花参子露对小鼠慢性湿疹模型的疗效研究

中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (12): 1358-1363.

花参子露对小鼠慢性湿疹模型的疗效研究

严丹丹¹，黄芳¹，徐丽华²

¹中国药科大学，南京 211100，江苏；² 苏州思源天然产物研发有限公司，苏州 215000，江苏

收稿日期:2017-04-06 修回日期:2017-05-04 出版日期:2017-12-26 发布日期:2018-01-02
通讯作者: 黄芳，女，博导，教授，研究方向：中药药理学。 Tel：18151620528 E-mail:chengtianle007@163.com
作者简介:严丹丹，女，硕士研究生，研究方向：中药药理学。 Tel：15951077939 E-mail:592418550@qq.com

Effects of Huashenzilu on chronic eczema in mice model

YAN Dandan¹, HUANG Fang ¹, XU Lihua²

¹Chineses Pharmaceutical University, Nanjing 211100, Jiangsu, China;² Sylvan Natural Product R&D, Suzhou 215000, Jiangsu, China

Received:2017-04-06 Revised:2017-05-04 Online:2017-12-26 Published:2018-01-02

摘要/Abstract

摘要：

目的: 研究花参子露对小鼠慢性湿疹模型的治疗作用。方法: 采用2，4-二硝基氯苯（DNCB）反复激发小鼠耳部造成小鼠湿疹模型，给予不同剂量花参子露治疗之后，ELISA法检测小鼠血清中IL-2、IL-4、TNF-α、IFN-γ含量，HE染色观察小鼠组织病理学改变，以及按照评分标准对小鼠耳部进行评分，计算小鼠耳部肿胀度以及小鼠搔抓指数。结果: 花参子露治疗后，小鼠血清中IL-2、IFN-γ水平下降，IL-4、TNF-α水平上升，耳部肿胀程度，耳部评分以及搔抓指数明显下降，与模型组相比有统计学差异（P<0.01）；HE染色结果显示，不同剂量药物治疗后小鼠耳部皮肤表皮增厚，棘皮层空泡化，真皮层嗜酸性粒细胞、肥大细胞、巨噬细胞等浸润现象均得到不同程度的改善。结论: 花参子露对小鼠慢性湿疹模型具有一定治疗作用，其可能是通过调节Th1型以及Th2型免疫反应的平衡来达到治疗作用。

关键词: 花参子露, 慢性湿疹, 免疫反应

Abstract:

AIM: To observe clinical the effects of Huashenzilu on mice models with acute and chronic eczema. METHODS: Eczema was induced by repeated local exposure to DNCB, and then the mice received Huashenzilu. Using ELISA kit method to test the serum concentration of IL-2, IL-4，TNF-alfa，IFN-gamma, and HE staining to test histopathological changes. The ear swelling and scratching index were measured, and ear allergy score were measured according to the standard for evaluation. RESULTS: After the treatment of Huashenzilu, the serum concentration of IL-4，TNF-alfa significantly increased and the serum concentration of IL-2，IFN-gamma significantly decreased in the chronic mice compared to the model group (P<0.01). The ear swelling, scratching index and ear allergy score were significantly decreased. The phenomenon of epidermal thickening, acanthosis layer cavitation, and mast cells, macrophages infiltration were improved compared to the model group. CONCLUSION: Huashenzilu can control the chronic eczema in mice through the mechanism of regulating the balance between Th1 and Th2 immune response.

Key words: Huashenzilu, chronic eczema, immune response

中图分类号:

R965.2

严丹丹，黄芳，徐丽华. 花参子露对小鼠慢性湿疹模型的疗效研究[J]. 中国临床药理学与治疗学, 2017, 22(12): 1358-1363.

YAN Dandan, HUANG Fang, XU Lihua. Effects of Huashenzilu on chronic eczema in mice model[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(12): 1358-1363.