反相高效液相色谱法测定血浆中的安替比林

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (6): 621-624.

反相高效液相色谱法测定血浆中的安替比林

杨海峰, 胡屹屹, 江善祥

南京农业大学动物医学院药理研究室, 南京 210095, 江苏

收稿日期:2006-03-20 修回日期:2006-04-24 出版日期:2006-06-26 发布日期:2020-12-04
通讯作者: 江善祥,男,教授,研究方向:动物药理与毒理。Tel:025-84396770 E-mail:nauvy@sina.com
作者简介:杨海峰,男,硕士生,研究方向:动物药理与毒理。Tel:025-84395573 E-mail:yhf8142@sina.com

Determination of antipyrine in plasma by reversed phase high-performance liquid chromatography

YANG Hai-feng, HU Yi-yi, JIANG Shan-xiang

Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China

Received:2006-03-20 Revised:2006-04-24 Online:2006-06-26 Published:2020-12-04

摘要/Abstract

摘要： 目的: 建立反相高效液相色谱法(RP-HPLC)检测血浆中安替比林的方法。方法: 血浆经乙腈提取后进样测定, 色谱柱为Kromasil C18 (250 mm×4.6 mm, 5 μm), 柱温30 ℃, 流动相为0.025 mol·L^-1 醋酸钠溶液-乙腈(74:26, v v), 流速为0.8 ml·min^-1, 检测波长为254 nm, 进样量20 μl。结果: 血浆安替比林浓度在0.1~30 μg·ml^-1范围内线性关系良好(r=0.9995), 样品的平均回收率为93.44%~97.21%, 日内和日间变异系数小于4.26%。结论: 方法简便、准确可靠, 适用于安替比林消除动力学模型的研究。

关键词: 安替比林, 反相高效液相色谱法, 血浆药物浓度

Abstract: AIM: To establish a reversed phase highperformance liquid chromatography method for the determination of antipyrine in plasma.Methods: Plasma samples were prepared with acetonitrile.The chromatographic separation was performed on a Kromasil C18 column at column temperature of 30 ℃.The mobile phase, a mixture of 0.025 mol·L^-1 aqueous solution of sodium acetate and acetonitrile (74:26, v v), was delivered at a flow rate of 0.8 ml·min^-1.The detection wavelength was 254 nm and the sample size was 20 μl.Results: Excellent line relationship was obtained in the range of 0.1 to 30 μg·ml^-1 (r=0.9995).The average recovery rate was 93.44%-97.21%.The intra-day and interday coefficients of differentiation were both less than 4.26%.CONCLUTION: The method is simple, accurate and can be used for studies on the model of antipyrine pharmacokinetics.

Key words: antipyrine, reversed phase high-performance liquid chromatography, plasma drug concentration

中图分类号:

R969.1

杨海峰, 胡屹屹, 江善祥. 反相高效液相色谱法测定血浆中的安替比林[J]. 中国临床药理学与治疗学, 2006, 11(6): 621-624.

YANG Hai-feng, HU Yi-yi, JIANG Shan-xiang. Determination of antipyrine in plasma by reversed phase high-performance liquid chromatography[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(6): 621-624.

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