新生期芬太尼镇痛下调幼鼠脊髓Fos蛋白表达

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (8): 856-860.

新生期芬太尼镇痛下调幼鼠脊髓Fos蛋白表达

林希, 黄意坚, 吴斌, 张晓燕

福建医科大学附属第一医院儿科,福州 350004,福建

收稿日期:2013-05-06 修回日期:2014-04-09 出版日期:2014-08-26 发布日期:2014-08-26
作者简介:林希,女,硕士,副主任医师,研究方向:小儿神经系统疾病。 Tel: 0591-87981669 E-mail: linxicc@126.com
基金资助:
福建省卫生厅青年科研课题(2011-2-11)

Neonatal Fentanyl analgesia decrease spinal Fos expression in young rats

LIN Xi, HUANG Yi-jian, WU Bin, ZHANG Xiao-yan

Department of Pediatrics, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Fujian ,China

Received:2013-05-06 Revised:2014-04-09 Online:2014-08-26 Published:2014-08-26

摘要/Abstract

摘要： 目的通过观察幼鼠腰骶段脊髓背角Fos蛋白表达,探讨新生期芬太尼镇痛抑制幼鼠慢性内脏痛觉高敏感性形成的作用机制。方法 32只SD新生大鼠按2×2析因设计分成4组,每组8只,A₁B₁组:新生期反复结直肠刺激(colorectal irritation,CI),建立幼鼠内脏痛觉高敏感模型,CI前腹腔注射芬太尼;A₁B₂组:新生期反复CI, CI前腹腔注射生理盐水;A₂B₁组:新生期未接受CI,腹腔注射芬太尼;A₂B₂组:新生期未接受CI,腹腔注射生理盐水。常规饲养到幼鼠期(6周龄),留取L₆-S₂脊髓,应用免疫组织化学染色及计算机图像分析系统进行脊髓Fos阳性细胞数(FLI细胞数)和积分光密度值半定量分析。结果新生期CI前腹腔注射芬太尼镇痛,可使幼鼠腰骶段脊髓背角FLI细胞数和FLI细胞积分光密度值显著减低;幼鼠腰骶段脊髓背角Fos蛋白表达受到新生期CI和新生期应用芬太尼两因素的影响,两者存在交互作用,差别具有统计学意义。结论新生期CI前腹腔注射芬太尼,能够导致幼鼠脊髓神经元Fos蛋白高表达下降,抑制幼鼠慢性内脏痛觉高敏感形成。

关键词: 新生期, 芬太尼镇痛, Fos蛋白, 幼鼠

Abstract: AIM: To investigate the mechanism of blocking visceral hyperalgesia by Fentanyl analgesia in neonatal period through observeing the Fos expression of lumbosacral spinal dorsal horn in visceral hyperalgesia rats. METHODS: Colorectal irritation (CI) stimulation in neonatal period was used to establish the model of visceral hypersensitivity in young rats.32 neonatal rats were divided into four groups by 2×2 factorial design with each 8. Group A₁B₁ was imposed on CI at neonatal period and imposed on fentanyl intraperitoneal injection before CI stimulation; Group A₁B₂ was imposed on CI at neonatal period and administration saline before CI; Group A₂B₁ was not imposed on CI at neonatal period and imposed on fentanyl intraperitoneal injection; Group A₂B₂ was not imposed on CI at neonatal period and not imposed on fentanyl intraperitoneal injection. Then, conventionally breeding till the young period (6-week age), rats were sampled the spinal cord from L6 to S2, the semi-quantity analysis of staining density and the cell numbers of Fos-like immunoreactivity(FLI) of spinal cord were made through immunohisrochemical coloration and computer image analyzing system. RESULTS: The number of FLI and integral opticaldensity of FLI significantly decreased in lumbosacral spinal dorsal horn by neonatal Fentanyl analgesia. The spinal Fos expression in young rats were affected both by CI at neonatal period and neonatal fentanyl analgesia. Futhermore, there were interaction effects between the two factors. CONCLUSION: Neonatal fentanyl analgesia before CI can decrease spinal Fos expression and visceral hypersensitivity in young rats.

Key words: neonatal, fentanyl analgesia, Fos, young rats

中图分类号:

R965.2

林希, 黄意坚, 吴斌, 张晓燕. 新生期芬太尼镇痛下调幼鼠脊髓Fos蛋白表达[J]. 中国临床药理学与治疗学, 2014, 19(8): 856-860.

LIN Xi, HUANG Yi-jian, WU Bin, ZHANG Xiao-yan. Neonatal Fentanyl analgesia decrease spinal Fos expression in young rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(8): 856-860.

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