孕鼠补充牛磺酸对生长受限胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β水平的影响及临床意义探讨

doi:10.11852/zgetbjzz2017-25-01-09

中国儿童保健杂志 ›› 2017, Vol. 25 ›› Issue (1): 28-31.DOI: 10.11852/zgetbjzz2017-25-01-09

孕鼠补充牛磺酸对生长受限胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β水平的影响及临床意义探讨

王华伟^1,2,刘敬¹

1 解放军陆军总医院附属八一儿童医院新生儿重症监护中心,北京 100700;
2 苏州大学附属儿童医院新生儿科,江苏苏州 215000

收稿日期:2016-06-13 发布日期:2017-01-10 出版日期:2017-01-10
通讯作者: 刘敬,E-mail:Liujingbj@sina.com
作者简介:王华伟(1987-),男,安徽人,住院医师,硕士研究生,主要研究方向为新生儿脑损伤。
基金资助:
国家自然科学基金资助项目(81471087)

Effects and clinical significances of prenatal taurine on expressions of IL-6,IL-8,TNF-α and IL-1β in fetal rat brains of intrauterine growth restriction.

WANG Hua-wei^1,2,LIU Jing¹.

1 Bayi Children's Hospital Affiliated to the Army General Hospital of the Chinese People Liberation Army,Beijing 100700,China;
2 Children's Hospital Affiliated to Soochow University,Suzhou,Jiangsu 215000,ChinaCorresponding author:LIU Jing,E-mail:Liujingbj@sina.com

Received:2016-06-13 Online:2017-01-10 Published:2017-01-10
Contact: LIU Jing,E-mail:Liujingbj@sina.com

摘要/Abstract

摘要： 目的了解宫内生长受限(IUGR)胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β表达水平,探讨孕鼠补充牛磺酸对其表达水平的影响及临床意义。方法采用妊娠期全程饥饿法建立IUGR胎鼠模型,将15只孕鼠随机分为3组正常对照组、IUGR组、IUGR＋补充牛磺酸组[300 mg/(kg·d)](牛磺酸组)。采用酶联免疫吸附法测定三组胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β的含量。结果对照组、IUGR组、牛磺酸组胎鼠脑组织IL-6含量分别为(81.070±5.286)pmol/L、(148.362±18.733)pmol/L和(107.361±11.184)pmol/L,差异有统计学意义(H=43.760,P=0.00);三组胎鼠脑组织IL-8含量分别为(90.573±7.344)pmol/L、(165.596±8.635)pmol/L和(126.309±11.008)pmol/L,差异有统计学意义(H=45.910,P=0.00);三组胎鼠脑组织TNF-α含量分别为(8.231±0.607)pmol/L、(14.881±0.703)pmol/L和(11.709±0.936)pmol/L,差异有统计学意义(H=45.929,P=0.00);三组胎鼠脑组织IL-1β含量分别为(46.693±4.122)pmol/L、(84.799±4.315)pmol/L和(62.407±4.631)pmol/L,差异有统计学意义(H=46.064,P=0.00)。结论孕鼠补充牛磺酸可减少IUGR胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β的表达。

关键词: 宫内生长受限, 胎鼠, 牛磺酸, 白介素-6, 白介素-8, 肿瘤坏死因子-α, 白介素-1β

Abstract: Objectives To explore the effects and clinical significance of prenatal taurine on IL-6,IL-8,TNF-α and IL-1β in fetal rat brains of intrauterine growth restriction(IUGR). Methods IUGR was induced by food restriction throughout pregnancy.Pregnant rats were randomly divided into 3 groups:control group,IUGR group and taurine group[300 mg/(kg·d)].The expressions of serum IL-6,IL-8,TNF-α and IL-1β in brains were detected by Enzyme linked immunosorbent- assay(ELISA). Results The expressions of IL-6 in control group,test group and taurine group were:(81.070±5.286)pmol/L,(148.362±18.733)pmol/L and (107.361±11.184)pmol/L,there were significant differences among the three groups (H=43.760,P=0.00).The contents of IL-8 in three groups were:(90.573±7.344)pmol/L,(165.596±8.635)pmol/L and (126.309±11.008)pmol/L.There were significant differences among the three groups (H=45.910,P=0.00).The expressions of TNF-α in three groups were:(8.231±0.607)pmol/L,(14.881±0.703)pmol/L and (11.709±0.936)pmol/L,there were significant differences among the three groups (H=45.929,P=0.00).The expressions of IL-1β in three groups were:(46.693±4.122)pmol/L,(84.799±4.315)pmol/L and (62.407±4.631)pmol/L,there were significant differences among the three groups (H=46.064,P=0.00). Conclusion Prenatal taurine can reduce expressions IL-6,IL-8,TNF-α and IL-1β in fetal rat brains of IUGR.

Key words: intrauterine growth restriction, fetal rat, taurine, interleukin-6, interleukin-8, tumor necrosis factor alpha, interleukin-1β

中图分类号:

R179

王华伟,刘敬. 孕鼠补充牛磺酸对生长受限胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β水平的影响及临床意义探讨[J]. 中国儿童保健杂志, 2017, 25(1): 28-31.

WANG Hua-wei,LIU Jing.. Effects and clinical significances of prenatal taurine on expressions of IL-6,IL-8,TNF-α and IL-1β in fetal rat brains of intrauterine growth restriction.[J]. journal1, 2017, 25(1): 28-31.

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孕鼠补充牛磺酸对生长受限胎鼠脑组织IL-6、IL-8、TNF-α和IL-1β水平的影响及临床意义探讨

Effects and clinical significances of prenatal taurine on expressions of IL-6,IL-8,TNF-α and IL-1β in fetal rat brains of intrauterine growth restriction.

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