柚皮苷通过下调p70S6K抑制非小细胞肺癌细胞生长的作用机制研究

中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (7): 762-766.

柚皮苷通过下调p70S6K抑制非小细胞肺癌细胞生长的作用机制研究

高中原，孙更，李敏，谌茜，李萍，王雪融

南京医科大学基础医学院药理学系，南京 211166，江苏

收稿日期:2017-04-05 修回日期:2017-05-04 出版日期:2017-07-26 发布日期:2017-07-19
通讯作者: 王雪融，女，博士，教授，研究方向：肿瘤药理。 Tel：13813385723 E-mail:wangxr@njmu.edu.com
作者简介:高中原，男，硕士，研究方向：肿瘤药理。 Tel:18251817201 E-mail:gzyyuan@126.com
基金资助:
国家自然科学基金（81473241，81102458，81172004）

Naringin inhibits the growth of non-small cell lung carcinoma cells through down-regulating p70S6K

GAO Zhongyuan, SUN Geng, LI Min, CHEN Xi, LI Ping, WANG Xuerong

Department of Pharmacology, Nanjing Medical University, Nanjing 211166, Jiangsu, China

Received:2017-04-05 Revised:2017-05-04 Online:2017-07-26 Published:2017-07-19

摘要/Abstract

摘要：

目的: 研究柚皮苷对非小细胞肺癌细胞生长的作用及机制。方法: 应用SRB法检测柚皮苷对非小细胞肺癌细胞株生长的抑制作用。用Western blot法检测柚皮苷对p70S6K的调控作用。利用质粒过表达p70S6K，用SRB法检测过表达p70S6K后对柚皮苷抑制细胞生长作用的影响。利用小干扰RNA阻抑p70S6K的表达，用SRB法检测阻抑p70S6K的表达对柚皮苷抑制细胞生长作用的影响。结果: 柚皮苷呈浓度依赖性抑制非小细胞肺癌细胞的生长，并且可以下调p70S6K的表达。过表达p70S6K可以削弱柚皮苷抑制细胞生长的作用，敲降p70S6K可以增强柚皮苷抑制细胞生长的作用。结论: 柚皮苷通过下调p70S6K抑制非小细胞肺癌细胞的生长。

关键词: p70S6K, 柚皮苷, 非小细胞肺癌细胞

Abstract:

AIM: To study the effect of naringin on the growth of non-small cell lung carcinoma cells (NSCLC) and its mechanism. METHODS: SRB method was employed to investigate the inhibitory effect of naringin on the growth of non-small cell lung carcinoma cell lines. Western blot was used to detect the p70S6K expression. The plasmid encoding full length p70S6K was transfected to over-express p70S6K. The small-interfering RNA was used to knockdown p70S6K expression. RESULTS:Naringin inhibited the growth of NSCLC cells, and down-regulated the expression of p70S6K. The over-expression of p70S6K partially abrogated the inhibitory effect of naringin, while knockdown of p70S6K enhanced the effect of naringin. CONCLUSION: Naringin can down-regulate the p70S6K to inhibit the growth of NSCLC cells.

Key words: p70S6K, naringin, non-small cell lung carcinoma cells

中图分类号:

R734.2

高中原，孙更，李敏，谌茜，李萍，王雪融. 柚皮苷通过下调p70S6K抑制非小细胞肺癌细胞生长的作用机制研究[J]. 中国临床药理学与治疗学, 2017, 22(7): 762-766.

GAO Zhongyuan, SUN Geng, LI Min, CHEN Xi, LI Ping, WANG Xuerong. Naringin inhibits the growth of non-small cell lung carcinoma cells through down-regulating p70S6K[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(7): 762-766.

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