四君子汤及拆方含药血清对胃癌细胞株侧群细胞的生长抑制研究

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (5): 513-518.

四君子汤及拆方含药血清对胃癌细胞株侧群细胞的生长抑制研究

贾建光¹, 马莉², 李靖¹, 金鑫¹, 张立功¹, 刘建文², 钱军¹

¹蚌埠医学院第一附属医院肿瘤外三科,蚌埠 233004,安徽;
²华东理工大学药学院,上海 200237

收稿日期:2013-03-11 修回日期:2013-03-29 出版日期:2013-05-26 发布日期:2013-05-22
通讯作者: 钱军,男,本科,教授,研究方向:消化道恶性肿瘤的综合治疗。E-mail: qianjun512036@sina.com
作者简介:贾建光,男,博士,讲师,研究方向:消化道恶性肿瘤的综合治疗。Tel: 13955262083 E-mail: jiajianguang19782126.com
基金资助:
国家自然科学基金面上项目资助(81173386)

Experimental study of Sijunzi Decoction and its components on the growth inhibiting of the Side Population(SP) Cells in MKN-28,SGC-7901 and BGC-823

JIA Jian-guang¹, MA Li², LI Jing¹, JIN Xin¹, ZHANG Li-gong¹, LIU Jian-wen², QIAN Jun¹

¹The First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui,China;
²East China University of Science and Technology College of Pharmacy, Shanghai 200237, China

Received:2013-03-11 Revised:2013-03-29 Online:2013-05-26 Published:2013-05-22

摘要/Abstract

摘要： 目的: 考察四君子汤及拆方含药血清对胃癌细胞株侧群(Side population,SP)细胞的生长抑制作用。方法: 选择不同分化程度的人胃癌细胞株MKN-28、SGC-7901和BGC-823,以荧光染料Hoechst 33342染色,维拉帕米拮抗对照,应用流式细胞仪检测并分选出SP细胞和non-SP细胞;MTT法观察两组细胞的体外增殖活性;克隆形成实验考察两组细胞的集落形成能力;应用中药血清药理学方法,酶标仪MTT比色法观察四君子汤及拆方不同剂量、不同浓度含药血清对胃癌细胞SP细胞的生长抑制作用。结果: 胃癌细胞株MKN-28、SGC-7901和BGC-823中SP细胞比例分别为 3.40%、2.00%、1.07%,与non-SP细胞相比,SP细胞具有较强的体外增殖活性(P<0.05)和较强的克隆形成能力(P<0.01);加药组以20%高剂量血清组最为明显。四君子汤及拆方含药血清对这3株细胞的SP细胞增殖均有明显的抑制作用,呈剂量和浓度依赖性。结论: 四君子汤及拆方含药血清能明显抑制胃癌SP细胞的生长。

关键词: 四君子汤, 含药血清, 侧群细胞, MKN-28细胞, SGC-7901细胞, BGC-823细胞, 生长抑制

Abstract: AIM: To investigate the effect of Sijunzi decoction and its separate components on the growth and proliferation of the side population(SP) cells in human carcinoma cell lines MKN-28,SGC-7901 and BGC-823 in vitro.METHODS: Side population cells of three human gastric cancer cell lines with different differentiation grades named MKN-28,SGC-7901 and BGC-823,were analyzed and isolated by flow cytometryafter being stained by fluorochrome Hoechst33342 and PI, then characterized by cell growth curve (MTT) and colony formation assay. The effects of Sijunzi decoction and its separate components on the growth and proliferation of the side population(SP) cells were detected by MTT assay.RESULTS: SP cells were detected in all of the three gastric cancer cell lines with a proportion of 3.40%, 2.00%, 1.07%. Cell growth curves indicated that SP cells have stronger proliferation ability (P<0.05) compared with non-SP cells. Colony formation assay showed that the colony forming efficiency of the SP cells was higher than the total cells without sorting and the non-SP cells (P<0.01). Comparing with controls, the growth and proliferation of the SP cells were significantly inhibited by Sijunzi decoction and its separate components, and the inhibiting rates revealed in a mediated serumdose-dependent manner.CONCLUSION: Sijunzi decoction and its separate components have the growth-inhibitive effect on SP cells sorting from human carcinoma cell lines MKN-28,SGC-7901 and BGC-823 in vitro.

Key words: Sijunzi decoction, Medicated serum, Side population, MKN-28, SGC-7901, BGC-823, Growth inhibiting

中图分类号:

R965.1

贾建光, 马莉, 李靖, 金鑫, 张立功, 刘建文, 钱军. 四君子汤及拆方含药血清对胃癌细胞株侧群细胞的生长抑制研究[J]. 中国临床药理学与治疗学, 2013, 18(5): 513-518.

JIA Jian-guang, MA Li, LI Jing, JIN Xin, ZHANG Li-gong, LIU Jian-wen, QIAN Jun. Experimental study of Sijunzi Decoction and its components on the growth inhibiting of the Side Population(SP) Cells in MKN-28,SGC-7901 and BGC-823[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(5): 513-518.

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