基于肿瘤相关巨噬细胞的宫颈癌免疫学病理机制研究

中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (12): 1394-1399.

基于肿瘤相关巨噬细胞的宫颈癌免疫学病理机制研究

陈星¹，吴为玲²，陈姗姗¹，叶丽娅¹，梅双双¹，王静¹，何钰¹，柴泽英¹

¹温州医科大学附属浙江省台州医院妇产科，台州 317000，浙江；²温州医科大学附属恩泽医疗中心妇产科医院妇产科，台州 317000，浙江

收稿日期:2017-06-29 修回日期:2017-08-11 出版日期:2017-12-26 发布日期:2018-01-02
作者简介:陈星，女，博士，主任医师，研究方向：宫颈癌的基础和临床研究。 Tel：13757604765 E-mail：faa0323@163.com
基金资助:
台州市科学技术局项目（1601KY75-12）

Immunological pathology mechanism research of cervical cancer based on tumor related macrophage

CHEN Xing¹, WU Weiling², CHEN Shanshan¹, YE Liya¹, MEI Shuangshuang¹, WANG Jing¹, HE Yu¹, CHAI Zeying¹

¹ Department of Gynaecology and Obstetrics, Taizhou Hospital Affiliated to Wenzhou Medical University, Taizhou 317000, Zhejiang, China; ² Department of Gynaecology and Obstetrics, Maternity Hospital of Enze Medical Centre Affiliated to Wenzhou Medical University, Taizhou 317000, Zhejiang, China

Received:2017-06-29 Revised:2017-08-11 Online:2017-12-26 Published:2018-01-02

摘要/Abstract

摘要：

目的:分析肿瘤相关巨噬细胞（TAMs）在宫颈癌发病中的作用机制，明确其与Th1/Th2比值、Th17/CD4+CD25+Foxp3+Treg比值的相关性。方法: 以27例宫颈癌患者和53例宫颈上皮内瘤变（CINⅡ22例、CINⅢ31例）患者为研究对象，治疗前抽取患者空腹肘静脉血，采用流式细胞仪测定Th1/Th2、Th17/CD4+CD25+Foxp3+Treg比值，采用ELISA试剂盒测定血清IFN-γ、IL-4、IL-17A、IL-17F、TGF-β1、IL-10水平；术中留取病理组织，采用免疫组化实验测定TAMs CD68表达。结果: 宫颈癌患者外周血Th1/Th2与Th17/CD4+CD25+Foxp3+Treg比值低于CINⅢ，CINⅢ低于CINⅡ，各组之间的差异有统计学意义（P<0.05）。宫颈癌患者血清IL-4、TGF-β1、IL-10水平高于CINⅢ，CINⅢ高于CINⅡ，血清INF-γ、IL-17A、IL-17F水平低于CINⅢ，CINⅢ低于CINⅡ，各组之间的差异有统计学意义（P<0.05）。宫颈癌患者病灶组织TAMs CD68表达水平高于CINⅢ，CINⅢ高于CINⅡ，各组之间差异均有统计学意义（P<0.05）。相关性分析结果显示TAMs CD68表达水平与Th1/Th2比值、Th17/CD4+CD25+Foxp3+Treg比值及IL-17A水平呈负相关性，与IL-10、IL-4水平呈正相关性（P<0.05）。结论:宫颈癌TAMs与Th1/Th2、Th17/CD4+CD25+Foxp3+Treg密切相关，可能通过影响这两大体系的平衡介导宫颈癌的发生与发展。

Abstract:

AIM:To analyze the mechanism of tumor-associated macrophage (TAMs) in the development of cervical cancer and to investigate its correlation with Th1/Th2 and Th17/CD4+CD25+Foxp3+Treg. METHODS:Twenty seven cases of cervical cancer and 53 cases of cervical intraepithelial neoplasias (including 22 cases of CINⅡ and 31 cases of CINⅢ) were selected as subjects. The venous blood of patients before treatment was extracted to detect Th1/Th2 and Th17/CD4+CD25+Foxp3+Treg with flow cytometry, and detect serum IFN-γ, IL-4, IL-17A, IL-17F, TGF-β1 and IL-10 levels with ELISA Kits. Furthermore, pathological tissues were extracted during operation, and its TAMsCD68 expression was detected by immunohistochemistry technique. RESULTS:The Th1/Th2 and Th17/CD4+CD25+Foxp3+Treg of cervical cancer were both lower than those of CINⅢ, and those of CINⅢ were both lower than CINⅡ, the difference between groups had statistical significance (P<0.05). The serum IFN-γ, IL-4, IL-17A, IL-17F, TGF-β1 and IL-10 levels of cervical cancer were all higher than those of CINⅢ, and those of CINⅢ were all higher than CINⅡ, the difference between groups had statistical significance (P<0.05). The TAMsCD68 expression level of cervical cancer was higher than that of CINⅢ, and that of CINⅢ was lower than CINⅡ, the difference between groups had statistical significance (P<0.05). The correlation analysis results showed TAMsCD68 expression level had negative correlations with Th1/Th2, Th17/CD4+CD25+Foxp3+Treg, and serum IL-17A level, whereas presented positive correlations with serum IL-10 and IL-4 level (P<0.05). CONCLUSION:TAMs is closely related with Th1/Th2 and Th17/CD4+CD25+Foxp3+Treg in cervical cancer, and possibly is mediating the occurrence and development of cervical cancer through influencing the balance of these two systems.

Key words: tumor-associated macrophage, cervical cancer, Th1/Th2, Th17/CD4+CD25+Foxp3+Treg

中图分类号:

R737.3

陈星，吴为玲，陈姗姗，叶丽娅，梅双双，王静，何钰，柴泽英. 基于肿瘤相关巨噬细胞的宫颈癌免疫学病理机制研究[J]. 中国临床药理学与治疗学, 2017, 22(12): 1394-1399.

CHEN Xing, WU Weiling, CHEN Shanshan, YE Liya, MEI Shuangshuang, WANG Jing, HE Yu, CHAI Zeying. Immunological pathology mechanism research of cervical cancer based on tumor related macrophage[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(12): 1394-1399.

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