复方丹参注射液对大鼠体外循环后肺损伤的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (9): 1039-1042.

复方丹参注射液对大鼠体外循环后肺损伤的影响

张丕兴, 林丽丽

青岛市第八人民医院, 青岛 266100, 山东

收稿日期:2006-04-10 修回日期:2006-05-19 出版日期:2006-09-26 发布日期:2020-11-05
通讯作者: 张丕兴, 男, 副教授, 大学本科, 研究方向:脏器功能保护。Tel:0532-87080880 　E-mail:zpx5872210@163.com

Effects of compound salvia miltiorrhiza injection on lung injury after cardiopulmonary bypass in rats

ZHANG Pi-xing, LIN Li-li

Department of Anesthesiology , the Eighth People Hospital of Qingdao, Qingdao 266100 , Shandong, China

Received:2006-04-10 Revised:2006-05-19 Online:2006-09-26 Published:2020-11-05

摘要/Abstract

摘要： 目的研究大鼠体外循环(CPB) 后肺组织损伤以及复方丹参注射液对肺损伤的影响。方法 24只SD 大鼠随机分为3 组, 对照组、CPB 组和丹参组,每组8 只。CPB 组和丹参组建立体外循环, 丹参组为停循环前5 min 经体外循环的储血器给入复方丹参注射液1 ml·kg^-1体重。对照组为假手术对照。CPB 后60 min 取部分肺组织, 测定并计算丙二醛(MDA) 、髓过氧化物酶(MPO) 和组织含水量。取部分肺组织用多聚甲醛固定, 制作石蜡切片, HE 染色后观察形态学改变。结果光学显微镜下观察可见,CPB 组肺组织间质增厚、细胞浸润, 丹参组的程度较CPB 组有所减轻。CPB 组和丹参组肺组织中MDA含量、MPO 含量和含水量均明显高于对照组(p<0.05) , 丹参组升高程度小于CPB 组(p<0.05) 。结论体外循环过程中应用复方丹参注射液能有效减轻肺损伤程度, 可能与其清除氧自由基、减轻中性粒细胞浸润等作用有关。

关键词: 体外循环, 肺损伤, 复方丹参注射液, 大鼠

Abstract: AIM: To investigate the effects of compound salvia miltiorrhiza (SM) injection on lung injury after cardiopulmonary bypass (CPB).METHODS: Twenty-four rats were randomly divided into control group, CPB groupand SM group.Cardiopulmonary bypass was made in rats of CPB groupand SM group.Lung tissues of all rats were taken 60 min after the weaning of CPB for the assay of malondialdehyde (MDA) , myeloperoxidase(MPO) , water content of tissue, the observation of pathological changes were made under optical microscope.RESULTS: Remarkable pathohistological and biochemical changes were detected 1 h after CPB.Compared with control group, MDA in lung tissue were increased in CPB groupand SM group(p<0.05) , but MDA in SM groupwas lower than that in CPB group(P<0.05).Similarly, the level of MPO and water content were increased markedly compared with control groupand SM group(p<0.05).Accordingly , swelling and infiltration of leucocyte were found in lung tissue of CPB groupunder an optical microscope.These pathohistological damages caused by CPB were greatly attenuated in SM group.CONCLUSION: CPB-related lung injury can be reduced by compound salvia miltiorrhiza injection which has free radical scavenging and neutrophil infiltration relieving activities.

Key words: cardiopulmonary bypass, lung injury, compound salvia miltiorrhiza injection, rats

中图分类号:

R965.2

张丕兴, 林丽丽. 复方丹参注射液对大鼠体外循环后肺损伤的影响[J]. 中国临床药理学与治疗学, 2006, 11(9): 1039-1042.

ZHANG Pi-xing, LIN Li-li. Effects of compound salvia miltiorrhiza injection on lung injury after cardiopulmonary bypass in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(9): 1039-1042.

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