免疫性非细菌性前列腺炎大鼠内环境因素关联性研究

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (9): 995-1000.

免疫性非细菌性前列腺炎大鼠内环境因素关联性研究

周莉¹, 王永¹, 骆永伟^{1, 2}, 王蓉¹, 孟祥^{1, 2}, 贾玉玲^{1, 2}, 刘向云¹, 孙祖越¹

¹ 上海市计划生育科学研究所药理毒理学研究室,中国生育调节药物毒理检测中心,上海 200032;
² 复旦大学药学院,上海 201203

收稿日期:2014-01-01 修回日期:2014-06-30 出版日期:2014-09-26 发布日期:2014-09-26
通讯作者: 孙祖越,男,博士后,研究员,主要从事药物生殖药理毒理学研究、前列腺疾病药理毒理学研究和新药临床前安全性评价。 Tel: 021-64043044 E-mail: sunzy64@163.com
作者简介:周莉,女,博士后,副研究员,主要从事药物生殖药理毒理学研究、前列腺疾病药理毒理学研究和新药临床前安全性评价。 Tel: 13585702671 E-mail: zhoulijss@163.com
基金资助:
十二五“重大新药创制”科技重大专项(2011ZX09301-005); 上海市人才发展基金(2010031); 上海市实验动物创新行动计划项目(11140901300/11140901303)

Correlation effect change of internal environmental in prostate antigen protein induced SD rat prostatitis

ZHOU Li¹, WANG Yong¹, LUO Yong-wei^{1, 2}, WANG Rong¹, MENG Xiang^{1, 2}, JIA Yu-ling^{1, 2}, LIU Xiang-yun¹, SUN Zu-yue¹

¹ Shanghai Institute of Planned Parenthood Research & National Evaluation Centre for Toxicology of Fertility Regulating Drugs, Shanghai 200032, China;
² School of Pharmacy, Fudan University, Shanghai 201203, China

Received:2014-01-01 Revised:2014-06-30 Online:2014-09-26 Published:2014-09-26

摘要/Abstract

摘要： 目的探讨前列腺组织蛋白结合免疫佐剂法诱导的SD大鼠非细菌性前列腺炎的炎症反应与内环境因素之间的关系。方法 30只大鼠随机分成3组,其中一组为阴性对照组,其它两组为试验组,第0、7天多点皮内注射浓度分别为20、40 mg/mL 的大鼠前列腺蛋白提纯液联合完全弗氏佐剂(1∶1的混悬液)1.0 mL,腹腔注射百白破疫苗 0.5 mL,第21天皮内注射大鼠前列腺蛋白提纯液 0.5 mL,阴性对照组在相同时间和部位注射同等剂量的生理盐水,造模后第55天处死大鼠。结果前列腺蛋白提纯液联合完全弗氏佐剂制作诱导SD大鼠前列腺炎后,可见前列腺间质有大量炎细胞浸润,以淋巴细胞为主,呈现明显的慢性炎症表现。免疫因素引起的慢性非细菌性前列腺炎可导致免疫球蛋白G(IgG)浓度明显升高(P<0.05),免疫球蛋白M(IgM)浓度明显降低(P<0.05),C-反应蛋白(CRP)和前列腺特异抗原(PSA)含量明显增加(P<0.05)。结论根据本实验的结果,免疫性非细菌性前列腺炎大鼠体内IgG、IgM、CRP和PSA含量呈现关联性变化。

关键词: 免疫性前列腺炎, 慢性非细菌性前列腺炎, SD大鼠

Abstract: AIM: To explore the internal environment correlation prostate antigen protein induced SD male rats with chronic prostatitis. METHODS: 30 male rats were randomly divided into 3 groups, each one contained 10 ones. One was negative control group; the other two groups were the experimental group. 20, 40 mg/mL of rat prostate protein purification liquid combined with complete freund's adjuvant (1∶1 mixed suspension) were injected respectively in SD rats by multipoint intradermal injection at 0, 7 days and 0.5 mL of DPT vaccine was injected by intraperitoneal injection. At 21 days, 0.5 mL 20, 40 mg/mL of rat prostate protein purification liquid combined with complete freund's adjuvant were injected respectively in SD rats by intradermal injection. The negative control group was injected by equal dose of normal saline at the same time and place. RESULTS: Histopathological analyses indicated that there was inflammatory infiltrates in the experimental groups prostate stromal, which showed obvious chronic inflammation. Immune factors of chronic nonbacterial prostatitis can lead to IgG concentration increased significantly (P<0.05), CRP and PSA levels increased significantly (P<0.05). CONCLUSION: According to the experimental results, chronic non-bacterial prostatitis induced by prostate antigen protein lead to IgG concentration, CRP and PSA levels increased significantly.

Key words: autoimmunity prostatitis, chronic nonbacterial prostatitis, SD rat

中图分类号:

R965.1

周莉, 王永, 骆永伟, 王蓉, 孟祥, 贾玉玲, 刘向云, 孙祖越. 免疫性非细菌性前列腺炎大鼠内环境因素关联性研究[J]. 中国临床药理学与治疗学, 2014, 19(9): 995-1000.

ZHOU Li, WANG Yong, LUO Yong-wei, WANG Rong, MENG Xiang, JIA Yu-ling, LIU Xiang-yun, SUN Zu-yue. Correlation effect change of internal environmental in prostate antigen protein induced SD rat prostatitis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 995-1000.

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