益气活血汤通过miR-21/PTEN信号通路抑制肺癌细胞恶性生物学行为

doi:10.12092/j.issn.1009-2501.2020.05.005

摘要/Abstract

摘要： 目的:探讨益气活血汤(Yiqi Huoxue decoction)对肺癌细胞恶性生物学行为的抑制作用及其机制。方法:以不同浓度益气活血汤含药血清(5%、10%、15%)处理人肺癌A549细胞,CCK-8(Cell Counting Kit-8)法、Transwell小室实验、流式细胞术、Western blot法和定量逆转录聚合酶链式反应(qRT-PCR)法研究益气活血汤含药血清对细胞增殖、迁移和侵袭能力、细胞凋亡、第10染色体同源丢失性磷酸酶-张力蛋白(phosphatase and tensin homolog deleted on chromosome ten, PTEN)蛋白和miR-21表达的影响。结果:与无药血清组相比较,不同剂量的益气活血汤含药血清处理后,A549细胞存活率降低、迁移和侵袭能力下降;细胞凋亡率上升、细胞内PTEN mRNA及其蛋白表达增加、miR-21表达下降,且10%含药血清组的效果最佳。转染miR-21 mimics后,A549细胞中miR-21表达上调,PTEN蛋白表达下调;10%含药血清处理后PTEN蛋白表达上调。结论:益气活血汤可有效抑制人肺癌A549细胞恶性细胞生物学行为,且可能与miR-21/PTEN信号通路的调控有关。

关键词: 益气活血汤, miR-21/PTEN信号通路, 肺癌细胞, 恶性生物学行为, 抑制作用, 机制

Abstract: AIM: To investigate the inhibitory effect of Yiqi Huoxue decoction on the malignant biological behavior of lung cancer cells and its mechanism. METHODS: Human lung cancer A549 cells were treated with different doses of Yiqi Huoxue Decoction serum (5%, 10%, 15%). CCK-8 assay, transwell chamber experiment, flow cytometry, Western blot and qRT-PCR method were used to study the effect of different doses of Yiqi Huoxue Decoction-containing serum to cell proliferation, cell migration and invasion, cell apoptosis, PTEN protein and miR-21 expression. RESULTS: Compared with the drug-free serum group, survival rate, migration and invasion ability of A549 cells decreased after treatment with different doses of drug-containing serum. The apoptosis rate of A549 cells increased, PTEN mRNA and the expression of its protein increased, the expression of miR-21 decreased, and the medium-dose (10%) drug-containing serum group had the best effect. After the transfection of miR-21 mimics, miR-21 expression was up-regulated, while PTEN protein expression was down-regulated in cells. PTEN protein expression was up-regulated after treatment with medium-dose (10%) drug-containing serum. CONCLUSION: Yiqi Huoxue Decoction can effectively inhibit the malignant cell biological behavior of human lung cancer A549 cells and may be related to the regulation of the miR-21/PTEN signaling pathway.

Key words: Yiqi Huoxue decoction, miR-21/PTEN signaling pathway, lung cancer cells, malignant biological behavior, inhibitory effect, mechanism

中图分类号:

R965.2

杨国良, 胡丹丹, 徐一凯, 楼黎明. 益气活血汤通过miR-21/PTEN信号通路抑制肺癌细胞恶性生物学行为[J]. 中国临床药理学与治疗学, 2020, 25(5): 512-518.

YANG Guoliang, HU Dandan, XU Yikai, LOU Liming. Yiqi Huoxue decoction inhibits malignant biological behavior of human lung cancer cells via the miR-21/PTEN signaling pathway[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(5): 512-518.

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