全反式维甲酸和阿糖胞苷对HL-60细胞端粒酶活性的影响

中国临床药理学与治疗学 ›› 2000, Vol. 5 ›› Issue (1): 47-48.

全反式维甲酸和阿糖胞苷对HL-60细胞端粒酶活性的影响

吴向华, 叶开和, 胡爱群¹

暨南大学医学院,广州 510630;
¹皖南医学院,芜湖 241001

收稿日期:2000-01-19 出版日期:2000-03-26 发布日期:2020-11-26

Ef fect of the all trans retinoic acid and Ara-con the telomerase activity of HL-60 cells

WU Xiang-Hua, YE Kai-He, HU Ai-Qun¹

Medical college of Jinan University,Gangzhou 510630;
¹Wannan Medical College,Wuhu 241001

Received:2000-01-19 Online:2000-03-26 Published:2020-11-26

摘要/Abstract

摘要： 目的探讨全反式维甲酸(all trans-retinoic acid,ATRA) 和阿糖胞苷(Ara-c) 诱导人早幼粒细胞白血病细胞凋亡和分化的机制。方法采用PCR-ELISA 方法观察A TRA 和Ara-c 对HL-60细胞端粒酶活性的影响。结果 ATRA 和Ara-c 在诱导HL-60 细胞凋亡和分化过程中均可使端粒酶活性减低。结论 ATRA 和Ara-c抑制HK-60 细胞端粒酶的活性,可能是其诱导白血病细胞凋亡和分化的重要机制之一。

关键词: HL-60 细胞, 端粒酶活性, 全反式维甲酸, 阿糖胞苷

Abstract: Aim To investigate the possible mechanism of human promyelocytic leukemia cells differentiation and apoptosis induced by all trans-retinoic acid (ATRA)and A ra-c.Methods The effects of AT RA and Ara-c on the tolomerase activity of HL-60 cells were detected by PCR-E LISA.Results The tolome rase activity of HL-60 cells declined during the course of differentiation and apoptosis induced by AT RA and Ara-c.Conclusion These finding ssuggest that AT RA and Ara-c can inhibit tolomerase activity of HL-60 cells.It may be one of the important mechanisms of human promyelocytic leukemia cells differentiation and apo ptosis induced by ATRA and Ara-c.

Key words: HL-60 cell, telomerase activity, alltrans-re tinonic acid, cytosirearabinoside

中图分类号:

R979.1

吴向华, 叶开和, 胡爱群. 全反式维甲酸和阿糖胞苷对HL-60细胞端粒酶活性的影响[J]. 中国临床药理学与治疗学, 2000, 5(1): 47-48.

WU Xiang-Hua, YE Kai-He, HU Ai-Qun. Ef fect of the all trans retinoic acid and Ara-con the telomerase activity of HL-60 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2000, 5(1): 47-48.

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