Th17细胞在痤疮发病中的作用及与痤疮严重度相关性的功能研究

doi:10.12092/j.issn.1009-2501.2018.01.016

中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (1): 83-87.doi: 10.12092/j.issn.1009-2501.2018.01.016

Th17细胞在痤疮发病中的作用及与痤疮严重度相关性的功能研究

陆威^1,2，周炳荣³，汤怡^1,2，樊一斌^1,2，陶小华^1,2

¹ 浙江省人民医院皮肤科，杭州 310014，浙江；²杭州医学院附属人民医院皮肤科，杭州 310014，浙江；³ 南京医科大学第一附属医院皮肤科，南京 210029，江苏

收稿日期:2017-08-15 修回日期:2017-10-16 出版日期:2018-01-26 发布日期:2018-02-07
通讯作者: 陶小华，女，硕士，主任医师，研究方向：色素性皮肤病和性传播疾病的诊治。 Tel：13505811700 E-mail:13505811700@163.com
作者简介:陆威，女，硕士，主治医师，研究方向：损容性皮肤病的诊治及机制研究。 Tel：13067701943 E-mail：cak0318@163.com
基金资助:
浙江省医药卫生科技项目（2015KYA020）

Role of Th17 cells in acne pathogenesis and the correlation with severity of acne

LU Wei ^1,2, ZHOU Bingrong ³, TANG Yi ^1,2, FAN Yibin ^1,2, TAO Xiaohua ^1,2

¹ Department of Dermatology, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang, China; ²Department of Dermatology, People's Hospital of Hangzhou Medical College, Hangzhou 310014, Zhejiang, China; ³Department of Dermatology, the First Affiliated Hospital with Nanjing Medical University, Nanjing 210029, Jiangsu, China

Received:2017-08-15 Revised:2017-10-16 Online:2018-01-26 Published:2018-02-07

摘要/Abstract

摘要：

目的: 探究Th17细胞在痤疮发生过程中的作用机理及与痤疮严重度的相关性。方法: 根据Pillsbury法将136例对象分成对照组、轻度痤疮组、中度痤疮组和重度痤疮组。然后利用流式细胞仪检测Th17细胞在各组间的表达量；采用ELISA方法检测血清中IL-17、IL-23、IL-6、转化生长因子（TGF）-β1的表达水平，最后通过RTPCR方法分别测定孤独核受体γt(RORγt) mRNA和IL-17 mRNA表达水平。结果: 实验结果表明，中重度痤疮患者血清中IL-17、IL-23的表达量明显高于对照组和轻度痤疮患者（P<0.05），中重度痤疮组的IL-6、TGF-β1的表达量与对照组相比差异有统计学意义（P<0.05），中度痤疮组IL-6、TGF-β1的表达量与轻度痤疮患者差异无统计学意义（P>0.05）；此外，IL-17 mRNA和RORγt mRNA的表达在各组间均存在统计学差异（P<0.05）。结论: Th17细胞在轻度痤疮、中度痤疮和重度痤疮中有级联放大作用，这可为今后重度痤疮探索治疗提供新靶点。

关键词: Th17细胞, 痤疮, IL-17, IL-23, IL-6, TGF-β1, 孤独核受体γt

Abstract:

AIM: To investigate the mechanism of Th17 cells in acne development and its correlation with severity. METHODS: One hundred and thirty six cases were divided into control, mild, moderate and severe acne group according to the Pillsbury method. The expression levels of IL-17, IL-23, IL-6 and TGF-beta1 in serum were detected by ELISA. RORγt mRNA and IL-17 mRNA expression levels were detected by RT-PCR. RESULTS: The levels of IL-17 and IL-23 in serum of patients with moderate to severe acne were significantly higher than those of the control and mild acne groups (P<0.05). The levels of IL-6 and TGF-beta1 in moderate and severe acne groups were significantly higher than those of control group (P<0.05). There was no significant difference in the expression of IL-6 and TGF-beta1 between mild and moderate acne groups (P>0.05). In addition, the expressions of IL-17 mRNA and RORγt mRNA from mild to severe groups were significantly different from control group (P<0.05). And there was significant difference between the three groups (P<0.05). CONCLUSION: Th17 cells have a cascade effect in mild, moderate and severe acne groups, which can provide a new target for future treatment of severe acne.

Key words: Th17 cells, acne, IL-17, IL-23, IL-6, TGF-β1, RORγt

中图分类号:

R751.05

陆威，周炳荣，汤怡，樊一斌，陶小华. Th17细胞在痤疮发病中的作用及与痤疮严重度相关性的功能研究[J]. 中国临床药理学与治疗学, 2018, 23(1): 83-87.

LU Wei, ZHOU Bingrong,TANG Yi, FAN Yibin, TAO Xiaohua. Role of Th17 cells in acne pathogenesis and the correlation with severity of acne[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(1): 83-87.

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Th17细胞在痤疮发病中的作用及与痤疮严重度相关性的功能研究

Role of Th17 cells in acne pathogenesis and the correlation with severity of acne

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