利多卡因对骨肉瘤细胞MG-63恶性增殖、侵袭及线粒体呼吸作用的调节

doi:10.12092/j.issn.1009-2501.2020.05.006

摘要/Abstract

摘要： 目的:探讨利多卡因对骨肉瘤细胞MG-63恶性增殖、侵袭及线粒体呼吸作用的影响。方法:采用25、50、100 μmol/L剂量利多卡因处理MG-63细胞,将细胞随机分为四组:Lidocaine 0 μmol/L组、Lidocaine 25 μmol/L组、Lidocaine 50 μmol/L组和Lidocaine 100 μmol/L组进行后续实验。BrdU染色检测细胞增殖;Transwell检测细胞侵袭;蛋白免疫印迹检测Ki67、Survivin、血管表皮生长因子(VEGF)、Vimentin蛋白表达水平;流式分选仪检测线粒体膜电位;Clark氧电极法检测线粒体呼吸复合物活性;试剂盒检测三磷酸腺苷(ATP)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果:结果表明,与Lidocaine 0 μmol/L组相比较,Lidocaine 50、100 μmol/L组BrdU阳性细胞数显著降低(P<0.05),侵袭细胞数显著降低(P<0.05),Ki67、Survivin、VEGF、Vimentin蛋白水平显著降低(P<0.05),线粒体膜电位明显降低,复合物I、II、IV活性显著降低(P<0.05),ATP、SOD含量显著降低(P<0.05),MDA含量显著升高(P<0.05)。结论:利多卡因具有抑制骨肉瘤细胞MG-63恶性增殖、侵袭和改善线粒体功能的作用。

关键词: 利多卡因, 骨肉瘤, 恶性生物学行为, 线粒体呼吸

Abstract: AIM: To investigate the effects of lidocaine on malignant proliferation, invasion and mitochondrial respiration of osteosarcoma MG-63 cells. METHODS: MG-63 cells were treated with 25, 50 and 100 μmol/L of lidocaine and were randomly divided into four groups: lidocaine 0 μmol/L, lidocaine 25 μmol/L, lidocaine 50 μmol/L and lidocaine 100 μmol/L for subsequent experiments. BrdU staining was used to detect cell proliferation. Transwell for cell invasion. Protein expression levels of Ki67, Survivin, VEGF and Vimentin were detected by Western blot. Mitochondrial membrane potential was detected by flow separator. Activity of mitochondrial respiratory complex was detected by Clark oxygen electrode method. The kit detected the content of ATP, SOD and MDA. RESULTS: Results showed that compared with lidocaine 0 μmol/L group, BrdU positive cells in lidocaine 50, 100 μmol/L group was significantly reduced (P<0.05), invasive cells was significantly reduced (P<0.05), Ki67, Survivin, VEGF, Vimentin protein levels decreased significantly (P<0.05), mitochondrial membrane potential decreased significantly, compound I, II, IV activity decreased significantly (P<0.05), ATP, SOD content decreased significantly (P<0.05), MDA content was significantly increased (P<0.05). CONCLUSION: Lidocaine can inhibit the malignant proliferation, invasion and improve the mitochondrial function of osteosarcoma MG-63 cells.

Key words: lidocaine, osteosarcoma, malignant biological behavior, mitochondrial respiration

中图分类号:

R738.1

陈益, 曹华, 蔡玲芳, 陈本, 陈明坤. 利多卡因对骨肉瘤细胞MG-63恶性增殖、侵袭及线粒体呼吸作用的调节[J]. 中国临床药理学与治疗学, 2020, 25(5): 519-526.

CHEN Yi, CAO Hua, CAI Lingfang, CHEN Ben, CHEN Mingkun. Lidocaine inhibits the malignant proliferation, invasion and regulates the mitochondrial respiration of osteosarcoma MG-63 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(5): 519-526.

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