金丝桃苷体外抗胃癌作用及其机制研究

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (9): 979-983.

金丝桃苷体外抗胃癌作用及其机制研究

孙时华¹, 姜荣华¹, 祝海燕², 姜建军³

¹ 浙江省江山市中医院,江山 324100,浙江;
² 浙江省衢州市人民医院, 衢州 324000,浙江;
³ 浙江省江山市人民医院,江山 324100,浙江

收稿日期:2014-03-07 修回日期:2014-08-04 出版日期:2014-09-26 发布日期:2014-09-26
作者简介:孙时华,女,本科,副主任药师,研究方向:医院药学。 Tel: 15372706655 E-mail: kidneyliu@163.com

Anticancer effects of hyperoside on human gastric cancer cells and its mechanisms

SUN Shi-hua¹, JIANG Rong-hua¹, ZHU Hai-yan², JIANG Jian-jun³

¹ Traditional Chinese Medical Hospital of Jiangshan, Jiangshan 324100, Zhejiang, China;
² Quzhou People's Hospital, Quzhou 324000, Zhejiang, China;
³ People's Hospital of Jiangshan, Jiangshan 324100, Zhejiang, China

Received:2014-03-07 Revised:2014-08-04 Online:2014-09-26 Published:2014-09-26

摘要/Abstract

摘要： 目的研究金丝桃苷(Hyperoside)对胃癌BGC-823细胞的生长、周期及凋亡的作用,并通过检测半胱氨酸天冬氨酸蛋白酶(caspase 3、caspase 8、caspase 9)的活性探讨金丝桃苷诱导胃癌凋亡的可能机制。方法采用MTT法检测金丝桃苷对胃癌BGC-823细胞的增殖影响;流式细胞技术检测金丝桃苷对胃癌BGC-823细胞周期和凋亡的影响;比色法测定金丝桃苷对胃癌BGC-823细胞凋亡过程中caspase 3、caspase 8和caspase 9活性影响。结果金丝桃苷处理BGC-823细胞 24 h 和 48 h 后,细胞生长明显抑制,作用24 h和48 h时其IC₅₀分别为 32.14 和 22.67 μg/mL;金丝桃苷处理组与对照组相比,胃癌BGC-823细胞G₀/G₁期比例明显增加,凋亡细胞比例明显增加;caspase 3、caspase 8和caspase 9活性随药物浓度增加而逐渐升高。结论金丝桃苷能够通过阻断细胞周期、诱导细胞凋亡有效抗胃癌,其诱导肿瘤细胞凋亡机制可能与激活caspase 3、caspase 8和caspase 9活性密切相关。

关键词: 金丝桃苷, 胃癌BGC-823细胞, 细胞周期, 细胞凋亡

Abstract: AIM: To observe the effects of hyperoside on the cell proliferation, cycle and apoptosis of human gastric cancer cell line BGC-823 and to explore its possible mechanisms. METHODS: The effects of hyperoside on the proliferation of BGC-823 cells were evaluated by MTT assay. Flow cytometry was used to analyze the cell cycle and apoptosis of the cells exposed to hyperoside. The activities of caspase 3, caspase 8 and caspase 9 were examined by spectrophotometry. RESULTS: With the hyperoside incubation for 24 h or 48 h resulted in a significant inhibition of proliferation in BGC-823 cells as a IC₅₀ of 32.14 μg/mL and 22.67 μg/mL. After treatment with hyperoside,the cell cycle was suppressed obviously at G₀/G₁ phase. The apoptotic rates were significantly higher in the cells treated with hyperoside than in the control. Comparing with the control, the activities of caspase 3, caspase 8 and caspase 9 were up-regulated in hyperoside group. CONCLUSION: Hyperoside have significant anti-gastric tumor effects by induce cell cycle arrest and apoptosis of BGC-823 cells, and intracelular caspase 3, caspase 8 and caspase 9 are closely associated with the apoptosis.

Key words: hyperoside, BGC-823 cells, cell cycle, apoptosis

中图分类号:

R965.2

孙时华, 姜荣华, 祝海燕, 姜建军. 金丝桃苷体外抗胃癌作用及其机制研究[J]. 中国临床药理学与治疗学, 2014, 19(9): 979-983.

SUN Shi-hua, JIANG Rong-hua, ZHU Hai-yan, JIANG Jian-jun. Anticancer effects of hyperoside on human gastric cancer cells and its mechanisms[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 979-983.

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