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中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (11): 1211-1215.

• 基础研究 • 上一篇    下一篇

非诺贝特抑制血管紧张素Ⅱ所诱导的心脏成纤维细胞骨桥蛋白表达升高

李文举, 石苗茜, 欧东波, 丁璐, 牛晓琳, 刘雄涛, 郑强荪   

  1. 第四军医大学唐都医院心内科,西安 710038,陕西
  • 收稿日期:2010-08-16 修回日期:2010-10-12 出版日期:2010-11-26 发布日期:2020-09-16
  • 通讯作者: 郑强荪,男,教授,主任医师,博士研究生导师,研究方向:心衰的机制与临床研究。Tel: 029-84777422 E-mail: qiangsunzheng@gmail.com
  • 作者简介:李文举,男,硕士研究生,研究方向:心衰的机制与临床研究。Tel: 15829739527 E-mail: adrianli@foxmail.com

Inhibition of fenofibrate on angiotensinⅡ-induced high expression of osteopontin in cardiac fibroblasts

LI Wen-ju, SHI Miao-qian, OU Dong-bo, DING Lu, LIU Xiao-lin, NIU Xiong-tao, ZHENG Qiang-sun   

  1. Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi, China
  • Received:2010-08-16 Revised:2010-10-12 Online:2010-11-26 Published:2020-09-16

摘要: 目的: 探讨过氧化物酶体增殖物激活受体α(PPARα)激动剂非诺贝特对血管紧张素Ⅱ(AngII)诱导的心脏成纤维细胞骨桥蛋白表达升高的影响。方法: 分离并传代培养SD大鼠乳鼠心脏成纤维细胞,使用不同浓度(0、25、50、100 μmol/L)的非诺贝特预处理 1 h 后,加入AngII作用 24 h。分别采用实时定量PCR法和Western blotting技术检测骨桥蛋白mRNA和蛋白表达情况。结果: 非诺贝特显著抑制AngII诱导的心脏成纤维细胞骨桥蛋白mRNA及蛋白的表达,并且呈剂量依赖性。结论: 非诺贝特对心脏成纤维细胞中骨桥蛋白的表达具有明显的抑制作用,这可能是其在心血管系统发挥抗纤维化和抗炎作用的部分机制。

关键词: 心肌纤维化, 心脏成纤维细胞, 非诺贝特, 骨桥蛋白

Abstract: AIM: To investigate the effects of fenofibrate, peroxisome proliferator-activated receptor α (PPARα) agonist, on the up-regulation of angiotensin II (Ang II)-induced osteopontin in rat cardiac fibroblasts. METHODS: Isolated cardiac fibroblasts of neonatal Sprague-Dawley rat were pretreated with various concentrations of fenofibrate (0, 25, 50 and 100 μmmol/L) for 1 hour. Then Ang II were added into cells for 24 hours. The expression of osteopontin was measured by real-time quantitative RT-PCR and Western blotting, separately. RESULTS: Significant inhibitory effects of fenofibrate on Ang II-induced high expression of osteopontin were observed in cardiac fibroblasts and the effect was dose dependent (P<0.05). CONCLUSION: The present data suggests that fenofibrate can down-regulate the level of osteopontin in rat cardiac fibroblasts, which may explain the anti-inflammatory and anti-fibrosis effects of fenofibrate in the cardiovascular system.

Key words: Myocardial fibrosis, Cardiac fibroblast, Fenofibrate, Osteopontin

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