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中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (5): 536-540.doi: 10.12092/j.issn.1009-2501.2018.05.009

• 基础研究 • 上一篇    下一篇

甲基苯丙胺依赖对大鼠纹状体RGS4和mGluR5受体信号传导的影响

赵林波1,2,隋念含2,李利华3,洪仕君3,邢豫明4,赵永娜2   

  1. 1 云南中医学院信息技术学院,昆明 650500,云南; 2 昆明医科大学药学院暨云南省天然药物药理重点实验室,昆明 650500,云南; 3 昆明医科大学法医学院,昆明 650500,云南; 4 云南省公安厅禁毒处毒品技术公安部重点实验室,昆明 650228,云南
  • 收稿日期:2017-11-17 修回日期:2018-01-03 出版日期:2018-05-26 发布日期:2018-05-16
  • 通讯作者: 赵永娜,女,教授,硕士生导师,研究方向:神经药理学。 Tel:15887838797 E-mail:mszhaoyn@163.com
  • 作者简介:赵林波,女,硕士,助教,研究方向:神经药理学。 Tel:13529198070 E-mail:82925224@qq.com
  • 基金资助:

    云南省应用基础研究计划联合专项基金资助项目(2012FB023)

Methamphetamine dependent influenced RGS4 and mGluR5 receptor signaling in striatum of rats

ZHAO Linbo 1,2,SUI Nianhan2,LI Lihua3,HONG Shijun3, XING Yuming 4, ZHAO Yongna 2   

  1. 1 School of Institute of Information Technology, Yunnan University Of TCM, Kunming 650500,Yunnan, China;2 School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Product,Kunming Medical University,Kunming 650500,Yunnan, China; 3 School of Forensic Medicine,Kunming Medical University,Kunming 650500,Yunnan, China; 4 Key Laboratory of Narcotics Technology and Ministry of Police of Yunnan Provincial Police Department,Kunming 650228,Yunnan, China
  • Received:2017-11-17 Revised:2018-01-03 Online:2018-05-26 Published:2018-05-16

摘要:

目的:研究甲基苯丙胺依赖不同时间对大鼠脑纹状体内G蛋白信号调节因子4(regulator of G-protein signaling 4,RGS4 )和代谢型谷氨酸受体5 (metabotropic receptor 5,mGluR5)信号传导的影响。方法:建立大鼠甲基苯丙胺(methamphetamine,METH)依赖一周组(1W)、两周组(2W)模型,Western blot技术检测大鼠纹状体内RGS4、mGluR5、G蛋白α亚单位蛋白Q(Gαq)、磷脂酶C-β1(phospholipase C-β,PLCβ1)表达水平。 结果:METH依赖1W、2W组伴药箱停留时间比生理盐水对照组增加,提示METH依赖模型建成。模型组大鼠纹状体内RGS4蛋白表达水平较生理盐水对照组相比明显下调,而模型组mGluR5蛋白和Gαq蛋白、PLCβ1蛋白表达与生理盐水对照组比较均有不同程度上调,且2W组较1W组改变更明显。结论:METH给药可能导致大鼠脑纹状体内RGS4表达下调,mGluR5表达上调,还可使与mGluR5偶联的Gαq、PLCβ1表达均出现不同程度上调。且METH依赖时间越长,RGS4、mGluR5、 Gαq、PLCβ1的表达水平变化越显著。

关键词: 甲基苯丙胺, 纹状体, 条件位置偏爱, G蛋白信号调节因子4, 代谢型谷氨酸受体5, G蛋白α亚单位蛋白Q, 磷脂酶C-β1

Abstract:

AIM: To investigate whether regulator of G-protein signaling 4(RGS4 ) and metabotropic receptor 5 (mGluR5) expression level changed in striatum of the methamphetamine(METH)-dependent rat and whether the changes were related to the time of METH treatment. METHODS: METH-dependent rat model of one week and two weeks were established. The expression levels of RGS4, mGluR5, Galfaq, PLCbeta1 were detected by Western blot analysis. RESULTS: Rats treated with METH of one week and two weeks spent much more time in the METH-paired chamber than NS control group, suggested that METH-dependent model has successfully established. Compared with NS control group, the expression level of RGS4 in the striatum of rats in model group was down-regulated remarkably. Furthermore, the expression level of mGluR5 and its coupling protein Galfaq, phospholipase C-beta1(PLCbeta1) were up-regulated in different degrees when compared with NS control group, and  the change is more remarkable in two weeks group than that of one week group. CONCLUSION: METH treatment down regulate the RGS4 and up regulate mGluR5 in rat striatum, and the expression level of mGluR5 coupling protein Galfaq, PLCbeta. And the longer the time of METH-dependent, the more significant the changes of RGS4, mGluR5, Galfaq, and PLCbeta1.

Key words: methamphetamine, striatum, CPP, RGS4, mGluR5, Galfaq, PLCbeta1

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