SNI小鼠中调控ROS对疼痛和脊髓Pink1蛋白的影响及机制研究

doi:10.12092/j.issn.1009-2501.2019.11.008

中国临床药理学与治疗学 ›› 2019, Vol. 24 ›› Issue (11): 1263-1268.doi: 10.12092/j.issn.1009-2501.2019.11.008

SNI小鼠中调控ROS对疼痛和脊髓Pink1蛋白的影响及机制研究

蔡妙国¹，邵卫¹，俞慧君²，洪叶¹，施莉莉³

¹台州医院路桥院区肿瘤内科，台州 318050，浙江；²台州医院路桥院区儿科，台州 318050，浙江；³台州医院路桥院区感染科，台州 318050，浙江

收稿日期:2019-07-01 修回日期:2019-08-15 出版日期:2019-11-26 发布日期:2019-12-02
通讯作者: 邵卫，男，本科，主治中医师，研究方向：肿瘤内科。 Tel：0576-82421047 E-mail：jdzx02@qq.com
作者简介:蔡妙国，男，本科，副主任医师，研究方向：肿瘤内科。 Tel：15857660676 E-mail：xs3b9j@sina.com
基金资助:
浙江省中医药科学研究基金项目（2016ZA203）

Effects of regulation ROS on the pain and the protein of Pink1 in spinal of SNI mice and its mechannism

CAI Miaoguo¹, SHAO Wei¹, YU Huijun², HONG Ye¹, SHI Lili ³

¹ Department of Oncology, Luqiao Branch of Taizhou Hospital, Taizhou 318050, Zhejiang, China; ²Department of Pediatrics, Luqiao Branch of Taizhou Hospital, Taizhou 318050, Zhejiang, China; ³ Infection Department of Luqiao Hospital, Taizhou Hospital, Taizhou 318050, Zhejiang, China

Received:2019-07-01 Revised:2019-08-15 Online:2019-11-26 Published:2019-12-02

摘要/Abstract

摘要：

目的:观察C57/BL6小鼠坐骨神经分支选择性损伤（spared nerve injury,SNI）后降低氧化应激反应对机械痛和脊髓中Pink1的表达影响，并探讨神经性疼痛中氧化应激对Pink1的可能的作用机制。方法:取60只小鼠，随机分为对照组（Control）、假手术组（Sham）、手术组（SNI）、SNI+Saline、SNI+苯亚甲基叔丁基氮氧化物（phenyl-N-tert-butylnitrone，PBN）5组。Control组不作任何处理，Sham组切开皮肤，分离出坐骨神经三支分支后缝合皮肤；SNI组游离并保留腓肠神经分支，结扎并切断胫神经；分别于14 d之后进行机械痛阈值检测。测定行为学后SNI组分别注射生理盐水（0.9%NaCl）和PBN。进行行为学测定，并分别检测氧化应激（GSH、SOD）水平；Western blot法检测腰段脊髓蛋白水平Pink1的表达变化。结果:SNI组与Control和Sham组相比，机械痛阈值显著降低，差异具有统计学意义（P<0.001）；SNI组腰段脊髓ROS水平升高（P<0.05），注射PBN后ROS水平降低（P<0.001）；SNI小鼠中Pink1在蛋白水平表达增多（P<0.001），降低ROS后脊髓中Pink1在蛋白水平表达降低（P<0.01）。结论:神经病理痛中降低线粒体内ROS水平则会改善小鼠神经性疼痛，靶向减少ROS的产生和改善Pink1有助于神经病理痛的治疗。

关键词: 神经分支选择性损伤, 线粒体, Pink1, ROS, 小鼠

Abstract:

AIM: To observe the effect of oxidative stress on the expression of Pink1 in mechanical pain and spinal cord after C57/BL6 mice with spared nerve injury (SNI), and to explore the possible effects and mechanism of oxidative stress on Pink1 in neuropathic pain. METHODS: Sixty mice were randomly divided into Control group, Sham group (Sham), Surgery group (SNI), SNI+Saline, and SNI+phenyl-N-tert-butylnitrone (PBN). The Control group received no treatment. The Sham group was cut the skin and separated the three branches of the sciatic nerve to suture the skin. The SNI group was freed and retained the branches of the sacral nerve, and ligated and cut the phrenic nerve. The mechanical pain threshold was detected after 14 days. After the behavioral test, the SNI group was injected with 0.9% physiological saline and PBN. Behavioral measurements were performed and oxidative stress (GSH, SOD) levels were measured. Western blot was used to detect the expression of Pink1 in the lumbar spinal cord protein in each group. RESULTS:Compared with the Control and Sham groups, the mechanical pain threshold was significantly lower in the SNI group (P<0.001); In the SNI group, the ROS level in the lumbar spinal cord was increased (P<0.05), and the ROS level was decreased after PBN injection (P<0.001); Pink1 expression was increased at the protein level in SNI mice (P<0.001), and the expression of Pink1 in the spinal cord decreased after ROS decreased (P<0.01). CONCLUSION:Pink1 is highly expressed in the protein level in the spared nerve injury, and reduced ROS level can improve the expression of Pink1 in neuropathic pain of mice.

Key words: spared nerve injury, mitochondria, Pink1, ROS, mice

中图分类号:

R965.2
R741

蔡妙国，邵卫，俞慧君，洪叶，施莉莉. SNI小鼠中调控ROS对疼痛和脊髓Pink1蛋白的影响及机制研究[J]. 中国临床药理学与治疗学, 2019, 24(11): 1263-1268.

CAI Miaoguo, SHAO Wei, YU Huijun, HONG Ye, SHI Lili. Effects of regulation ROS on the pain and the protein of Pink1 in spinal of SNI mice and its mechannism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(11): 1263-1268.

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SNI小鼠中调控ROS对疼痛和脊髓Pink1蛋白的影响及机制研究

Effects of regulation ROS on the pain and the protein of Pink1 in spinal of SNI mice and its mechannism

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