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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (5): 540-543.

• 研究原著 • 上一篇    下一篇

蜕皮甾酮对实验性蛛网膜下腔出血后血管内皮细胞损害的作用研究

陈志, 朱刚, 唐卫华, 刘智, 吴南, 王宪荣, 冯华   

  1. 第三军医大学西南医院神经外科, 重庆400038
  • 收稿日期:2004-01-16 修回日期:2004-03-12 发布日期:2020-11-22
  • 通讯作者: 冯华, 男, 教授, 博士生导师, 研究方向为脑血管病防治和神经创伤修复。Tel:023-68754153 E-mail:fenghua8888 @yahoo.com.cn
  • 作者简介:陈志, 男, 硕士, 主治医师。E-mail:chenzhi@xinhuanet.com

Effects of ecdysterone on injury of endothelial cells following experimental subarachnoid hemorrhage

CHEN Zhi, ZHU Gang, TANG Wei-Hua, LIU Zhi, WU Nan, WANG Xian-Rong, FEN Hua   

  1. Department of Neurosurgery, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China
  • Received:2004-01-16 Revised:2004-03-12 Published:2020-11-22

摘要: 目的 观察血性脑脊液(BCSF) 对培养的微血管内皮细胞(brain microvessel endothelial cells, BmvECs)损害作用和蜕皮甾酮(ecdysterone, EDS) 的干预效应。 方法 将培养的BmvECs 分为对照组、BCSF组和EDS 组, 通过病理形态学改变、细胞计数、MTT比色法和流式细胞仪细胞周期分析评估其损害和增殖状况。 结果 与对照组比较, BCSF 组内皮细胞出现明显的病理形态学改变, 贴壁细胞数量减少, MTT吸光度下降,G0 ~ G1期细胞比例减少。EDS 对BCSF所致上述内皮细胞改变有一定的改善作用。 结论 EDS 对BCSF 所致的内皮形态和增殖损害具有保护作用, 可能在慢性脑血管痉挛治疗中具有一定作用。

关键词: 脑血管痉挛, 蛛网膜下腔出血, 内皮细胞, 蜕皮甾酮

Abstract: AIM: To investigate the effects of ecdysterone (EDS) on injury and proliferation of endothelial cells caused by bloody cerebralspinal fluid (BCSF). METHODS: Rabbit brain microvessel endothelial cells (BmvECs) were divided into control group, BCSF group and EDS group, which were treated with DMEM, BCSF and both of BCSF and EDS (200 mg·L-1), respectively. Morphological changes, cell counting, MTT assay, and flow cytometric analysis were used to evaluate the cytotoxic effects on BmvECs. RESULTS: BCSF could cause severe morphologic changes in BmvECs, decrease the OD of MTT assay and cell proportion of G0-G1 of BmvECs(P <0.01).EDS could alleviate these changes in BmvECs caused by BCSF. CONCLUSION: EDS has protective effects on the injury of BmvECs caused by BCSF, which suggests EDS may play a role in the therapy of CVS.

Key words: cerebral vasospasm, subarachnoid hemorrhage, endothelial cells, ecdysterone

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