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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (5): 518-521.

• 研究原著 • 上一篇    下一篇

海狗油对实验性脂肪肝脂代谢和血栓素 A2 及丙二醛的影响

李湛军, 刘建文, 徐康森   

  1. 中国药品生物制品检定所生化药品与基因工程药物室, 北京 100050
  • 收稿日期:2005-03-10 修回日期:2005-04-16 出版日期:2005-05-26 发布日期:2020-11-19
  • 通讯作者: 李湛军,女, 医学博士, 副主任药师, 研究方向:生化药理与生物检定。Tel:010-67017755-464 E-mail:zhanjunqq@yahoo.com.cn

Effects of seal oil on liver lipid metabolism and thromboxane A 2 and MDA of experimental fatty liver in rats

LI Zhan-jun, LIU Jian-wen, XU Kang-sen   

  1. Department of Biochemical Drug and Genetic Engineering Pharmaceutical, National Institute for the Control of Pharma-ceutical and Biological Products, Beijing 100050, China
  • Received:2005-03-10 Revised:2005-04-16 Online:2005-05-26 Published:2020-11-19

摘要: 目的: 探讨海狗油抑制脂肪肝的作用机理。方法: 小剂量四氯化碳合并高脂饲料复制大鼠脂肪肝模型 7 周后, 模型组灌胃(ig) 给予等体积橄榄油;辛伐他汀组给予辛伐他汀 4 mg°kg-1°d-1, 海狗油低、中、 高 剂 量 组 ig 海 狗 油 0.5、 1.6、 4.8g°kg-1°d-1, 连续 8 周, 正常大鼠作为空白组。测定肝脂、肝内前列环素的代谢产物(6-keto-PGF) 与血栓素A2 的代谢产物(TXB2) 含量, 观察脂质过氧化作用和组织学检查结果。结果: 海狗油各组肝细胞内TXB 2 含量降低, 6-keto-PGF/TXB2 比值增加, 超氧化物歧化酶(SOD) 活性升高, 甘油三脂(TG)、总胆固醇(TC)、丙二醛(MDA)、游离脂肪酸(FFA) 含量显著降低, 组织学检查显示肝内脂变减轻, 细胞色素P450 2E1(CYP2E1) 免疫组织化学染色显色指数减小。结论: 海狗油使实验性脂肪肝的肝脂和肝内血栓素A2 的代谢产物减少, 即抑制肝脂合成、促进肝脂代谢(直接作用);使肝内血液粘度减少、血液流动性增加(间接作用), 从而对实验性脂肪肝有抑制作用;同时海狗油使 MDA 含量降低、SOD 活性升高而有抗氧化作用。

关键词: 海狗油, 脂肪肝, 肝脂, 血栓素 A2, 前列环素, 丙二醛

Abstract: AIM: To study the inhibition effects of seal oil on fatty liver in rats.METHODS: A low dose of carbon tetrachloride and high fat diet chronically feed was given to five groups (n =10 in each) of male Wistar rats for 7 weeks to induce the model of fatty liver. Then the five groupswere orally administered olive oil, simvastatin 4 mg°kg-1°d-1, seal oil 0.5, 1.6 or 4.8 g°kg-1°d-1 for other 8 weeks, respectively. The untreated control group received only normal feed.The liver lipid, produc-tion of prostacyclin and thromboxane A2, lipid superoxi-dation parameters were measured to compare with the changes.The pathology was evaluated. RESULTS: The ratio of 6-keto-PGF/TXB2 were significantly increased in seal oil group due to decrease TXB2 and increase 6-keto-PGF in liverwhen compared with those in olive oil group (model group) (P<0.01). Liver TC, TG, FFA and MDA concentrations were significantly decreased in the seal oil groupswhen compared with those in model group (P<0.01), but SOD concentration increased (P< 0.01). Pathology showed that seal oil markedly improved fatty liver and the reduction of index analyzed from CYP2E1 express (P<0.01). CONCLUSION: Seal oil inhibits fatty changes of the liver by both direct effects of inhibition the synthesis of liver lipid and modulating lipid metabolism and indirect effects of increase the hepatic blood flow due to decrease the TXA2 concentration and blood viscosity. In addition, seal oil shows anti-lipid su-peroxidation by decreasing MDA concentration.

Key words: seal oil, fatty liver, liver lipid, TXA2, PGI, MDA

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