依托咪酯对大鼠海马兴奋性突触传递的影响

中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (7): 775-778.

依托咪酯对大鼠海马兴奋性突触传递的影响

魏辉明，刘超，杨云丽

解放军昆明总医院麻醉科，昆明 650032，云南

收稿日期:2016-12-28 修回日期:2017-01-12 出版日期:2017-07-26 发布日期:2017-07-19
通讯作者: 杨云丽，女，硕士，副主任医师，研究方向：临床麻醉药理学。 Tel：0871-4774724 　E-mail：ynyangyunli@qq.com
作者简介:魏辉明，男，博士，主任医师，研究方向：麻醉及疼痛临床药理学。 Tel：0871-4774724 　E-mail：medicana@sina.com
基金资助:
成都军区“十二五”医学科研项目(B14013)

Effect of etomidate on excitatory synaptic transmission efficacy in rat hippocampus

WEI Huiming, LIU Chao, YANG Yunli

Department of Anesthesiology, Kunming General Hospital of PLA, Kunming 650032, Yunnan, China

Received:2016-12-28 Revised:2017-01-12 Online:2017-07-26 Published:2017-07-19

摘要/Abstract

摘要：

目的: 探讨依托咪酯对大鼠海马CA1区兴奋性突触传递的影响及其机制。方法: 成年雄性Wistar大鼠24只，随机分为四组(n=6)：对照组、荷包牡丹碱组、依托咪酯和依托咪酯＋荷包牡丹碱组。配对刺激方波波宽0.1 ms、频率0.033 Hz、刺激间隔30 ms，刺激强度为诱发海马CA1区兴奋性突触后电位(EPSP)峰值强度的50%。在体记录四组EPSP幅值的基础值，随后腹腔分别注射生理盐水3 mL/kg、荷包牡丹碱2 mg/kg、依托咪酯10 mg/kg和等剂量依托咪酯+荷包牡丹碱。各组给药后再记录60 min配对刺激下的两个EPSP幅值（分别为E1和E2），计算各时段E1、E2和E2/E1较基础值的相对幅值。结果: 与对照组比较，依托咪酯组E1相对幅值降低(P<0.05)，荷包牡丹碱组和托咪酯＋荷包牡丹碱组差异无统计学意义(P>0.05)；与对照组比较，荷包牡丹碱组E2及E2/E1升高 (P<0.05)，依托咪酯组降低(P<0.05)；与荷包牡丹碱组比较，依托咪酯组和托咪酯＋荷包牡丹碱组E2及E2/E1降低(P<0.05)。结论: 依托咪酯可抑制大鼠海马CA1区兴奋性突触传递，机制可能与增强突触前GABA能性抑制性回路的兴奋性有关。

关键词: 依托咪酯, 突触传递, 海马

Abstract:

AIM: To investigate the effect of etomidate on excitatory synaptic transmission efficacy in stratum radiatum of CA1 area of rat hippocampus and the underlying mechanism. METHODS: Twenty-four adult male Wistar rats were randomly divided into 4 groups (n=6 each): control group, bicuculline group, etomidate group and etomidate + bicuculline group. The animals were anesthetized with intraperitoneal (IP) pentobarbital 50 mg/kg. The stimulating and recording electrodes were inserted into the stratum radiatum of CA1 area of the hippocampus. The paired pulse stimulation with the interval 30 ms between the 2 pulse stimulation was used. Excitatory post-synaptic potential (EPSP) was elicited in vivo before (baseline) and after IP bicuculline (2 mg/kg) or/and etomidate (10 mg/kg) administration. The EPSP2/EPSP1 (E2/E1) ratio was recorded during paired pulse stimulation.RESULTS:Compared with control group, the E1 amplitude was significantly decreased in etomidate group (P<0.05), but no significant change was found in bicuculline group and etomidate + bicuculline group (P>0.05). Compared with control group, E2 and E2/E1 ratio were significantly increased in bicuculline group, while significantly decreased in etomidate group (P<0.05). E2 and E2/E1 ratio were significantly lower in etomidate group and etomidate + bicuculline group than in bicuculline group (P<0.05). CONCLUSION: The results suggest that etomidate might depress the excitatory synaptic transmission efficacy in the CA1 area of the rat hippocampus through presynaptic GABA energic inhibitory circuit.

Key words: etomidate, synaptic transmission, hippocampus

中图分类号:

R965.2

魏辉明，刘超，杨云丽. 依托咪酯对大鼠海马兴奋性突触传递的影响[J]. 中国临床药理学与治疗学, 2017, 22(7): 775-778.

WEI Huiming, LIU Chao, YANG Yunli . Effect of etomidate on excitatory synaptic transmission efficacy in rat hippocampus[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(7): 775-778.

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