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中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (2): 139-144.

• 基础研究 • 上一篇    下一篇

黄芪皂苷Ⅱ对人肝癌多药耐药细胞BEL-7402/FU的逆转耐药作用

王培培1, 许杜娟2, 黄灿3, 徐文科1, 栾家杰1   

  1. 1皖南医学院弋矶山医院药剂科,芜湖 241001,安徽;
    2安徽医科大学第一附属医院药剂科,国家中医药管理局,中药化学三级实验室,合肥 230022,安徽;
    3安庆市立医院药剂科,安庆 246003,安徽
  • 收稿日期:2013-06-25 修回日期:2014-02-17 出版日期:2014-02-26 发布日期:2014-03-31
  • 通讯作者: 许杜娟,女,主任药师,硕士生导师,研究方向:肿瘤药理学、临床药学。Tel: 0551-2922442 E-mail: xudujuan6365@yahoo.com.cn
  • 作者简介:王培培,女,硕士,药师,研究方向:肿瘤药理学。Tel: 0553-5739177 E-mail: wangpeipei2001@sina.com
  • 基金资助:
    安徽省自然科学基金资助项目(110406060M222);皖南医学院重点科研项目培育基金项目(WK2013ZF05)

Reversal of human hepatic cancer multidrug resistance is induced by AstragalosideⅡ in BEL-7402/FU cells

WANG Pei-pei1, XU Du-juan2, HUANG Can3, XU Wen-ke1, LUAN Jia-jie1   

  1. 1Department of Pharmacy, Yijishan Affiliated Hospital of Wannan Medical College, Wuhu 241001, Anhui, China;
    2The First Affiliated Hospital of Anhui Medical University, Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine, Hefei 230022, Anhui, China;
    3Department of Pharmacy, Anqing Shili Hospital, Anqing 246003, Anhui, China
  • Received:2013-06-25 Revised:2014-02-17 Online:2014-02-26 Published:2014-03-31

摘要: 目的: 探讨黄芪皂苷Ⅱ(AstragalosideⅡ, ASⅡ)对肝癌多药耐药性的逆转作用。方法: MTT法检测人肝癌多药耐药细胞BEL-7402/FU对化疗药物的敏感性和逆转耐药倍数,RT-PCR法检测多药耐药蛋白-1(MDR1)基因表达,免疫细胞化学法检测P-糖蛋白(P-gp)蛋白表达水平,流式细胞仪检测ASⅡ对细胞内Rhodamine123的蓄积影响。结果: BEL-7402/FU细胞对5-氟尿嘧啶(5-FU)、阿霉素、丝裂霉素的耐药倍数分别为 19.64、1.98、1.92。0.04、0.08 mg/mL 的ASⅡ能增强5-FU对BEL-7402/FU的细胞毒作用,逆转耐药倍数分别为 1.49,1.81。0.08、0.16 mg/mL 的ASⅡ作用BEL-7402/FU细胞24 h后,能下调MDR1 mRNA和P-gp蛋白表达水平,提高细胞内Rhodamine123的荧光表达率。结论: ASⅡ能部分逆转肝癌多药耐药性,其机制可能与下调MDR1 mRNA表达及抑制P-gp的功能与表达有关。

关键词: 黄芪皂苷Ⅱ, 抗药性, P-糖蛋白, 肝癌

Abstract: AIM: To investigate the effect of AstragalosideⅡ on the reversal of multidrug resistance in human hepatic cancer cells.METHODS: Susceptibility of chemotherapy drugs and relative reversal fold was evaluated by MTT. The mRNA level of MDR1 gene was detected by RT-PCR and the P-glycoprotein expression was detected by immunocytochemistry method. Intracellular Rhodamine123 accumulation was determined by flowcytometry.RESULTS: BEL-7402/FU cells showed resistance to three chemotherapy drugs 5-fluorouracil, Adriamycin and Mitomycin, their resistance index were 19.64, 1.98, 1.92. Astragaloside Ⅱ(0.04, 0.08 mg/mL) relative reversal fold were 1.49, 1.81 to 5-fluorouracil. Astragaloside Ⅱ(0.08 mg/mL, 0.16 mg/mL) could downregulate the expression of MDR1 mRNA and P-glycoprotein, increase the intracellular Rhodamine123 fluorescence.CONCLUSION: Astragaloside Ⅱ could reverse the drug resistance of Bel-7402/FU cells via regulating down the expression of MDR1 mRNA, inhibit the expression and function of P-glycoprotein.

Key words: Astragaloside Ⅱ, P-glycoprotein, multidrug resistance, hepatocellular carcinoma

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