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中国临床药理学与治疗学 ›› 2024, Vol. 29 ›› Issue (11): 1260-1266.doi: 10.12092/j.issn.1009-2501.2024.11.007

• 基础研究 • 上一篇    下一篇

美洲大蠊提取物对前列腺癌细胞的抑制作用及相关机制研究

段赛竹1,许凤1,陈海燕1,成家茂2   

  1. 1大理大学临床医学院,大理  671003,云南;2大理大学基础医学院,大理  671003,云南
  • 收稿日期:2024-05-22 修回日期:2024-07-16 出版日期:2024-11-26 发布日期:2024-10-24
  • 通讯作者: 陈海燕,女,副教授,研究方向:临床疾病超声诊断与基础研究。 E-mail: 316573230@qq.com 成家茂,男,教授,研究方向:肿瘤基础与中医药治疗。 E-mail: 276625041@qq.com
  • 作者简介:段赛竹,女,主治医师,研究方向:前列腺癌的诊断与治疗。 E-mail: 1075900832@qq.com
  • 基金资助:
    云南省教育厅科学研究基金教师类项目(2022J0698)

Research on the inhibitory effect and related mechanisms of Periplaneta americana extract on prostate cancer cells 

DUAN Saizhu1, XU Feng1, CHEN Haiyan1, CHENG Jiamao2   

  1. 1 School of Clinical Medicine, Dali University, Dali 671003, Yunnan, China; 2 School of Basic Medicine, Dali University, Dali 671003, Yunnan, China
  • Received:2024-05-22 Revised:2024-07-16 Online:2024-11-26 Published:2024-10-24

摘要:

目的:研究美洲大蠊提取物(PA-CII-3)抗前列腺癌的作用及相关机制。方法:将对数生长期的PC3细胞分为对照组及实验组,在实验组细胞中依次加入1 000、1 200、1 400、1 600、1 800、2 000 μg/mL的PA-CII-3溶液进行干预,采用CCK-8法筛取合适的药物浓度和作用时间。然后以1 400 μg/mL的PA-CII-3溶液对PC3细胞持续干预72 h,采用CCK-8法和平板集落形成实验检测细胞增殖能力,采用细胞划痕实验检测细胞迁移能力,分别采用Real-time qPCR法和Western blot法检测锌α2糖蛋白(ZAG)的mRNA和蛋白表达情况。结果:PA-CII-3干预后,PC3细胞的增殖和迁移能力均受到显著抑制(P<0.05,P<0.01),并促进了PC3细胞中ZAG的mRNA和蛋白表达(P<0.05)。结论:PA-CII-3可能通过抑制PC3细胞的增殖和迁移及上调ZAG发挥抗前列腺癌作用。

关键词: 美洲大蠊提取物, 前列腺癌, 锌α2糖蛋白, 细胞增殖, 细胞迁移

Abstract:

AIM: To explore the mechanism of Periplaneta americana extracts (PA-CII-3) on anti-prostate cancer. METHODS: Prostate cancer cells PC3 in logarithmic growth phase were divided into a control group and an experimental group. The PA-CII-3 solutions of 1 000, 1 200, 1 400, 1 600, 1 800, and 2 000 μg/mL were added successively to the PC3 cells in experimental group, for screening out the appropriate drug concentration and best time to intervene by CCK-8 assay. And then, PA-CII-3 solution at 1 400 μg/mL was continuously intervened in PC3 cells for 72 hours. The cell proliferation ability was detected by CCK-8 assay and plate colony formation assay, the cell migration ability was tested by scratch wound assay, and the mRNA and protein expressions of ZAG were detected by Real time qPCR and Western blot methods, respectively. RESULTS: After PA-CII-3 intervention, the proliferation and migration abilities of PC3 cells were significantly inhibited, and the mRNA and protein expression levels of ZAG in PC3 cells were promoted. CONCLUSION: PA-CII-3 can play the role of anti-prostate cancer by inhibiting the proliferation and migration of PC3 cells, and upregulating ZAG. 

Key words: Periplaneta americana extracts, prostate cancer, zinc-alpha2-glycoprotein, cell proliferation, cell migration

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