关键词: 胡黄连苷, MCF-7, 自噬, PI3K/AKT/mTOR

Abstract:

AIM: To investigate the effects of PicrosideII on autophagy and PI3K/AKT/mTOR pathway in MCF-7 breast cancer cells. METHODS: Fifty nude mice were randomly divided into five groups, ten in each group, divided into model group, 3-MA group, high dose (100 mg/kg), medium dose (10 mg/kg), low dose (1 mg/kg). Human breast cancer cells MCF-7 were cultured in vitro, and then transplanted into nude mice to establish a subcutaneous transplantation model of MCF-7 breast cancer. The weight of each group of nude mice and the breast tumor volume were measured, the tumor inhibition rate was calculated. Tumor cell morphology was observed by HE staining, and the apoptosis of MCF-7 cells by TUNEL assay.Western blot was used to detect the expression of Beclin1, PI3K, p-PI3K, AKT, p-AKT, mTOR and p-mTOR proteins. RESULTS:PicrosideII significantly inhibited the weight loss of breast cancer nude mice (P<0.01), increased the tumor inhibition rate (P<0.01), improved the pathological tissue results; promoted the apoptosis of MCF-7 cells, increased the expression of Beclin1 protein, and reduced PI3K expression of p-PI3K, AKT, p-AKT, mTOR, p-mTOR protein (P<0.01). CONCLUSION: PicrosideII has a significant inhibitory effect on MCF-7 breast cancer cells, and its mechanism is related to inhibition of PI3K/AKT/mTOR pathway and enhancement of autophagy in MCF-7 cells.

Key words: PicrosideII, MCF-7, autophagy, PI3K/AKT/mTOR