NOD2和Toll样受体1 在哮喘大鼠中的表达及布地奈德对其的影响

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (6): 637-642.

NOD2和Toll样受体1 在哮喘大鼠中的表达及布地奈德对其的影响

张志刚¹, 叶斌¹, 童夏生², 亢晓冬³, 叶辉⁴, 王恩智⁵, 陈豪⁶, 陈琪⁷

¹台州市中心医院儿科,台州 317000,浙江;
²台州市中西医结合医院儿科,台州 317523,浙江;
³杭州师范大学钱江学院实验动物中心,杭州 310012, 浙江;
⁴台州市第一人民医院儿科,台州 317000,浙江;
⁵台州市中西医结合医院检验科,台州 317523,浙江;
⁶台州市中西医结合医院病理科,台州 317523,浙江;
⁷台州市中心医院检验科,台州 317000,浙江

收稿日期:2011-01-11 修回日期:2011-04-12 发布日期:2011-07-25
通讯作者: 童夏生,男,硕士,副主任医师,研究方向:儿童哮喘。 Tel: 0576-86440771 E-mail: xshtzg@163.com
作者简介:张志刚,男,本科,主治医师,研究方向为儿科呼吸。 Tel: 0576-88526193 E-mail: zzgtz@sina.com
基金资助:
浙江省卫生厅基金资助项目(2007-B238);温岭市科技局基金资助项目(2009-2-55)

Expressions of nucleotide-binding oligomerization domain 2 and Toll-like receptor 1 in rat asthma model and the regulation of budesonide

ZHANG Zhi-gang¹, YE Bin¹, TONG Xia-sheng², KANG Xiao-dong³, YE Hui⁴, WANG En-zhi⁵, CHEN Hao⁶, CHEN Qi⁷

¹ Department of Pediatrics,Taizhou Central Hospital, Taizhou 317000, Zhejiang, China;
² Department of Pediatrics, Taizhou Integrated Western and Traditional Chinese Medicine Hospital, Taizhou 317523,Zhejiang, China;
³ Department of Central Laboratory Animals, Qianjiang College, Hangzhou Normal University, Hangzhou 310012, Zhejiang, China;
⁴ Department of Pediatrics, Taizhou First Hospitaly, Taizhou 317000, Zhejiang,China;
⁵ Department of Clinical Laboratory, Taizhou Integrated Western and Traditional Chinese Medicine Hospital, Taizhou 317523, Zhejiang, China;
⁶ Department of Pathology, Taizhou Integrated Western and Traditional Chinese Medicine Hospital, Taizhou 317523 Zhejiang, China;
⁷ Department of Clinical Laboratory,Taizhou Central Hospital, Taizhou 317000, Zhejiang, China

Received:2011-01-11 Revised:2011-04-12 Published:2011-07-25

摘要/Abstract

摘要： 目的: 观察核苷酸结合寡聚化结构域2(NOD2)和Toll样受体1(TLR1)在哮喘大鼠中的表达及布地奈德对其的影响,探讨模式识别受体在哮喘炎症过程中的可能作用机制。方法: 采用大鼠哮喘模型,随机分成哮喘组、对照组和布地奈德组,免疫组织化学法检测肺组织NOD2的表达,流式细胞术检测血中性粒细胞(NEU)TLR1的表达。结果: 哮喘组(0.148±0.009,OD值)肺组织NOD2的光密度值显著低于对照组(0.157±0.006,OD值)(P<0.05);布地奈德组(0.149±0.008,OD值)肺组织NOD2的光密度值分别与哮喘组、对照组相比差异无统计学意义(均P>0.05)。哮喘组和布地奈德组(分别为 74.07±6.26 和 78.54±4.65,OD值)NEU TLR1的平均荧光强度均显著性低于对照组(84.37±4.96,OD值)(分别为P<0.01、P<0.05);布地奈德组NEU TLR1的平均荧光强度与哮喘组相比差异无统计学意义(P>0.05)。TLR1与NOD2的表达水平呈显著正相关(n=26,r=0.780, P<0.01)。结论: 哮喘大鼠NOD2和TLR1的表达水平下降,布地奈德可能对NOD2和TLR1的上调作用较弱,模式识别受体可能参与了哮喘的炎症过程。

关键词: 哮喘, 核苷酸结合寡聚化结构域, Toll样受体, 模式识别受体, 大鼠

Abstract: AIM: To investigate the potential roles of pattern recognition receptor in pathogenesis of asthma inflammation, the expressions of nucleotide-binding oligomerization domain 2(NOD2) and Toll-like receptor 1(TLR1) which affected by budesonide were determined in rat asthma model. METHODS: Rat models of asthma were randomly divided into three groups on average, including asthma group, control group and budesonide treated group. The expressions of NOD2 proteins were detected by immunohistochemical method, and levels of TLR1 were also measured by flow cytometry at blood neutrophil. RESULTS: The expressions of NOD2 proteins in asthma group (0.148±0.009,optical density)at lung tissure were significantly lower than those in control group(0.157±0.006, optical density)(P<0.05), but there were nonsignificance difference of NOD2 protein expressions neither between in budesonide treated group(0.149±0.008 optical density) and asthma group, nor between in budesonide treated group and control group(all P>0.05). Dramaticly, levels of TLR1 in asthma group and in budesonide treated group(74.07±6.26 and 78.54±4.65, optical density, respectively) were significantly lower than those in control group(84.37±4.96, optical density)at blood neutrophil either(P<0.01 or P<0.05, respectively).Moreover, there were a strong positive correlation between levels of NOD2 and TLR1 (n=26, r=0.780, P<0.01 ). CONCLUSION: Levels of NOD2 and TLR1 were decreased in asthmatic rats, the pattern recognition receptor may be a potential role in pathogenesis of asthma inflammation. Perhaps there is a mild effect on up-regulating NOD2 and TLR1 levels by budesonide.

Key words: Asthma, Nucleotide-binding oligomerization domain, Toll-like receptor, Pattern recognition receptor, Rat

中图分类号:

R965.2

张志刚, 叶斌, 童夏生, 亢晓冬, 叶辉, 王恩智, 陈豪, 陈琪. NOD2和Toll样受体1 在哮喘大鼠中的表达及布地奈德对其的影响[J]. 中国临床药理学与治疗学, 2011, 16(6): 637-642.

ZHANG Zhi-gang, YE Bin, TONG Xia-sheng, KANG Xiao-dong, YE Hui, WANG En-zhi, CHEN Hao, CHEN Qi. Expressions of nucleotide-binding oligomerization domain 2 and Toll-like receptor 1 in rat asthma model and the regulation of budesonide[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(6): 637-642.

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