火麻仁油对D-半乳糖致衰老小鼠学习记忆障碍的保护作用研究

中国临床药理学与治疗学 ›› 2011, Vol. 16 ›› Issue (12): 1332-1339.

火麻仁油对D-半乳糖致衰老小鼠学习记忆障碍的保护作用研究

苏婧¹, 贺海波², 石孟琼², 熊兴军³, 陈良金², 卢训丛²

¹随州市中心医院药剂科, 随州 441300,湖北;
²三峡大学医学院, 宜昌 443002,湖北;
³宜昌市长阳县中医院,长阳 443500,湖北

收稿日期:2011-08-10 修回日期:2011-11-10 出版日期:2011-12-26 发布日期:2012-01-07
通讯作者: 石孟琼,通信作者,女, 实验师, 研究方向:心脑血管药理。Tel: 13687220902 E-mail: shmq0212@126.com
作者简介:苏婧,女, 主管药师, 研究方向:中药药理。Tel: 13971791535 E-mail: 903798302@qq.com

Effects of hemp seed oil on learning and memory performances in senile mice induced by D-galactose

SU Jing¹, HE Hai-bo², SHI Meng-qiong², XIONG Xing-jun³, CHEN Liang-jin², LU Xun-cong²

¹Department of Pharmacy, Suizhou Central People's Hospital, Suizhou 441300, Hubei, China;
²Medical Science College, China Three Gorges University, Yichang 443002, Hubei, China;
³Department of Pharmacy, Traditional Chinese Medicine Hospital, Changyang 443500, Hubei, China

Received:2011-08-10 Revised:2011-11-10 Online:2011-12-26 Published:2012-01-07

摘要/Abstract

摘要： 目的: 研究火麻仁油对D-半乳糖(D-gal)致衰老小鼠学习记忆能力的保护作用,并探讨其作用机制。方法: 采用 D-半乳糖诱导小鼠衰老模型,用跳台法、水迷宫法和Morris 水迷宫法测定衰老小鼠学习记忆能力,检测小鼠脑组织丙二醛(MDA) 含量和总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及大脑皮层和海马组织中乙酰胆碱(Ach)含量和乙酰胆碱酯酶(AchE)、胆碱乙酰化转移酶(ChAT)活性,观察脑组织组织形态学变化。结果: 火麻仁油(3、6和 12 mL/kg)能明显延长跳台实验的跳台潜伏期,缩短错误次数;缩短水迷宫实验达到时间和减少错误次数;缩短Morris 水迷宫实验逃避潜伏期和第1 次到达原平台时间,增加穿越原平台次数和逗留时间;能显著增加脑组织中T-AOC、SOD、GSH-Px活性和降低MDA含量,增加大脑皮层和海马组织中Ach含量和AchE、ChAT活性,减轻脑组织损伤。结论: 火麻仁油可显著改善D-半乳糖致衰老小鼠学习记忆能力,其机制可能与其提高脑组织抗氧化和清除自由基能力,增强中枢胆碱能神经系统功能有关。

关键词: 火麻仁油, 衰老, 学习记忆, 活性氧基团, 胆碱能神经系统, 小鼠

Abstract: AIM: To research the effects of hemp seed oil on learning and memory performances in senile mice induced by D-galactose and its mechanisms.METHODS: By using the D-galactose-induced aging mice, learning and memory performances were tested with step down, water maze and Morris water maze test, and determined the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and content of malonaldehyde (MDA) in brain tissue, determined the content of acetylcholine (Ach) and activities of acetylcholine esterase (AchE), choline acetyltransferase (ChAT) in cerebral cortex and hippocampus; analyzed brain tissue histopathological examination simultaneously.RESULTS: Hemp seed oil (3, 6, 12 mL/kg) could significantly prolong the latency, reduce the error frequency in step down test, reduce the reaching time and error frequency in water maze test, obviously shorten the escape latency and reaching primary platform time, increase passing through primary platform frequency and resided time in Morris water maze test. It could obviously reduce the content of MDA, increase activities of T-AOC, SOD, GSH-Px in brain tissue, remarkably increase the content of Ach and activities of AchE, ChAT in cerebral cortex and hippocampus and reduce the brain tissue injury.CONCLUSION: Hemp seed oil improves the learning and memory performances of the D-galactose-induced aging mice. The mechanism may be related to increased activity of antioxidation and scavenge of free radicals, improved function of the central cholinesterase system.

Key words: Hemp seed oil, Senile, Learning and memory, Reactive oxygen species, Cholinesterase system, Mice

中图分类号:

R965.2

苏婧, 贺海波, 石孟琼, 熊兴军, 陈良金, 卢训丛. 火麻仁油对D-半乳糖致衰老小鼠学习记忆障碍的保护作用研究[J]. 中国临床药理学与治疗学, 2011, 16(12): 1332-1339.

SU Jing, HE Hai-bo, SHI Meng-qiong, XIONG Xing-jun, CHEN Liang-jin, LU Xun-cong. Effects of hemp seed oil on learning and memory performances in senile mice induced by D-galactose[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2011, 16(12): 1332-1339.

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