经典Wnt信号途径相关因子与大肠癌分期的关系

摘要/Abstract

摘要： 目的: 探讨经典Wnt信号途径相关因子与大肠癌分期的关系。方法: 搜集2013年1月1日至2014年1月1日皖南医学院第一附属医院胃肠外科收治的46例大肠癌患者的临床资料、血清样本及病理组织标本,根据Dukes分期分为四组。搜集10例正常的肠黏膜组织标本作为对照组1,应用免疫组化法检测大肠癌及正常肠黏膜组织中Wnt-2蛋白、β-catenin蛋白、Axin蛋白、APC蛋白的表达。搜集15例健康人的血清样本作为对照组2,应用酶联免疫吸附试验检测大肠癌患者和对照组2血清中Wnt-2蛋白、Axin蛋白的表达。结果: Wnt-2、β-catenin在大肠癌组织中高表达,Axin、APC在大肠癌组织中低表达,且各个分期之间差异有统计学意义;46例大肠癌患者血清中Wnt-2浓度明显高于健康人,各个分期之间的差异也有统计学意义。Axin血清浓度低于健康人,但是各个分期之间的差异无统计学意义。结论: 经典Wnt信号途径相关蛋白的表达与大肠癌Dukes分期有关,经典Wnt信号途径相关因子可作为大肠癌筛查的血清学辅助指标,有助于提高大肠癌检出的灵敏度。

关键词: 经典Wnt信号途径, 大肠癌, Wnt-2蛋白, β-catenin蛋白, Axin蛋白, APC蛋白

Abstract: AIM: To evaluate the relationship between the related factors of Wnt/β-catenin signaling pathways and the stages of colorectal cancer.METHODS: We collected the clinical data, serum specimens and pathological tissue samples of 46 cases of patients with colorectal cancer who admitted in gastrointestinal surgery of the first affiliated hospital of Wannan Medical college from January 1, 2013 to January 1, 2014. According to the Dukes staging, these was divided into four groups. 10 cases of normal intestinal mucosa specimens were recruited as control group 1. The protein expression of Wnt-2, β-catenin, Axin, APC were detected by using immunohistochemical methods. 15 serum samples of healthy individuals were recruited as control group 2. The concentration of Wnt-2, β-catenin, Axin, APC in serum were detected by using enzyme-linked immunosorbent assay.RESULTS: The protein expression of Wnt-2 and β-catenin in colorectal cancer tissue were significantly higher than that in the normal intestinal mucosa, while the Axin and APC were lower than that in the normal intestinal mucosa, and the differences between different colorectal cancer stagings were statistically significant. The concentration of Wnt-2 in 46 cases of colorectal cancer patients serum was significantly higher than that in healthy people, and the differences between different colorectal cancer staging have statistical significances. The serum concentration of Axin was lower than in the healthy people, but there was no statistical difference between different colorectal cancer stagings.CONCLUSION: The expression of the related factors of Wnt/β-catenin signaling pathways was associated with colorectal cancer Dukes staging. The related factors of Wnt/β-catenin signaling pathways can be used as auxiliary serum examination index, contributing to improve colorectal cancer detection sensitivity.

Key words: canonical Wnt pathways, colorectal cancer, Wnt-2, β-catenin, Axin, APC

中图分类号:

陶坤, 赵国海, 张义胜, 徐华平, 陈方正, 张晓峰, 吴泽晖. 经典Wnt信号途径相关因子与大肠癌分期的关系[J]. 中国临床药理学与治疗学, 2015, 20(4): 414-419.

TAO Kun, ZHAO Guo-hai, ZHANG Yi-sheng, XU Hua-ping, CHEN Fang-zheng, ZHANG Xiao-feng, WU Ze-hui. Relationship between related factors of canonical Wnt pathways and colorectal cancer staging[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(4): 414-419.

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