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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (1): 57-60.

• 研究原著 • 上一篇    下一篇

生存素反义寡核苷酸诱导HL-60 细胞凋亡的实验研究

齐世美, 毕富勇   

  1. 皖南医学院生物化学教研室, 芜湖 241001, 安徽
  • 收稿日期:2004-11-28 修回日期:2004-12-23 出版日期:2005-01-26 发布日期:2020-11-19
  • 通讯作者: 毕富勇, 男, 副教授, 硕士研究生导师, 研究方向:肿瘤生化。Tel:0553-3932262 E-mail:bifuyong@mail.wnmc.edu.cn
  • 作者简介:齐世美, 女, 硕士研究生, 专业方向:肿瘤生化。Tel:0553-3932262 E-mail:juliaqi@163.com
  • 基金资助:
    安徽省科学技术厅重点科研基金项目(No200101023023)

Effects of antisense oligonucleotide targeting survivin on apoptosis in HL-60 cells

QI Shi-mei, BI Fu-yong   

  1. Department of Biochemistry, Wannan Medical College, Wuhu 241001, Anhui, China
  • Received:2004-11-28 Revised:2004-12-23 Online:2005-01-26 Published:2020-11-19

摘要: 目的: 探讨特异性靶向生存素的反义寡核苷酸(ASODN) 对急性早幼粒细胞系HL-60 细胞凋亡的影响。方法: 应用四唑盐(MTT) 比色法分析细胞增殖;倒置显微镜观察细胞形态学变化;原位末端标记(TUNEL) 法检测细胞凋亡指数(AI);流式细胞术(FCM) 定量检测细胞凋亡;RT-PCR 半定量检测生存素mRNA 的表达。结果: 5~20 μmol·L-1生存素ASODN 对HL-60 细胞增殖均有抑制作用, 且呈时间和剂量依赖性。ASODN 组凋亡率和生存素基因表达抑制率明显高于对照组。结论: 生存素ASODN 能有效抑制HL-60 细胞生存素mRNA 的表达并诱导细胞凋亡, 表明生存素对维持肿瘤细胞的增殖具有非常重要的作用。

关键词: 凋亡抑制蛋白, 生存素, 反义寡核苷酸, HL-60 细胞, 细胞凋亡

Abstract: AIM: To explore the effects of ASODN targeting survivin on apoptosis in HL-60 cells.METHODS: The cell proliferation was tested by MTT assay, the cell morphological transformation was observed by inverted microscope, the cell apoptosis index was examined by TUNEL, the cell apoptosis rate was precisely detected by flow cytometry, and the expression of survivin mRNA was detected by reverse transcriptase PCR.RESULTS: 5-20 μmol·L-1of survivin ASODN showed obviously inhibitory effect on the cell proliferation of HL-60 cells, and the inhibitory effect correlated with time and dosage.The cell apoptosis rate and the expression inhibitory rate of survivin gene in ASODN groups were obviously higher than that in the control group.CONCLUSION: ASODN targeting survivin can effectively inhibit the expression of survivinmRNA and induce the cell apoptosis, and it indicates that survivin plays an important role in maintaining the proliferation of tumor cells.

Key words: antisense oligonucleotide, HL-60 cell, apoptosis, inhibitor of apoptosis protein

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