蟾毒灵对人膀胱癌T24细胞增殖和凋亡的影响

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (7): 733-737.

蟾毒灵对人膀胱癌T24细胞增殖和凋亡的影响

黄后宝, 夏东东, 沈群山, 程庆水, 王啸, 邱腾

皖南医学院第一附属医院泌尿外科,芜湖 241001,安徽

收稿日期:2013-12-31 修回日期:2014-03-28 发布日期:2014-07-21
作者简介:黄后宝,男,硕士,副教授,主任医师,硕士生导师,主要从事泌尿系统肿瘤方面研究。 Tel: 0553-5739559 E-mail: drhuanghoubao@163.com
基金资助:
安徽省高校省级自然科学研究重点项目(KJ2014A273)

Effect of bufalin on the proliferation and apoptosis of human bladder cancer T24 cells

HUANG Hou-bao, XIA Dong-dong, SHEN Qun-shan, CHEN Qing-shui, WANG Xiao, QIU Teng

Department of Urology, First Affiliated Hospital of Wannan Medical College, Wuhu 241001, Anhui,China

Received:2013-12-31 Revised:2014-03-28 Published:2014-07-21

摘要/Abstract

摘要： 目的研究蟾毒灵对人膀胱癌T24细胞增殖和凋亡的影响, 初步探讨其在膀胱癌中的抗肿瘤机制。方法采用MTT法测定蟾毒灵对人膀胱癌T24细胞增殖的影响;流式细胞术(FCM)检测蟾毒灵对癌细胞凋亡和细胞周期的影响;RT-PCR法检测癌细胞周期蛋白D1(CyclinD1)基因表达情况。结果蟾毒灵可抑制人膀胱癌T24细胞的增殖,并呈现出时间和浓度依赖性;蟾毒灵能诱导T24细胞凋亡;20 nmol/L 浓度的蟾毒灵预处理膀胱癌T24细胞后,与对照组(36.42%)进行比较,可分别在 12 h(69.76%)和 24 h(88.14%)显著诱导细胞G₀/G₁ 期阻滞(P<0.05 ),且可降低膀胱癌T24细胞的CyclinD1 mRNA 表达水平。结论蟾毒灵能显著抑制人膀胱癌T24细胞增殖,诱导T24细胞G₀/G₁ 周期阻滞,下调人膀胱癌T24细胞CyclinD1基因表达。

关键词: 膀胱癌, 蟾毒灵, 细胞周期, 细胞周期蛋白 D1

Abstract: AIM: To investigate the effect of bufalin on the proliferation of human bladder cancer T24 cells and explore the underlying anticancer mechanisms of bufalin. METHODS: The proliferation-inhibiting effect of bufalin on T24 cells was measured by methyl thiazole tetrazolium (MTT)colorimetric assay; the cell cycle distribution and apoptosis were detected by flow cytometry ( FCM ); the expression of CyclinD1 was detected by RT-PCR.RESULTS:Bufalin inhibited the proliferation of T24 cells in a time- and dose-dependent manner; bufalin could induce apoptosis of T24 cell; exposure of T24 cells to 20 nmol/L of bufalin led to significant induction of G₀/G₁ growth arrest at both 12 h (69.76%) and 24 h (88.14%),compared with negative control cells (36.42%).The mRNA levels of CyclinD1 in T24 cells was markedly decreased after treatment with bufalin.CONCLUSION: Bufalin could inhibit the proliferation and induce apoptosis of T24 cells. Bufalin could induce T24 G₀/G₁ growth arrest and decrease the mRNA levels of CyclinD1.

Key words: bladder cancer, bufalin, cell cycle, CyclinD1

中图分类号:

R965.2

黄后宝, 夏东东, 沈群山, 程庆水, 王啸, 邱腾. 蟾毒灵对人膀胱癌T24细胞增殖和凋亡的影响[J]. 中国临床药理学与治疗学, 2014, 19(7): 733-737.

HUANG Hou-bao, XIA Dong-dong, SHEN Qun-shan, CHEN Qing-shui, WANG Xiao, QIU Teng. Effect of bufalin on the proliferation and apoptosis of human bladder cancer T24 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(7): 733-737.

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