欢迎访问《中国临床药理学与治疗学》杂志官方网站,今天是

中国临床药理学与治疗学 ›› 1998, Vol. 3 ›› Issue (4): 250-255.

• • 上一篇    下一篇

咖啡因代谢探针研究乙酰化代谢表型与膀胱癌关系

郭瑞臣, 崔唏, 徐祗顺, 王本杰, 李朝武   

  1. 山东医科大学附属医院,济南 250012
  • 收稿日期:1998-11-02 出版日期:1998-12-26 发布日期:2020-12-01
  • 作者简介:郭瑞臣,男,42岁,山东医科大学临床药理研究所副所长,主任药师,山东医科大学兼职教授,硕士研究生导师,研究方向为临床药理学。

Relationship between bladder cancer and slow N-acetylators phenotyped with caffeine as a probe drug in Chinese population

GUO Rui-Chen, CUI Xi, XU Zhi-Shun, WANG Ben-Jie, LI Chao-Wu   

  1. Affiliated Hospital of Shandong Medical University,Jinan 250012
  • Received:1998-11-02 Online:1998-12-26 Published:2020-12-01

摘要: 目的 以咖啡因为代谢探针,研究膀胱癌发生发展的N-乙酰化代谢表型分布的统计学相关性。方法 根据人尿液中咖啡因代谢物5-乙酰氨基-6-甲酰氨基-3-甲基尿嘧啶(AFMU)和甲磺嘌呤1X)的峰高比值绘制概率分布直方图和概率单位图,寻找区分快慢乙酰化代谢表型的截点,确定人的乙酰化代谢表型分布。结果 健康志愿者和膀胱癌患者概率分布直方图和概率单位图呈明显多态性,截点为1.10,即大于1.10为快乙酰化代谢表型,小于1.10为慢乙酰化代谢表型。健康志愿者中快、慢人乙酰化表型分别为149例(73.7%)和54例(26.3%)。膀胱癌患者分别为36例(53.7%)和31例(46.3),两者存在显著统计学差异(P<0.01)。志愿者和膀胱癌患者基因频率分别为0.51和0.68,优势比为2.376(95%可信区间为1.3513和4.1776)。结论 中国人乙酰化代谢表型分布呈多型性。慢乙酰化代谢表型个体可能为膀胱癌多发和易感人群。

关键词: 乙酰化代谢多态性, 膀胱癌, 咖啡因, 表型

Abstract: Aim Acetylator status of 203 healthy controls and 67 patients with bladder cancer was observed.Methods All the subject s w ere N-acetylate-phenotyped with caffeine asametabolic probe drug.Urine samples were collected 2 hours after a cuPof 140mg-caffeine-spiked coffee was taken under fasting condition in the morning.The caffeine metabolites,5-acetylamino-6-formylamino-1-me thyluracil(AFMU) and 1-methy lxanthine (1X)were analysed by High Performance Liquid Chromatog raphy(HPLC).The frequence histogram and probit plot were constructed to select the antimode which was used to assess slow and fast acetylator statusboth in healthy controls and patients with bladder cancer.Results The peak hight ratios (PHR)of AFMU/1X were from 0.06 to 6.5 for healthy voluteers and 0.1 to 6.31 for patients with bladder cancer.Of the 203 healthy controls involved in this study 73.7%(149/203)had the AFMU/1X>1.10,and were classified as fast acetylators,and 26.3%(54/203)as slow acetylators,while 53.7%(36/67)were fast acetylators and 46.3%(31/67)were slow acetylators in patients with bladder cancer.The oddsratio was 2.376,and the genefreqency for healthy controls and for patients with urinary bladder cancer was 0.51 and 0.68 respectively.Conclusion The acetylator status of Chinese population is polymorphic and completely in concordance with that determined by dapsone,isoniazid or sulfamethazine methords as previously reported.Slow acetylators might be susceptible to urinary bladder cancer.

Key words: N-acetylation, polymorphism, bladder cancer, caffeine

中图分类号: