 19(4): 366-370.

LI Zhi, ZHANG Meng-ying, LU Jin-ming, XU Liang, CHEN Lan-fang, MAO Tong-jun, XUAN Dan. Effects of glucocorticoid and cyclophosphamide on the human umbilical vein endothelial cell proliferation and ICAM-1 and MCP-1 expression level[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(4): 366-370.

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