阿仑膦酸钠对人软骨肉瘤细胞的辅助治疗研究

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (4): 410-414.

阿仑膦酸钠对人软骨肉瘤细胞的辅助治疗研究

乐芳华¹, 楼兴法¹, 方钦华²

¹杭州市萧山区中医院药剂科,杭州 311201,浙江;
²杭州市萧山区中医院骨科,杭州 311201,浙江

收稿日期:2013-07-30 修回日期:2014-03-11 出版日期:2014-04-26 发布日期:2020-07-24
作者简介:乐芳华,女,本科,副主任药师,主要从事临床药学、医院药学研究。Tel:18268136261 E-mail:lefhhzh@163.com

Study of adjuvant treatment of alendronate sodium in human chondrosarcoma cells

LE Fang-hua¹, LOU Xing-fa¹, FANG Qin-hua²

¹Department of Pharmacy, Hangzhou Xiaoshan District Hospital of TCM, Hangzhou 311201, Zhejiang, China;
²Department of Orthopaedics, Hangzhou Xiaoshan District Hospital of TCM, Hangzhou 311201, Zhejiang, China

Received:2013-07-30 Revised:2014-03-11 Online:2014-04-26 Published:2020-07-24

摘要/Abstract

摘要： 目的: 观察阿仑膦酸钠(福善美)在抑制人软骨肉瘤细胞侵袭和迁移中的效果。方法: 软骨肉瘤细胞使用浓度为 100 μmol/L 的阿仑膦酸钠处理,应用明胶酶谱法和基质侵袭实验分别考察阿仑膦酸钠对基质金属蛋白酶活性和瘤细胞迁移及侵袭能力的影响。结果: 阿仑膦酸钠对软骨肉瘤细胞迁移和侵袭的抑制作用呈剂量和时间的依赖性。但本身对肿瘤细胞并没有直接的毒性,明胶酶谱分析和RT-PCR结果还显示,阿仑膦酸钠可以显著降低基质金属蛋白酶的mRNA水平和酶的活性并呈现出浓度依赖的特点。结论: 阿仑膦酸钠可以降低基质金属蛋白酶在转录水平和翻译水平,并抑制软骨肉瘤细胞的侵袭。因此,阿仑膦酸钠可作为治疗全身性软骨肉瘤以及其他恶性肿瘤的辅助治疗药物。

关键词: 阿仑膦酸钠, 双膦酸盐, 软骨肉瘤

Abstract: AIM: To observe the effect of alendronate sodium (fosamax) on inhibition of invasion and migration of human chondrosarcoma cells. METHODS: Human chondrosarcoma cells were treated with 100 μmol/L of alendronate sodium. Matrix metalloproteinase (MMP-2) activity and invasion ability were observed by gelatin zymography and stromal invasion experiment, respectively. RESULTS: The data showed, the degree of inhibition in migration and invasion of chondrosarcoma cells closely was related to the alendronate sodium treated dose and time. In addition, detected with gelatin zymography and RT-PCR, alendronate sodium significantly reduced mRNA levels and enzyme matrix metalloproteinase activity in a concentration related manner. CONCLUSION: Alendronate sodium can reduce the matrix metallo- proteinases in transcription and translation level, and inhibit the invasion of chondrosarcoma cells. Therefore, alendronate sodium, an adjuvant therapy agent, can be used as a systemic treatment for chondrosarcoma and other malignant tumour.

Key words: alendronate sodium, bisphosphonate, chondrosarcoma

中图分类号:

R965.2

乐芳华, 楼兴法, 方钦华. 阿仑膦酸钠对人软骨肉瘤细胞的辅助治疗研究[J]. 中国临床药理学与治疗学, 2014, 19(4): 410-414.

LE Fang-hua, LOU Xing-fa, FANG Qin-hua. Study of adjuvant treatment of alendronate sodium in human chondrosarcoma cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(4): 410-414.

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