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中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (9): 983-985.

• 基础研究 • 上一篇    下一篇

姜黄素保护刀豆蛋白A诱导的小鼠肝损伤作用的研究

陈丽华, 肖新宇, 李子博, 程杨艳   

  1. 长沙医学院检验系,长沙 410219,湖南
  • 收稿日期:2010-03-11 修回日期:2010-04-24 出版日期:2010-09-26 发布日期:2020-09-17
  • 作者简介:陈丽华,女,硕士,研究方向:免疫药理。Tel: 0731-88498197, E-mail: chenlihuacc@163.com
  • 基金资助:
    长沙医学院院级课题(20082747)

Protective effect of curcumin on Con A-induced liver injury in mice and its mechanism

CHEN Li-hua, XIAO Xin-yu, LI Zi-bo, CHENG Yang-yan   

  1. Department of Clinical Laboratory Medicine, Changsha Medical University, Changsha 410219, Hunan, China
  • Received:2010-03-11 Revised:2010-04-24 Online:2010-09-26 Published:2020-09-17

摘要: 目的: 研究姜黄素(curcumin, Cur)对刀豆蛋白A(concanavalin A, Con A)诱导的小鼠肝损伤的保护作用及可能机制。方法: 40只ICR小鼠被随机均分为正常对照组、模型组、Cur 100 mg/kg 组和 200 mg/kg 组。受试物组每天灌胃姜黄素液,模型组和正常对照组每天灌胃同体积生理盐水,连续 10 d。末次给药后 4 h,除正常对照组外均采用尾静脉注射Con A 20 mg/kg,8 h 后检测被测试动物肝匀浆液的丙二醛(MDA)和超氧化物歧化酶(SOD)指标及血清ALT、AST指标,观察肝组织病理学改变。结果: 与模型组相比,受试物组ALT、AST活力均显著降低,肝脏MDA水平降低,SOD活力提高,肝组织病理改变减轻。结论: 姜黄素具有减轻肝损伤的作用,该作用可能通过减轻肝脏的氧化应激损伤来实现。

关键词: 姜黄素, 肝损伤, 刀豆蛋白A, 氧化应激

Abstract: AIM: To investigate the protective effect of curcumin(Cur) on concanavalin A(Con A)-induced liver injury in mice and the potential mechanism.METHODS: Forty ICR mice were randomly divided into 4 groups:normal control group,model group,Cur 100 mg/kg group and Cur 200 mg/kg group. Cur 100 mg/kg group and Cur 200 mg/kg group were administrated with Cur at the dosage of 100 mg/kg and 200 mg/kg via intragastric respectively for 10 days continuously. Normal control group and model group were accepted with the same volume of normal saline by the same means. In the 4th hour after the last administration,the mice were injected with Con A at the dosage of 20 mg/kg via the tail vein . The levels of ALT and AST in serum and the activity or content of superoxide dismutase (SOD) and ma1ondialdehtyde(MDA) in liver were measured in the 8th hour after injection of Con A, and the histopathological examination was also performed for liver tissue.RESULTS: Compared with the model group, Cur significantly down-regulated the levels of ALT, AST and MDA.Inversely, the activity of SOD was elevated. Hepatic histopathological changes were alleviated.CONCLUSION: Cur has protective effect on Con A-induced liver injury.This may be implemented by inhibiting oxidative stress.

Key words: Curcumin, Liver injury, Concanavalin A, Oxidative stress

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