柚皮苷对人卵巢癌SKOV3细胞P38MAPK及ERK1/2蛋白表达的影响

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (5): 519-523.

柚皮苷对人卵巢癌SKOV3细胞P38MAPK及ERK1/2蛋白表达的影响

胡昕¹, 宋淑慧¹, 熊玉卿², 蔡丽萍¹

¹南昌大学第一附属医院妇产科,南昌 330006,江西;
²江西医学院临床药理研究所,南昌 330006,江西

收稿日期:2012-12-29 修回日期:2013-04-19 出版日期:2013-05-26 发布日期:2013-05-22
通讯作者: 蔡丽萍,女,本科,主任医师,教授,主要从事妇科肿瘤的临床研究。Tel: 13870931677 E-mail: Cailiping2550@163.com
作者简介:胡昕,女,硕士研究生,主要从事妇科肿瘤的临床研究。Tel: 15270873156 E-mail: huxin1516@163.com

Effects of Naringin on expression of p38MAPK and ERK1/2 signaling proteins of Human ovarian cancer cell line SKOV3

HU Xin¹, SONG Shu-hui¹, XIONG Yu-qing², CAI Li-ping¹

¹Department of Gynecology and Obstetrics, the First Affiliated Hospital of Nanchang University;
²Institute of Clinical Pharmacology, Jiangxi Medical College, Nanchang University, Nanchang 330006, Jiangxi, China

Received:2012-12-29 Revised:2013-04-19 Online:2013-05-26 Published:2013-05-22

摘要/Abstract

摘要： 目的: 探讨中药柚皮苷对人卵巢癌SKOV3细胞P38MAPK及ERK1/2蛋白表达水平的影响。方法: 体外培养人卵巢癌SKOV3细胞,分为空白对照组、柚皮苷10、20、40 μmol/L 组、塞来昔布 80 μmol/L 组。Western Blot测定培养 48 h 后各组细胞中P38MAPK及ERK1/2蛋白表达水平。结果: Western Blot结果显示,与空白对照组相比,低浓度柚皮苷组(10、20 μmol/L)对SKOV3细胞P38MAPK及ERK1/2蛋白的表达无明显影响,而 40 μmol/L 柚皮苷及塞来昔布组可明显下调P38MAPK及ERK1/2蛋白表达,具有统计学差异(P<0.05)。结论: 柚皮苷能抑制人卵巢癌SKOV3细胞P38MAPK及ERK1/2蛋白的作用,从而可能阻断卵巢癌的发生、发展。

关键词: 柚皮苷, P38MAPK, ERK1/2, 卵巢癌, SKOV3细胞

Abstract: AIM: To investigate effects of Naringin on expression of P38MAPK and ERK1/2 signaling proteins of Human ovarian cancer cell line SKOV3.METHODS: SKOV3 cell were cultured in vitro and divided into five groups randomly, namely control group, naringin group (10 μmol/L), naringin group (20 μmol/L), naringin group (40 μmol/L) and celecoxib group (80 μmol/L). The expression of P38MAPK and ERK1/2 signaling proteins were determined by Western Blot after forty-eight hours.RESULTS: Compared with control group, only naringin group (40 μmol/L) and celecoxib group can significantly cut P38MAPK and ERK1/2 signaling proteins, the difference was statistically significant (P<0.05).CONCLUSION: Naringin can inhibit P38MAPK and ERK1/2 signaling proteins of SKOV3 cell, thus may block the occur and development of ovarian cancer.

Key words: Naringin, P38MAPK, ERK1/2, Ovarian cancer, Cell SKOV3

中图分类号:

R965.2

胡昕, 宋淑慧, 熊玉卿, 蔡丽萍. 柚皮苷对人卵巢癌SKOV3细胞P38MAPK及ERK1/2蛋白表达的影响[J]. 中国临床药理学与治疗学, 2013, 18(5): 519-523.

HU Xin, SONG Shu-hui, XIONG Yu-qing, CAI Li-ping. Effects of Naringin on expression of p38MAPK and ERK1/2 signaling proteins of Human ovarian cancer cell line SKOV3[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(5): 519-523.

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