人参皂苷C-K对匹罗卡品致痫大鼠发作的影响

中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (2): 139-143.

人参皂苷C-K对匹罗卡品致痫大鼠发作的影响

曾祥昌¹，周露萍¹，陈露露¹，罗伟¹，李超鹏¹，高青¹，王亚芹¹，王欣桐¹，胡凯²，沈杰³，欧阳冬生¹

¹中南大学湘雅医院临床药理研究所，中南大学临床药理研究所，遗传药理学湖南省重点实验室，长沙 410078，湖南； ²中南大学湘雅医院神经内科，长沙 410008，湖南；3皖南医学院药学院，芜湖 241001，安徽

收稿日期:2016-10-18 修回日期:2016-12-07 出版日期:2017-02-26 发布日期:2017-03-02
通讯作者: 欧阳冬生，男，博士，教授，博士生导师，研究方向：临床药理学及天然药物研究。 Tel：0731-84805380 E-mail：ouyangyj@163.com
作者简介:曾祥昌，男，在读硕士，研究方向：中药与天然药物药理学。 Tel：18274802795 E-mail：zxchang45168@163.com
基金资助:
国家自然科学基金（81301106，81503236，81641141）；湖南省自然科学基金(14JJ4006)；湖南省自然科学基金 (2016JJ4116)；安徽高校自然科学研究重点项目( KJ2015A155)

Effects of ginsenoside C-K on seizure activity in pilocarpine-induced epileptic rats

ZENG Xiangchang ¹, ZHOU Luping ¹, CHEN Lulu¹, LUO Wei ¹, LI Chaopeng¹, GAO Qing¹, WANG Yaqin ¹, WANG Xintong ¹, HU Kai ², SHEN Jie ³, OUYANG Dongsheng ¹

¹ Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, Hunan, China；²Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China; ³ Pharmacy School of Wannan Medical College, Wuhu 241001, Anhui, China

Received:2016-10-18 Revised:2016-12-07 Online:2017-02-26 Published:2017-03-02

摘要/Abstract

摘要：

目的:探讨人参皂苷C-K对癫痫大鼠行为学影响及其与拉莫三嗪的协同作用。方法:取70只雄性SD大鼠随机分为正常对照组、模型组、拉莫三嗪组、人参皂苷C-K低、中、高剂量组、拉莫三嗪与人参皂苷合用组，各组大鼠多次给药后腹腔注射氯化锂-匹罗卡品造模，观察大鼠行为变化，利用Racine分级标准评估癫痫发作强度，记录癫痫发作潜伏期。结果:与癫痫模型组相比，人参皂苷C-K显著延长癫痫发作潜伏期，降低癫痫发作后体质量丢失（n=10,P＜0.05）。人参皂苷C-K与拉莫三嗪合用显著降低癫痫发作强度，进一步延长癫痫发作潜伏期（n=10,P＜0.05）。结论:人参皂苷C-K具有抗癫痫作用，与拉莫三嗪合用具有协同增强抗癫痫作用。

关键词: 人参皂苷C-K, 拉莫三嗪, 癫痫, 发作

Abstract:

AIM: To investigate the effects of ginsenoside C-K on behavior in epileptic rats and its synergistic effect with lamotrigine. METHODS: 70 male SD rats were randomly divided into normal group, model group, lamotrigine group, ginsenoside C-K low, middle, high group, lamotrigine and ginsenoside C-K combination group. Rats in model group and treatment group were prepared for epilepsy animal model by intraperitoneal injection of lithium chloride-pilocarpine after administration of lamotrigine or ginsenoside C-K. Behavior changes were closely observed by video monitoring system after pilocarpine. Behavioural seizure activity was scored according to Racine grading standard and recorded seizure latency. RESULTS: After treatment of ginsenoside C-K, the treatment group rats exhibited significant an increase in seizure latency compared with epilepsy model group. The loss of body wieght reduced significantly in these surviving animals pretreated with ginsenoside C-K(n=10, P＜0.05). Intriguingly, the complex of ginsenoside C-K and lamotrigine reduces seizure intensities, seizure latency was further prolonged(n=10, P＜0.05). CONCLUSION: Ginsenoside C-K can exhibit an antiepileptic effect. The combination of ginsenoside C-K and lamotrigine have synergistic anti-epileptic effect.

Key words: ginsenoside C-K, lamotrigine, epilepsy, seizure activity

中图分类号:

R965.2

曾祥昌，周露萍，陈露露，罗伟，李超鹏，高青，王亚芹，王欣桐，胡凯，沈杰，欧阳冬生. 人参皂苷C-K对匹罗卡品致痫大鼠发作的影响[J]. 中国临床药理学与治疗学, 2017, 22(2): 139-143.

ZENG Xiangchang, ZHOU Luping, CHEN Lulu, LUO Wei, LI Chaopeng, GAO Qing, WANG Yaqin, WANG Xintong, HU Kai, SHEN Jie, OUYANG Dongsheng. Effects of ginsenoside C-K on seizure activity in pilocarpine-induced epileptic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(2): 139-143.

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