新的尖吻蝮蛇蛇毒类凝血酶Agacutin止血作用的实验研究

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (7): 781-786.

新的尖吻蝮蛇蛇毒类凝血酶Agacutin止血作用的实验研究

唐松山, 王晓华, 张娟辉, 杨志勇, 刘菁, 祁荣

广东药学院微生物与生化药学教研室, 生物科学与生物制药学院, 广州510006, 广东

收稿日期:2005-06-14 修回日期:2005-06-25 出版日期:2005-07-26 发布日期:2020-11-10
通讯作者: 唐松山, 男, 博士, 讲师, 研究方向:生化药学。Tel:(0) 13113315075 E-mail: tssjx2003@yahoo.com.cn

Experimental study on hemostatic effects of agacutin, a novel thrombinlike enzyme from Agkistrodon acutus

TANG Song-shan, WANG Xiao-hua, ZHANG Juan-hui, ZHANG Yuan, LEI Tian-luo, BU Xiu-yun

Department of Microbiological and Biochemical Pharmacy, Bioscience and Biopharmaceutical College, Guangdong Pharmaceutical College, Guangzhou 510224, Guangdong, China

Received:2005-06-14 Revised:2005-06-25 Online:2005-07-26 Published:2020-11-10

摘要/Abstract

摘要： 目的: 研究类凝血酶Agacutin 的止血效果。方法: 新西兰白兔静脉注射Agacutin, 观察血凝和纤溶指标;小鼠皮下注射Agacutin, 采用小鼠剪尾试验测定其止血效果。结果: 静脉注射Agacutin 0.01 ~0.05 U·kg^-1后, 各组全血凝固时间与给药前相比均有不同程度的缩短(44.9 % ~ 60.5 %), 给药后30 min 药效达高峰, 药效持续24 h 。与给药前比较,各组兔血纤维蛋白原含量和血粘度在给药后有不同程度的降低, 这与血液凝固时间缩短一致。试验结果显示, Agacutin 对部分凝血活酶时间、血小板数量、血小板释放活性、血小板体外聚集、优球蛋白凝固时间均无明显影响, 但对优球蛋白溶解时间有不同的缩短, 显示有弱的溶栓活性。腹腔注射Agacutin0.5 ~ 2 U·kg^-1剂量后, 小鼠剪尾出血时间有不同程度的缩短(24 %~ 66 %) 。结论: Agacutin 在血管内引起较高浓度的可溶性纤维蛋白且不激活凝血因子II和XIII, 在伤口部位加速止血, 在正常的血管内, 不会造成血栓形成。

关键词: 类凝血酶, 止血剂, 尖吻蝮蛇蛇毒, Agacutin

Abstract: AIM: To study the hemostatic effects of agacutin in vivo. METHODS: After agacutin was administrated, the indexes on blood coagulation and fibrinogenlysis system were assayed and the hemostatic effect was obtained by observing the mouse tail bleeding time. RESULTS: The intravenous injection of 0.01 - 0.05 U·kg^-1 markedly shortened 44.9 %-60.5 %of the rabbit blood coagulation time. After administration, the peak effect appeared in 30 min and the effect delayed for 24 h. The rabbit blood fibrinogen concentration and blood viscosity were decreased, which coincided with the effect of shortening of blood coagulation time. Agacutin had no influence on APTT, the platelet count, platelet releasing activity, platelet in vitro aggregation, and euglobulin coagulation time (ECT), but agacutin had a weak fibrinolytic activity. In vivo, the ip administration of 0.5 -2.0 U·kg^-1 shortened 24 %-66 %of the mouse tail bleeding time. CONCLUSION: Agacutin causes a higher concentration of soluble fibrin level in vessel and it does not activate prothrombin (II factor) and XIII factor. The hemostatic function is promoted at the wound. In normal vessel, the soluble fibrin does not form insoluble fibrin clot which resulted in thrombosis in vivo.

Key words: thrombin-like enzyme, coagulant, agkistrodon acutus snake venom, agacutin, pharmacodynamics

中图分类号:

R973.1

唐松山, 王晓华, 张娟辉, 杨志勇, 刘菁, 祁荣. 新的尖吻蝮蛇蛇毒类凝血酶Agacutin止血作用的实验研究[J]. 中国临床药理学与治疗学, 2005, 10(7): 781-786.

TANG Song-shan, WANG Xiao-hua, ZHANG Juan-hui, ZHANG Yuan, LEI Tian-luo, BU Xiu-yun. Experimental study on hemostatic effects of agacutin, a novel thrombinlike enzyme from Agkistrodon acutus[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(7): 781-786.

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