lncFOXC1表达增加促进肝癌发生

doi:10.12092/j.issn.1009-2501.2019.01.005

中国临床药理学与治疗学 ›› 2019, Vol. 24 ›› Issue (1): 27-31.doi: 10.12092/j.issn.1009-2501.2019.01.005

lncFOXC1表达增加促进肝癌发生

周玉玲¹，刘激扬¹，姚红伟¹，徐玉芬²，张婷³，邹卓林²，潘欢²

¹中国人民武装警察部队药品仪器检验所，北京 102613； ²嘉兴市第一医院，嘉兴 314000，浙江；³北京大学医学部病原微生物学系，北京 100191

收稿日期:2018-09-03 修回日期:2018-09-29 出版日期:2019-01-26 发布日期:2019-01-25
通讯作者: 潘欢，女，硕士，研究实习员，研究方向：分子肿瘤学。 E-mail：panhuan_xue@163.com
作者简介:周玉玲，硕士，药师，研究方向：药物筛选及研发。 Tel：010-50978428 E-mail：123908111@qq.com
基金资助:
浙江省医药卫生科技计划项目（2016KYB290）；嘉兴市科技计划项目（2018AY32011）；嘉兴市第一医院院级课题（2016-YA-14）

Increased expression of lncFOXC1 promotes liver cancer formation

ZHOU Yuling¹, LIU Jiyang¹, YAO Hongwei ¹, XU Yufen², ZHANG Ting³, ZOU Zhuolin², PAN Huan²

¹ Institute of Drug Control and Instrument Maintenance of Chinese People's Armed Police Force, Beijing 102613, China; ² The First Hospital of Jiaxing, Jiaxing 314000, Zhejiang，China; ³Department of Microbiology & Infectious Disease Center, School of Basic Medical Science, Peking University Health Science Center, Beijing 100191, China

Received:2018-09-03 Revised:2018-09-29 Online:2019-01-26 Published:2019-01-25

摘要/Abstract

摘要：

目的: 探讨长链非编码RNA lncFOXC1在肝癌组织中的表达以及在肝癌干细胞生长中的作用。方法: 收集34例手术切除的肝癌组织和癌旁组织样本，实时荧光定量PCR检测lncFOXC1的表达。同时检测lncFOXC1在肝癌干细胞小球中的表达。建立lncFOXC1低表达的稳定细胞系，通过干细胞小球形成和小鼠皮下成瘤实验观察lncFOXC1对肿瘤形成的作用。结果: lncFOXC1在肝癌组织和干细胞小球中显著高表达（P<0.05）。lncFOXC1敲减后显著抑制了肝癌干细胞小球形成和小鼠皮下肿瘤的生长。结论: lncFOXC1在肝癌组织中高表达，调控肝癌干细胞生长。

关键词: lncFOXC1, 肝癌, 肿瘤干细胞

Abstract:

AIM: To investigate the expression of long noncoding RNA lncFOXC1 in liver cancer and the role in liver cancer formation. METHODS: A total of 34 liver cancer and peri-tumor tissues were collected. Expression of lncFOXC1 was detected by qRT-PCR in liver cancer and peri-tumor tissues, as well as liver cancer cell spheres. LncFOXC1 deficiency cell lines were constructed by shRNA. Then, its roles in sphere formation and in vivo tumor growth assays were studied. RESULTS: lncFOXC1 was significantly highly expressed in liver cancer tissues and liver cancer cell sphere. LncFOXC1 deficiency significantly reduced sphere formation and tumor growth. CONCLUSION: LncFOXC1 is highly expressed in liver cancer tissues, and can regulate liver cancer stem cell growth.

Key words: lncFOXC1, liver cancer, cancer stem cell

中图分类号:

R965.2
R73

周玉玲，刘激扬，姚红伟，徐玉芬，张婷，邹卓林，潘欢. lncFOXC1表达增加促进肝癌发生[J]. 中国临床药理学与治疗学, 2019, 24(1): 27-31.

ZHOU Yuling, LIU Jiyang, YAO Hongwei, XU Yufen, ZHANG Ting, ZOU Zhuolin, PAN Huan. Increased expression of lncFOXC1 promotes liver cancer formation[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(1): 27-31.

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lncFOXC1表达增加促进肝癌发生

Increased expression of lncFOXC1 promotes liver cancer formation

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